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Effect of immunosuppression on the human mesangial cell cycle

  • Authors:
    • Xiaoshuang Zhou
    • Biruh Workeneh
    • Zhaoyong Hu
    • Rongshan Li
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Provincial People's Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Department of Nephrology, Baylor College of Medicine, Houston, TX 77030, USA
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 910-916
    |
    Published online on: November 4, 2014
       https://doi.org/10.3892/mmr.2014.2861
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Abstract

The present study investigated the effects of immunosuppressive agents [tacrolimus (Tac), cyclosporine A (CsA), mycophenolic acid (MMF) and methylprednisone (MP)] on the proliferation, cell cycle progression and apoptotic rate of human mesangial cells. Cultured human mesangial cells were treated with several concentrations of the immunosuppressive agents for 24, 48 or 72 h. Cell cycle progression, proliferation and apoptosis were analyzed using an MTT assay and flow cytometry. Tac and CsA significantly inhibited the proliferation of human mesangial cells in a dose- and time-dependent manner. Cell cycle analysis revealed that Tac and CsA arrested mesangial cells in the G0/G1 phase, preventing them from entering S phase. Similarly, MP inhibited human mesangial cell growth by causing cell cycle arrest in G0/G1 phase. MMF also inhibited mesangial cell proliferation, but accomplished this by preventing progression from S phase to the G2/M phase. The combination of MP and MMF synergistically inhibited mesangial cell proliferation. Tac, CsA, MP and MMF inhibited proliferation of human mesangial cells by blocking progression of the cell cycle. In conclusion, these agents, sequentially or in combination, may be used to effectively treat mesangial proliferative glomerular disease.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou X, Workeneh B, Hu Z and Li R: Effect of immunosuppression on the human mesangial cell cycle. Mol Med Rep 11: 910-916, 2015.
APA
Zhou, X., Workeneh, B., Hu, Z., & Li, R. (2015). Effect of immunosuppression on the human mesangial cell cycle. Molecular Medicine Reports, 11, 910-916. https://doi.org/10.3892/mmr.2014.2861
MLA
Zhou, X., Workeneh, B., Hu, Z., Li, R."Effect of immunosuppression on the human mesangial cell cycle". Molecular Medicine Reports 11.2 (2015): 910-916.
Chicago
Zhou, X., Workeneh, B., Hu, Z., Li, R."Effect of immunosuppression on the human mesangial cell cycle". Molecular Medicine Reports 11, no. 2 (2015): 910-916. https://doi.org/10.3892/mmr.2014.2861
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou X, Workeneh B, Hu Z and Li R: Effect of immunosuppression on the human mesangial cell cycle. Mol Med Rep 11: 910-916, 2015.
APA
Zhou, X., Workeneh, B., Hu, Z., & Li, R. (2015). Effect of immunosuppression on the human mesangial cell cycle. Molecular Medicine Reports, 11, 910-916. https://doi.org/10.3892/mmr.2014.2861
MLA
Zhou, X., Workeneh, B., Hu, Z., Li, R."Effect of immunosuppression on the human mesangial cell cycle". Molecular Medicine Reports 11.2 (2015): 910-916.
Chicago
Zhou, X., Workeneh, B., Hu, Z., Li, R."Effect of immunosuppression on the human mesangial cell cycle". Molecular Medicine Reports 11, no. 2 (2015): 910-916. https://doi.org/10.3892/mmr.2014.2861
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