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Article

Hiwi downregulation, mediated by shRNA, reduces the proliferation and migration of human hepatocellular carcinoma cells

  • Authors:
    • Yingjun Xie
    • Yongsheng Yang
    • Degang Ji
    • Dan Zhang
    • Xiaoxiao Yao
    • Xuewen Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary‑Pancreatic Surgery, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
  • Pages: 1455-1461
    |
    Published online on: November 3, 2014
       https://doi.org/10.3892/mmr.2014.2847
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Abstract

The Piwi subfamily is one of two Argonaute family proteins, which are characterized by the presence of Piwi and Piwi‑Argonaute‑Zwille domains, and are well known for their role in RNA silencing. Hiwi, a human member of the Piwi subfamily, is restricted to the germ line, where it binds Piwi‑interacting RNAs and functions in stem cell self‑renewal and gametogenesis. Previous reports have indicated that abnormal Hiwi expression may be associated with a poor prognosis of numerous types of human cancer, including hepatocellular carcinoma (HCC). However, little is currently known about the oncogenic role of Hiwi in HCC. In the present study, it was confirmed that Hiwi is overexpressed at both the mRNA and protein level, in HCC specimens, as well as in MHCC97L and MHCC97H HCC cell lines. A lentivirus‑mediated small hairpin rna (shRNA) targeting Hiwi was constructed and used to infect MHCC97L and MHCC97H cells. Relative Hiwi mRNA and protein expression levels were determined by quantitative polymerase chain reaction and western blot analysis, respectively. Cell proliferation, migration and invasion were determined using cell count, scratch and Transwell assays, respectively. Hiwi mRNA and protein expression was significantly downregulated in HCC cells in response to transduction with the lentivirus‑mediated shRNA. Furthermore, the proliferative, migrative and invasive properties of the shRNA‑transduced cells were significantly decreased. Therefore, Hiwi downregulation mediated by shRNA, may reduce the proliferation and migration of HCC cells. These results indicate that Hiwi may have an important role in the progression of HCC and may be a target for anticancer therapy.
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1 

Bohmert K, Camus I, Bellini C, Bouchez D, Caboche M and Benning C: AGO1 defines a novel locus of Arabidopsis controlling leaf development. EMBO J. 17:170–180. 1998. View Article : Google Scholar : PubMed/NCBI

2 

Hall TM: Structure and function of argonaute proteins. Structure. 13:1403–1408. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Carmell MA, Xuan Z, Zhang MQ and Hannon GJ: The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis. Genes Dev. 16:2733–2742. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Meister G and Tuschl T: Mechanisms of gene silencing by double-stranded RNA. Nature. 431:343–349. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Hutvagner G and Simard MJ: Argonaute proteins: key players in RNA silencing. Nat Rev Mol Cell Biol. 9:22–32. 2008. View Article : Google Scholar

6 

Peters L and Meister G: Argonaute proteins: mediators of RNA silencing. Mol Cell. 26:611–623. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Höck J and Meister G: The Argonaute protein family. Genome Biol. 9:2102008. View Article : Google Scholar : PubMed/NCBI

8 

Houwing S, Kamminga LM, Berezikov E, et al: A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish. Cell. 129:69–82. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K and Tuschl T: Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:494–498. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Sana J, Faltejskova P, Svoboda M and Slaby O: Novel classes of non-coding RNAs and cancer. J Transl Med. 10:1032012. View Article : Google Scholar : PubMed/NCBI

11 

Qiao D, Zeeman AM, Deng W, Looijenga LH and Lin H: Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas. Oncogene. 21:3988–3999. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Skotheim RI, Kraggerud SM, Fossa SD, et al: Familial/bilateral and sporadic testicular germ cell tumors show frequent genetic changes at loci with suggestive linkage evidence. Neoplasia. 3:196–203. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Summersgill B, Osin P, Lu YJ, Huddart R and Shipley J: Chromosomal imbalances associated with carcinoma in situ and associated testicular germ cell tumours of adolescents and adults. Br J Cancer. 85:213–220. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Grochola LF, Greither T, Taubert H, et al: The stem cell-associated Hiwi gene in human adenocarcinoma of the pancreas: expression and risk of tumour-related death. Br J Cancer. 99:1083–1088. 2008. View Article : Google Scholar : PubMed/NCBI

15 

He W, Wang Z, Wang Q, et al: Expression of HIWI in human esophageal squamous cell carcinoma is significantly associated with poorer prognosis. BMC Cancer. 9:4262009. View Article : Google Scholar : PubMed/NCBI

16 

Oh SJ, Kim SM, Kim YO and Chang HK: Clinicopathologic implications of PIWIL2 expression in colorectal cancer. Korean J Pathol. 46:318–323. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Liu X, Sun Y, Guo J, et al: Expression of hiwi gene in human gastric cancer was associated with proliferation of cancer cells. Int J Cancer. 118:1922–1929. 2006. View Article : Google Scholar

18 

Jiang J, Zhang H, Tang Q, Hao B and Shi R: Expression of HIWI in human hepatocellular carcinoma. Cell Biochem Biophys. 61:53–58. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Zhao YM, Zhou JM, Wang LR, et al: HIWI is associated with prognosis in patients with hepatocellular carcinoma after curative resection. Cancer. 118:2708–2717. 2012. View Article : Google Scholar

