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Article

Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells

  • Authors:
    • Zhe Chen
    • Hongfei Jin
    • Yahui Hou
    • Bo Wang
    • Chunlin Chen
    • Ziyi Yang
    • Dongbing Ding
    • Yunfei Zuo
    • Shuangyi Ren
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China, Department of Clinical Biochemistry, Dalian Medical University, Dalian, Liaoning 116044, P.R. China
  • Pages: 1542-1546
    |
    Published online on: October 27, 2014
       https://doi.org/10.3892/mmr.2014.2776
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Abstract

The aims of the current study were to systematically analyze the regulation of the expression of NALP3 by the P2X7 receptor in P388D1 murine macrophage‑like cells, and to investigate the association between the P2X7 receptor and the NALP3 at the molecular level. Cell culture, RNA transfection, adenosine triphosphate (ATP)‑induced expression of NALP3, reverse transcription polymerase chain reaction and western blotting were used to explore the association between the P2X7 receptor and NALP3‑encoding gene in P388D1 murine macrophage‑like cells at the molecular level. The ATP‑activated P2X7 receptor can induce the upregulation of NALP3 expression at the gene and protein levels in P388D1 cells. These results demonstrated that the activation of P2X7 increases the expression levels of NALP3 in P388D1 murine macrophage‑like cells.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Z, Jin H, Hou Y, Wang B, Chen C, Yang Z, Ding D, Zuo Y and Ren S: Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells. Mol Med Rep 11: 1542-1546, 2015.
APA
Chen, Z., Jin, H., Hou, Y., Wang, B., Chen, C., Yang, Z. ... Ren, S. (2015). Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells. Molecular Medicine Reports, 11, 1542-1546. https://doi.org/10.3892/mmr.2014.2776
MLA
Chen, Z., Jin, H., Hou, Y., Wang, B., Chen, C., Yang, Z., Ding, D., Zuo, Y., Ren, S."Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells". Molecular Medicine Reports 11.2 (2015): 1542-1546.
Chicago
Chen, Z., Jin, H., Hou, Y., Wang, B., Chen, C., Yang, Z., Ding, D., Zuo, Y., Ren, S."Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells". Molecular Medicine Reports 11, no. 2 (2015): 1542-1546. https://doi.org/10.3892/mmr.2014.2776
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Z, Jin H, Hou Y, Wang B, Chen C, Yang Z, Ding D, Zuo Y and Ren S: Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells. Mol Med Rep 11: 1542-1546, 2015.
APA
Chen, Z., Jin, H., Hou, Y., Wang, B., Chen, C., Yang, Z. ... Ren, S. (2015). Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells. Molecular Medicine Reports, 11, 1542-1546. https://doi.org/10.3892/mmr.2014.2776
MLA
Chen, Z., Jin, H., Hou, Y., Wang, B., Chen, C., Yang, Z., Ding, D., Zuo, Y., Ren, S."Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells". Molecular Medicine Reports 11.2 (2015): 1542-1546.
Chicago
Chen, Z., Jin, H., Hou, Y., Wang, B., Chen, C., Yang, Z., Ding, D., Zuo, Y., Ren, S."Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells". Molecular Medicine Reports 11, no. 2 (2015): 1542-1546. https://doi.org/10.3892/mmr.2014.2776
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