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Article

microRNA-34a and microRNA-34c promote the activation of human hepatic stellate cells by targeting peroxisome proliferator-activated receptor γ

  • Authors:
    • Xiaofei Li
    • Yongxin Chen
    • Shuang Wu
    • Jinke He
    • Lianqing Lou
    • Weiwei Ye
    • Jinhe Wang
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Diseases, Yiwu Central Hospital, Yiwu, Zhejiang 322000, P.R. China
  • Pages: 1017-1024
    |
    Published online on: November 3, 2014
       https://doi.org/10.3892/mmr.2014.2846
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Abstract

Liver fibrosis is the common outcome of almost all cases of chronic liver disease. The hallmark of liver fibrosis is the activation of hepatic stellate cells (HSCs). microRNA‑34a (miR‑34a), which regulates a plethora of target proteins involved in the cell cycle, apoptosis, differentiation and cellular development, is found to be upregulated in both activated HSCs and liver fibrosis, while it is consistently downregulated in numerous cancer types. In the present study, the possible mechanisms underlying the role of miR‑34a and miR‑34c in the activation of the HSCs was investigated. Through bioinformatics analysis and a luciferase reporter assay, five genes were identified to be the target genes of miR‑34a and miR‑34c. Of these, peroxisome proliferator‑activated receptor γ (PPARγ) was selected for further investigation. Mutation luciferase reporter assay confirmed the direct interaction of PPARγ and miR‑34a and miR‑34c. Western blot analysis and quantitative polymerase chain reaction demonstrated that the expression of PPARγ was negatively correlated with the expression of miR‑34a and miR‑34c during the activation of HSCs. In activated human HSCs, inhibitors of miR‑34a and miR‑34c upregulated the expression of PPARγ and downregulated the expression of α‑smooth muscle actin. These data suggested that the miR‑34 family may be involved the process of liver fibrosis by targeting PPARγ.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Chen Y, Wu S, He J, Lou L, Ye W and Wang J: microRNA-34a and microRNA-34c promote the activation of human hepatic stellate cells by targeting peroxisome proliferator-activated receptor γ. Mol Med Rep 11: 1017-1024, 2015.
APA
Li, X., Chen, Y., Wu, S., He, J., Lou, L., Ye, W., & Wang, J. (2015). microRNA-34a and microRNA-34c promote the activation of human hepatic stellate cells by targeting peroxisome proliferator-activated receptor γ. Molecular Medicine Reports, 11, 1017-1024. https://doi.org/10.3892/mmr.2014.2846
MLA
Li, X., Chen, Y., Wu, S., He, J., Lou, L., Ye, W., Wang, J."microRNA-34a and microRNA-34c promote the activation of human hepatic stellate cells by targeting peroxisome proliferator-activated receptor γ". Molecular Medicine Reports 11.2 (2015): 1017-1024.
Chicago
Li, X., Chen, Y., Wu, S., He, J., Lou, L., Ye, W., Wang, J."microRNA-34a and microRNA-34c promote the activation of human hepatic stellate cells by targeting peroxisome proliferator-activated receptor γ". Molecular Medicine Reports 11, no. 2 (2015): 1017-1024. https://doi.org/10.3892/mmr.2014.2846
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Chen Y, Wu S, He J, Lou L, Ye W and Wang J: microRNA-34a and microRNA-34c promote the activation of human hepatic stellate cells by targeting peroxisome proliferator-activated receptor γ. Mol Med Rep 11: 1017-1024, 2015.
APA
Li, X., Chen, Y., Wu, S., He, J., Lou, L., Ye, W., & Wang, J. (2015). microRNA-34a and microRNA-34c promote the activation of human hepatic stellate cells by targeting peroxisome proliferator-activated receptor γ. Molecular Medicine Reports, 11, 1017-1024. https://doi.org/10.3892/mmr.2014.2846
MLA
Li, X., Chen, Y., Wu, S., He, J., Lou, L., Ye, W., Wang, J."microRNA-34a and microRNA-34c promote the activation of human hepatic stellate cells by targeting peroxisome proliferator-activated receptor γ". Molecular Medicine Reports 11.2 (2015): 1017-1024.
Chicago
Li, X., Chen, Y., Wu, S., He, J., Lou, L., Ye, W., Wang, J."microRNA-34a and microRNA-34c promote the activation of human hepatic stellate cells by targeting peroxisome proliferator-activated receptor γ". Molecular Medicine Reports 11, no. 2 (2015): 1017-1024. https://doi.org/10.3892/mmr.2014.2846
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