Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells

Deng,Ying‑Hu; Li,Li‑Hua; He,Jin; Li,Mei; Zhang,Yu; Wang,Xiu‑Mei; Cui,Fu‑Zhai; Xia,Hong

doi:10.3892/mmr.2014.2876

February-2015 Volume 11 Issue 2

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February-2015 Volume 11 Issue 2

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Article Open Access

Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells

Authors:
- Ying‑Hu Deng
- Li‑Hua Li
- Jin He
- Mei Li
- Yu Zhang
- Xiu‑Mei Wang
- Fu‑Zhai Cui
- Hong Xia
View Affiliations / Copyright

Affiliations: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Spinal Surgery, Hospital of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China, Department of Materials Science and Engineering, Institute of Regenerative and Biomimetic Materials, Tsinghua University, Beijing 100084, P.R. China

Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 975-981
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Published online on: November 6, 2014

https://doi.org/10.3892/mmr.2014.2876
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Abstract

Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

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1	Kumar A, Biebuyck HA and Whitesides GM: Patterning self-assembled monolayers: applications in materials science. Langmuier. 10:1498–1511. 1994. View Article : Google Scholar
2	Ryan D, Parviz BA, Linder V, et al: Patterning multiple aligned self-assembled monolayers using light. Langmuir. 20:9080–8. 2004. View Article : Google Scholar : PubMed/NCBI
3	García AJ, Vega MD and Boettiger D: Modulation of cell proliferation and differentiation through substrate-dependent changes in fibronectin conformation. Mol Biol Cell. 10:785–798. 1999. View Article : Google Scholar : PubMed/NCBI
4	Tziampazis E, Kohn J and Moghe PV: PEG-variant biomaterials as selectively adhesive protein templates: model surfaces for controlled cell adhesion and migration. Biomaterials. 21:511–520. 2000. View Article : Google Scholar : PubMed/NCBI
5	Bonfield TL, Colton E, Marchant RE and Anderson JM: Cytokine and growth factor production by monocytes/macrophages on protein preadsorbed polymers. J Biomed Mater Res. 26:837–850. 1992. View Article : Google Scholar : PubMed/NCBI
6	Shen M and Horbett TA: The effects of surface chemistry and adsorbed proteins on monocyte/macrophage adhesion to chemically modified polystyrene surfaces. J Biomed Mater Res. 57:336–345. 2001. View Article : Google Scholar : PubMed/NCBI
7	Keselowsky BG, Collard DM and García AJ: Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion. J Biomed Mater Res. 66A:247–259. 2003. View Article : Google Scholar
8	Allen LT, Fox EJ, Blute I, et al: Interaction of soft condensed materials with living cells: phenotype/transcriptome correlations for the hydrophobic effect. Proc Natl Acad Sci USA. 100:6331–6336. 2003. View Article : Google Scholar : PubMed/NCBI
9	Michael KE, Vernekar VN, Keselowsky BG, Meredith JC, Latour RA and García AJ: Adsorption-induced conformational changes in fibronectin due to interactions with well-defined surface chemistries. Langmuir. 19:8033–8040. 2003. View Article : Google Scholar
10	Curran JM, Chen R and Hunt JA: Controlling the phenotype and function of mesenchymal stem cells in vitro by adhesion to silane-modified clean glass surfaces. Biomaterials. 26:7057–7067. 2005. View Article : Google Scholar : PubMed/NCBI
11	Yan H, Zhang S, He J, et al: Self-assembled monolayers with different chemical group substrates for the study of MCF-7 breast cancer cell line behavior. Biomed Mater. 8:0350082013. View Article : Google Scholar : PubMed/NCBI
12	Yu XL, Xu SJ, Shao JD, et al: Different fate of cancer cells on several chemical functional groups. Surface Coating Technol. 228(Suppl 1): S48–S54. 2013. View Article : Google Scholar
13	Picci P: Osteosarcoma (osteogenic sarcoma). Orphanet J Rare Dis. 2:62007. View Article : Google Scholar : PubMed/NCBI
14	Heare T, Hensley MA and Dell’Orfano S: Bone tumors: osteosarcoma and Ewing’s sarcoma. Curr Opin Pediatr. 21:365–372. 2009. View Article : Google Scholar : PubMed/NCBI
15	Li LH, Deng YH, He J, et al: Influence of surface chemistry on the biological feature of giant cell tumor of bone stromal cells in vitro. J Biomater Tissue Eng. 3:554–563. 2013. View Article : Google Scholar
16	Widrig CA, Alves CA and Porter MD: Scanning tunneling microscopy of ethanethiolate and n-octadecanethiolate monolayers spontaneously absorbed at gold surfaces. J Am Chem Soc. 113:2805–2810. 1991. View Article : Google Scholar
17	Lappalainen K, Jääskeläinen I, Syrjänen K, Urtti A and Syrjänen S: Comparison of cell proliferation and toxicity assays using two cationic liposomes. Pharm Res. 11:1127–1131. 1994. View Article : Google Scholar : PubMed/NCBI
18	Green DR and Reed JC: Mitochondria and apoptosis. Science. 281:1309–1312. 1998. View Article : Google Scholar : PubMed/NCBI
19	Keselowsky BG, Collard DM and García AJ: Integrin binding specificity regulates biomaterial surface chemistry effects on cell differentiation. Proc Natl Acad Sci USA. 102:5953–5957. 2005. View Article : Google Scholar : PubMed/NCBI
20	Lincks J, Boyan BD, Blanchard CR, et al: Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition. Biomaterials. 23:2219–2232. 1998. View Article : Google Scholar
21	Shin Y and Akao M: Tissue reactions to various percutaneous materials with different surface properties and structures. Artif Organs. 21:995–1001. 1997. View Article : Google Scholar : PubMed/NCBI
22	Zambonin G and Grano M: Biomaterials in orthopaedic surgery: effects of different hydroxyapatites and demineralized bone matrix on proliferation rate and bone matrix synthesis by human osteoblasts. Biomaterials. 16:397–402. 1995. View Article : Google Scholar : PubMed/NCBI
23	Lobner D: Comparison of the LDH and MTT for quantifying cell death: validity for neuronal apoptosis? J Neurosci Methods. 96:147–152. 2000. View Article : Google Scholar : PubMed/NCBI
24	Wu J: Apoptosis and angiogenesis: two promising tumor markers in breast cancer (review). Anticancer Res. 16(4B): 2233–2239. 1996.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells

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