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Article

LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro

  • Authors:
    • Yang Zhou
    • Liang Bo Zhu
    • Ai Fen Peng
    • Tao Fang Wang
    • Xin Hua Long
    • Song Gao
    • Rong Ping Zhou
    • Zhi Li Liu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, College of Humanities, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330006, P.R. China, Department of Orthopedics, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
  • Pages: 1352-1357
    |
    Published online on: October 27, 2014
       https://doi.org/10.3892/mmr.2014.2787
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Abstract

Increasing evidence suggests that fatty acid synthase (FASN) is crucial in the carcinogenesis of various types of tumor. In addition, the phosphatidylinositol 3‑kinase (PI3K)/Akt signaling pathway, which is closely associated with cellular metabolism, affects cancer biology. However, whether the malignant phenotype of osteosarcoma (OS) cells is regulated by the PI3K/Akt/FASN signaling pathway and how the PI3K family specific inhibitor, 2‑(4‑morpholinyl)‑8‑phenyl‑chromone (LY294002) affects the malignant phenotype of OS cells remains to be elucidated. In the present study, U2‑OS and MG‑63 cells were treated with LY294002 and subsequently western blot analysis was used to examine Akt, p‑Akt and FASN protein expression. Additionally, FASN mRNA was detected by reverse transcription quantitative polymerase chain reaction. MTT and fluorescence‑activated cell sorting assays were used to assess proliferation and apoptosis. Migration and invasion were investigated using wound healing and transwell invasion assays. The results demonstrated that LY294002 suppressed the PI3K/Akt/FASN signaling pathway. However, the malignant phenotypes of OS cells mentioned above were significantly inhibited. The present results indicated that LY294002 inhibits the malignant phenotype of OS cells via modulation of the PI3K/Akt/FASN signaling pathway in vitro and may be a new therapeutic strategy for the management of OS.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Y, Zhu LB, Peng AF, Wang TF, Long XH, Gao S, Zhou RP and Liu ZL: LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro. Mol Med Rep 11: 1352-1357, 2015.
APA
Zhou, Y., Zhu, L.B., Peng, A.F., Wang, T.F., Long, X.H., Gao, S. ... Liu, Z.L. (2015). LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro. Molecular Medicine Reports, 11, 1352-1357. https://doi.org/10.3892/mmr.2014.2787
MLA
Zhou, Y., Zhu, L. B., Peng, A. F., Wang, T. F., Long, X. H., Gao, S., Zhou, R. P., Liu, Z. L."LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro". Molecular Medicine Reports 11.2 (2015): 1352-1357.
Chicago
Zhou, Y., Zhu, L. B., Peng, A. F., Wang, T. F., Long, X. H., Gao, S., Zhou, R. P., Liu, Z. L."LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro". Molecular Medicine Reports 11, no. 2 (2015): 1352-1357. https://doi.org/10.3892/mmr.2014.2787
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Y, Zhu LB, Peng AF, Wang TF, Long XH, Gao S, Zhou RP and Liu ZL: LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro. Mol Med Rep 11: 1352-1357, 2015.
APA
Zhou, Y., Zhu, L.B., Peng, A.F., Wang, T.F., Long, X.H., Gao, S. ... Liu, Z.L. (2015). LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro. Molecular Medicine Reports, 11, 1352-1357. https://doi.org/10.3892/mmr.2014.2787
MLA
Zhou, Y., Zhu, L. B., Peng, A. F., Wang, T. F., Long, X. H., Gao, S., Zhou, R. P., Liu, Z. L."LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro". Molecular Medicine Reports 11.2 (2015): 1352-1357.
Chicago
Zhou, Y., Zhu, L. B., Peng, A. F., Wang, T. F., Long, X. H., Gao, S., Zhou, R. P., Liu, Z. L."LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3‑kinase/Akt/fatty acid synthase signaling pathway in vitro". Molecular Medicine Reports 11, no. 2 (2015): 1352-1357. https://doi.org/10.3892/mmr.2014.2787
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