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Article

Autologous platelet‑rich plasma promotes proliferation and chondrogenic differentiation of adipose‑derived stem cells

  • Authors:
    • Ji Shen
    • Qingfeng Gao
    • Yao Zhang
    • Yaohua He
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
  • Pages: 1298-1303
    |
    Published online on: November 5, 2014
       https://doi.org/10.3892/mmr.2014.2875
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Abstract

Cartilage regeneration is a promising potential therapy for articular cartilage defects and adult stem cells serve a key role in regenerative medicine. Adipose‑derived stem cells (ADSCs) have been identified as an alternative source of adult stem cells in recent years and can be differentiated into numerous types of cell, including chondrocytes, adipocytes and osteoblasts. However, their clinical use is restricted by the proliferation of cells, and their tendency to dedifferentiate. Platelet‑rich plasma (PRP) has recently emerged as a potential bioactive material to promote cell proliferation and differentiation, based on the release of growth factors. In the current study, the effect of autologous PRP on the proliferation and chondrogenic differentiation of ADSCs was examined. The results indicated that PRP promotes ADSC proliferation and suggested that PRP leads to chondrogenic differentiation of ADSCs in vitro. When co‑cultured with chondrocytes, the ADSCs on three‑dimensional PRP scaffolds were able to form neocartilage, with positive staining of safranine O, which indicated the production of glycosaminoglycan, and type II collagen.
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Copy and paste a formatted citation
Spandidos Publications style
Shen J, Gao Q, Zhang Y and He Y: Autologous platelet‑rich plasma promotes proliferation and chondrogenic differentiation of adipose‑derived stem cells. Mol Med Rep 11: 1298-1303, 2015.
APA
Shen, J., Gao, Q., Zhang, Y., & He, Y. (2015). Autologous platelet‑rich plasma promotes proliferation and chondrogenic differentiation of adipose‑derived stem cells. Molecular Medicine Reports, 11, 1298-1303. https://doi.org/10.3892/mmr.2014.2875
MLA
Shen, J., Gao, Q., Zhang, Y., He, Y."Autologous platelet‑rich plasma promotes proliferation and chondrogenic differentiation of adipose‑derived stem cells". Molecular Medicine Reports 11.2 (2015): 1298-1303.
Chicago
Shen, J., Gao, Q., Zhang, Y., He, Y."Autologous platelet‑rich plasma promotes proliferation and chondrogenic differentiation of adipose‑derived stem cells". Molecular Medicine Reports 11, no. 2 (2015): 1298-1303. https://doi.org/10.3892/mmr.2014.2875
Copy and paste a formatted citation
x
Spandidos Publications style
Shen J, Gao Q, Zhang Y and He Y: Autologous platelet‑rich plasma promotes proliferation and chondrogenic differentiation of adipose‑derived stem cells. Mol Med Rep 11: 1298-1303, 2015.
APA
Shen, J., Gao, Q., Zhang, Y., & He, Y. (2015). Autologous platelet‑rich plasma promotes proliferation and chondrogenic differentiation of adipose‑derived stem cells. Molecular Medicine Reports, 11, 1298-1303. https://doi.org/10.3892/mmr.2014.2875
MLA
Shen, J., Gao, Q., Zhang, Y., He, Y."Autologous platelet‑rich plasma promotes proliferation and chondrogenic differentiation of adipose‑derived stem cells". Molecular Medicine Reports 11.2 (2015): 1298-1303.
Chicago
Shen, J., Gao, Q., Zhang, Y., He, Y."Autologous platelet‑rich plasma promotes proliferation and chondrogenic differentiation of adipose‑derived stem cells". Molecular Medicine Reports 11, no. 2 (2015): 1298-1303. https://doi.org/10.3892/mmr.2014.2875
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