Purification of polysaccharides from Cordyceps militaris and their anti‑hypoxic effect

  • Authors:
    • Yuan Dong
    • Shuang Hu
    • Chungang Liu
    • Qingfan Meng
    • Jia Song
    • Jiahui  Lu
    • Yingkun Cheng
    • Chaohui Gao
    • Yan Liu
    • Di Wang
    • Lirong Teng
  • View Affiliations

  • Published online on: October 27, 2014     https://doi.org/10.3892/mmr.2014.2786
  • Pages: 1312-1317
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Abstract

Acute mountain sickness, one of the most common altitude diseases, causes lung and brain injury. The present study aimed to investigate the anti‑hypoxic effect of purified polysaccharides extracted from Cordyceps militaris. The aqueous extract of Cordyceps militaris was purified progressively through a DEAE‑52 cellulose anion exchange column and a Sepharose G‑100 column. The fraction CMN1, with a molecular weight of 37842 Da, was the main fraction obtained and its chemical composition and structural characteristics were determined. CMN1 was found to have a monosaccharide composition of L‑rhamnose, L‑arabinose, D‑mannose, D‑galactose. The backbone of CMN1 comprised (1→2) and (1→3) linkages, with branched (1→6) and (1→4) linkages. The anti‑hypoxic effects of CMN1 were determined using a sodium nitrite toxicosis test, acute cerebral ischemic/hypoxic test and normobarie hypoxia test. CMN1 (0.5 g/kg) possessed a similar anti‑hypoxic effect to rhodiola oral liquid. Overall, the Cordyceps militaris polysaccharide, CMN1, was identified as an effective agent against hypoxia.
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February-2015
Volume 11 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Dong Y, Hu S, Liu C, Meng Q, Song J, Lu J, Cheng Y, Gao C, Liu Y, Wang D, Wang D, et al: Purification of polysaccharides from Cordyceps militaris and their anti‑hypoxic effect. Mol Med Rep 11: 1312-1317, 2015
APA
Dong, Y., Hu, S., Liu, C., Meng, Q., Song, J., Lu, J. ... Teng, L. (2015). Purification of polysaccharides from Cordyceps militaris and their anti‑hypoxic effect. Molecular Medicine Reports, 11, 1312-1317. https://doi.org/10.3892/mmr.2014.2786
MLA
Dong, Y., Hu, S., Liu, C., Meng, Q., Song, J., Lu, J., Cheng, Y., Gao, C., Liu, Y., Wang, D., Teng, L."Purification of polysaccharides from Cordyceps militaris and their anti‑hypoxic effect". Molecular Medicine Reports 11.2 (2015): 1312-1317.
Chicago
Dong, Y., Hu, S., Liu, C., Meng, Q., Song, J., Lu, J., Cheng, Y., Gao, C., Liu, Y., Wang, D., Teng, L."Purification of polysaccharides from Cordyceps militaris and their anti‑hypoxic effect". Molecular Medicine Reports 11, no. 2 (2015): 1312-1317. https://doi.org/10.3892/mmr.2014.2786