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Article

Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor growth

Corrigendum in: /mmr/11/5/4010
  • Authors:
    • Hui-Yong Zhang
    • Bing-Lin Fan
    • Xin-Sheng Wu
    • Ling‑Min  Mu
    • Wen-Feng Wang
    • Wu-Ling Zhu
  • View Affiliations / Copyright

    Affiliations: College of Life Science and Biotechnology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Vasculocardiology, Xinxiang 371 Central Hospital, Xinxiang, Henan 453003, P.R. China
  • Pages: 1483-1489
    |
    Published online on: November 5, 2014
       https://doi.org/10.3892/mmr.2014.2866
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Abstract

A chimeric plasmin‑resistant vascular endothelial growth factor (VEGF)165/VEGF192 (132-158) protein, named as VEGF192 (according to the nomenclature of VEGF), designed by a previous study, was demonstrated to have an enhanced affinity for the extracellular matrix (ECM) amongst other bioactivities. However, it is now accepted that mutant VEGFs frequently demonstrate different angiogenic activities and produce different vascular patterning from the parental molecule. The present study hypothesized that VEGF192, due to its enhanced binding affinity to the ECM, would exhibit a different angiogenic activity and produce a different vascular patterning compared to those of VEGF165. Murine breast cancer EMT‑6 cells were manipulated to stably overexpress VEGF165 or VEGF192. These cells were then inoculated intradermally into BALB/c mice in order to monitor the formation of vascular patterning in skin proximal to tumors. In vivo angiogenesis experiments revealed that overexpression of VEGF192 in murine breast cancer cells resulted in irregular, disorganized and dense vascular patterning as well as induced a significant inhibition of tumor growth compared with that of VEGF165. In addition, allograft tumor immunochemical assays of VEGF192‑overexpressing tumors demonstrated significantly lower vascular densities than those of VEGF165‑overexpressing tumors; however, VEGF192 tumors had a significantly enlarged vascular caliber. Conversely, cell wound healing experiments revealed that VEGF192‑overexpressing EMT‑6 cells had significantly decreased migration rates compared with those of VEGF165‑overexpressing EMT‑6 cells. In conclusion, the results of the present study supported the hypothesis that the altered ECM affinity of VEGF induced structural alterations to vasculature. In addition, these results provided a novel insight into VEGF design and indirect evidence for the function of exon 8 in VEGF.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Fan B, Wu X, Mu LM, Wang W and Zhu W: Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor growth Corrigendum in /mmr/11/5/4010. Mol Med Rep 11: 1483-1489, 2015.
APA
Zhang, H., Fan, B., Wu, X., Mu, L., Wang, W., & Zhu, W. (2015). Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor growth Corrigendum in /mmr/11/5/4010. Molecular Medicine Reports, 11, 1483-1489. https://doi.org/10.3892/mmr.2014.2866
MLA
Zhang, H., Fan, B., Wu, X., Mu, L., Wang, W., Zhu, W."Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor growth Corrigendum in /mmr/11/5/4010". Molecular Medicine Reports 11.2 (2015): 1483-1489.
Chicago
Zhang, H., Fan, B., Wu, X., Mu, L., Wang, W., Zhu, W."Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor growth Corrigendum in /mmr/11/5/4010". Molecular Medicine Reports 11, no. 2 (2015): 1483-1489. https://doi.org/10.3892/mmr.2014.2866
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Fan B, Wu X, Mu LM, Wang W and Zhu W: Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor growth Corrigendum in /mmr/11/5/4010. Mol Med Rep 11: 1483-1489, 2015.
APA
Zhang, H., Fan, B., Wu, X., Mu, L., Wang, W., & Zhu, W. (2015). Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor growth Corrigendum in /mmr/11/5/4010. Molecular Medicine Reports, 11, 1483-1489. https://doi.org/10.3892/mmr.2014.2866
MLA
Zhang, H., Fan, B., Wu, X., Mu, L., Wang, W., Zhu, W."Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor growth Corrigendum in /mmr/11/5/4010". Molecular Medicine Reports 11.2 (2015): 1483-1489.
Chicago
Zhang, H., Fan, B., Wu, X., Mu, L., Wang, W., Zhu, W."Overexpression of the chimeric plasmin-resistant VEGF165/VEGF183 (132-158) protein in murine breast cancer induces distinct vascular patterning adjacent to tumors and retarded tumor growth Corrigendum in /mmr/11/5/4010". Molecular Medicine Reports 11, no. 2 (2015): 1483-1489. https://doi.org/10.3892/mmr.2014.2866
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