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

21 

Li Z, Tian T, Lv F, et al: Six1 promotes proliferation of pancreatic cancer cells via upregulation of cyclin D1 expression. PLoS One. 8:e592032013. View Article : Google Scholar : PubMed/NCBI

22 

Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Xu C, Liu S, Fu H, et al: MicroRNA-193b regulates proliferation, migration and invasion in human hepatocellular carcinoma cells. Eur J Cancer. 46:2828–2836. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Aravalli RN, Steer CJ and Cressman EN: Molecular mechanisms of hepatocellular carcinoma. Hepatology. 48:2047–2063. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Schafer DF and Sorrell MF: Hepatocellular carcinoma. Lancet. 353:1253–1257. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Thorgeirsson SS and Grisham JW: Molecular pathogenesis of human hepatocellular carcinoma. Nat Genet. 31:339–346. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Llovet JM, Burroughs A and Bruix J: Hepatocellular carcinoma. Lancet. 362:1907–1917. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Iizuka N, Oka M, Yamada-Okabe H, et al: Comparison of gene expression profiles between hepatitis B virus- and hepatitis C virus-infected hepatocellular carcinoma by oligonucleotide microarray data on the basis of a supervised learning method. Cancer Res. 62:3939–3944. 2002.PubMed/NCBI

29 

Oliva J, Bardag-Gorce F, French BA, et al: Fat10 is an epigenetic marker for liver preneoplasia in a drug-primed mouse model of tumorigenesis. Exp Mol Pathol. 84:102–112. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Huang J, Zheng DL, Qin FS, et al: Genetic and epigenetic silencing of SCARA5 may contribute to human hepatocellular carcinoma by activating FAK signaling. J Clin Invest. 120:223–241. 2010. View Article : Google Scholar :

31 

Huang J, Zhang X, Zhang M, et al: Up-regulation of DLK1 as an imprinted gene could contribute to human hepatocellular carcinoma. Carcinogenesis. 28:1094–1103. 2007. View Article : Google Scholar

32 

Okada T, Iizuka N, Yamada-Okabe H, et al: Gene expression profile linked to p53 status in hepatitis C virus-related hepatocellular carcinoma. FEBS Lett. 555:583–590. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Schnabl B, Valletta D, Kirovski G and Hellerbrand C: Zinc finger protein 267 is up-regulated in hepatocellular carcinoma and promotes tumor cell proliferation and migration. Exp Mol Pathol. 91:695–701. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xie Y, Yang Y, Ji D, Zhang D, Yao X and Zhang X: Hiwi downregulation, mediated by shRNA, reduces the proliferation and migration of human hepatocellular carcinoma cells. Mol Med Rep 11: 1455-1461, 2015.
APA
Xie, Y., Yang, Y., Ji, D., Zhang, D., Yao, X., & Zhang, X. (2015). Hiwi downregulation, mediated by shRNA, reduces the proliferation and migration of human hepatocellular carcinoma cells. Molecular Medicine Reports, 11, 1455-1461. https://doi.org/10.3892/mmr.2014.2847
MLA
Xie, Y., Yang, Y., Ji, D., Zhang, D., Yao, X., Zhang, X."Hiwi downregulation, mediated by shRNA, reduces the proliferation and migration of human hepatocellular carcinoma cells". Molecular Medicine Reports 11.2 (2015): 1455-1461.
Chicago
Xie, Y., Yang, Y., Ji, D., Zhang, D., Yao, X., Zhang, X."Hiwi downregulation, mediated by shRNA, reduces the proliferation and migration of human hepatocellular carcinoma cells". Molecular Medicine Reports 11, no. 2 (2015): 1455-1461. https://doi.org/10.3892/mmr.2014.2847
Copy and paste a formatted citation
x
Spandidos Publications style
Xie Y, Yang Y, Ji D, Zhang D, Yao X and Zhang X: Hiwi downregulation, mediated by shRNA, reduces the proliferation and migration of human hepatocellular carcinoma cells. Mol Med Rep 11: 1455-1461, 2015.
APA
Xie, Y., Yang, Y., Ji, D., Zhang, D., Yao, X., & Zhang, X. (2015). Hiwi downregulation, mediated by shRNA, reduces the proliferation and migration of human hepatocellular carcinoma cells. Molecular Medicine Reports, 11, 1455-1461. https://doi.org/10.3892/mmr.2014.2847
MLA
Xie, Y., Yang, Y., Ji, D., Zhang, D., Yao, X., Zhang, X."Hiwi downregulation, mediated by shRNA, reduces the proliferation and migration of human hepatocellular carcinoma cells". Molecular Medicine Reports 11.2 (2015): 1455-1461.
Chicago
Xie, Y., Yang, Y., Ji, D., Zhang, D., Yao, X., Zhang, X."Hiwi downregulation, mediated by shRNA, reduces the proliferation and migration of human hepatocellular carcinoma cells". Molecular Medicine Reports 11, no. 2 (2015): 1455-1461. https://doi.org/10.3892/mmr.2014.2847
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