Chondrogenic differentiation of human adipose‑derived stem cells using microcarrier and bioreactor combination technique

Kang,Hongjun; Lu,Shibi; Peng,Jiang; Yang,Qiang; Liu,Shuyun; Zhang,Li; Huang,Jingxiang; Sui,Xiang; Zhao,Bin; Wang,Aiyuan; Xu,Wenjing; Guo,Quanyi; Song,Qing

doi:10.3892/mmr.2014.2820

February-2015 Volume 11 Issue 2

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February-2015 Volume 11 Issue 2

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Article

Chondrogenic differentiation of human adipose‑derived stem cells using microcarrier and bioreactor combination technique

Authors:
- Hongjun Kang
- Shibi Lu
- Jiang Peng
- Qiang Yang
- Shuyun Liu
- Li Zhang
- Jingxiang Huang
- Xiang Sui
- Bin Zhao
- Aiyuan Wang
- Wenjing Xu
- Quanyi Guo
- Qing Song
View Affiliations / Copyright

Affiliations: Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, P.R. China, Department of Spine Surgery, Tianjin Hospital, Hexi, Tianjin 300211, P.R. China, Critical Care Medicine, Chinese PLA General Hospital, Beijing 100853, P.R. China
Pages: 1195-1199
|
Published online on: October 30, 2014

https://doi.org/10.3892/mmr.2014.2820
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Abstract

The aim of the current study was to explore the application of microcarrier technology in the rapid amplification and chondrogenic differentiation of human adipose‑derived stem cells (ADSCs) in a rotating bioreactor. Human ADSCs were cultivated with Cytodex 3 microcarriers in a rotary cell culture system (RCCS), and using inverted and scanning electron microscopes, the ADSCs were observed on the surface of the microcarriers. The harvested ADSCs were stained with safranin‑O or toluidine blue histochemical stains, and type II collagen immunohistochemical stain. ADSCs were adherent to the surface of Cytodex 3 microcarriers by 24 h. They became short and spindle‑shaped, and as time progressed, the adherence of the cells to the microcarriers gradually improved. By the end of the culture period, the cell densities were ~19 times that of the initial cell density. The harvested cells on microcarriers were safranin-O and toluidine blue staining and collagen Ⅱ‑positive staining, which were stronger than the control group. The application of microcarrier technology is able to rapidly amplify human ADSC proliferation and successfully implement chondrogenic differentiation in vitro.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Chondrogenic differentiation of human adipose‑derived stem cells using microcarrier and bioreactor combination technique

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