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Article

GRIM‑19‑mediated Stat3 activation is a determinant for resveratrol‑induced proliferation and cytotoxicity in cervical tumor‑derived cell lines

  • Authors:
    • Yong‑Guang Li
    • Hong‑Juan Xia
    • Jian‑Ping Tao
    • Ping Xin
    • Ming‑Ya Liu
    • Jing‑Bo Li
    • Wei Zhu
    • Meng Wei
  • View Affiliations / Copyright

    Affiliations: Division of Cardiology, Sixth Hospital, Shanghai Jiao Tong University, State Key Discipline Division, Shanghai 200233, P.R. China
  • Pages: 1272-1277
    |
    Published online on: October 29, 2014
       https://doi.org/10.3892/mmr.2014.2797
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Abstract

Resveratrol is a natural phenol, produced from red grapes, berries and peanuts. Previous studies have suggested that resveratrol exerts anticancer effects. Activation of the signal transducer and activator of transcription 3 (Stat3) is important in cancer. However, the mechanisms by which resveratrol suppresses the Stat3 signaling pathway remain to be elucidated. The aim of the present study was to investigate the effects of resveratrol on GRIM‑19‑Stat3 signaling in HeLa cells, derived from a cervical tumor. HeLa cells were divided into experimental groups and treated with resveratrol. Western blotting was used to analyze the expression levels of p‑Stat3, Stat3, GRIM‑19 and β‑actin. Cell viability was determined using an MTT assay. The results showed that 100 µM resveratrol suppressed the proliferation and Stat3 phosphorylation in HeLa cells, and induced the expression of the gene associated with retinoid‑IFN‑induced mortality 19 (GRIM‑19) protein. Overexpression of GRIM‑19 suppressed the Stat3 signaling pathway in HeLa cells. The Stat3 signaling pathway was activated following the downregulation of GRIM‑19 expression using short interfering RNAs (siRNAs). Resveratrol suppressed cell proliferation, however, this effect was decreased through the use of siRNAs. The suppression of Stat3 phosphorylation by resveratrol decreased following treatment with siRNAs. To the best of our knowledge, the present study is among the first to identify GRIM‑19‑Stat3 signaling as a target of resveratrol, and further elucidates the mechanisms underlying the antitumor activity of resveratrol.
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Copy and paste a formatted citation
Spandidos Publications style
Li YG, Xia HJ, Tao JP, Xin P, Liu MY, Li JB, Zhu W and Wei M: GRIM‑19‑mediated Stat3 activation is a determinant for resveratrol‑induced proliferation and cytotoxicity in cervical tumor‑derived cell lines. Mol Med Rep 11: 1272-1277, 2015.
APA
Li, Y., Xia, H., Tao, J., Xin, P., Liu, M., Li, J. ... Wei, M. (2015). GRIM‑19‑mediated Stat3 activation is a determinant for resveratrol‑induced proliferation and cytotoxicity in cervical tumor‑derived cell lines. Molecular Medicine Reports, 11, 1272-1277. https://doi.org/10.3892/mmr.2014.2797
MLA
Li, Y., Xia, H., Tao, J., Xin, P., Liu, M., Li, J., Zhu, W., Wei, M."GRIM‑19‑mediated Stat3 activation is a determinant for resveratrol‑induced proliferation and cytotoxicity in cervical tumor‑derived cell lines". Molecular Medicine Reports 11.2 (2015): 1272-1277.
Chicago
Li, Y., Xia, H., Tao, J., Xin, P., Liu, M., Li, J., Zhu, W., Wei, M."GRIM‑19‑mediated Stat3 activation is a determinant for resveratrol‑induced proliferation and cytotoxicity in cervical tumor‑derived cell lines". Molecular Medicine Reports 11, no. 2 (2015): 1272-1277. https://doi.org/10.3892/mmr.2014.2797
Copy and paste a formatted citation
x
Spandidos Publications style
Li YG, Xia HJ, Tao JP, Xin P, Liu MY, Li JB, Zhu W and Wei M: GRIM‑19‑mediated Stat3 activation is a determinant for resveratrol‑induced proliferation and cytotoxicity in cervical tumor‑derived cell lines. Mol Med Rep 11: 1272-1277, 2015.
APA
Li, Y., Xia, H., Tao, J., Xin, P., Liu, M., Li, J. ... Wei, M. (2015). GRIM‑19‑mediated Stat3 activation is a determinant for resveratrol‑induced proliferation and cytotoxicity in cervical tumor‑derived cell lines. Molecular Medicine Reports, 11, 1272-1277. https://doi.org/10.3892/mmr.2014.2797
MLA
Li, Y., Xia, H., Tao, J., Xin, P., Liu, M., Li, J., Zhu, W., Wei, M."GRIM‑19‑mediated Stat3 activation is a determinant for resveratrol‑induced proliferation and cytotoxicity in cervical tumor‑derived cell lines". Molecular Medicine Reports 11.2 (2015): 1272-1277.
Chicago
Li, Y., Xia, H., Tao, J., Xin, P., Liu, M., Li, J., Zhu, W., Wei, M."GRIM‑19‑mediated Stat3 activation is a determinant for resveratrol‑induced proliferation and cytotoxicity in cervical tumor‑derived cell lines". Molecular Medicine Reports 11, no. 2 (2015): 1272-1277. https://doi.org/10.3892/mmr.2014.2797
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