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Article

Differentiation of human amniotic epithelial cells into Schwann‑like cells via indirect co‑culture with Schwann cells in vitro

  • Authors:
    • Shuang Zhu
    • Jiachun Li
    • Qingtang Zhu
    • Ting Dai
    • Bo  He
    • Xiang  Zhou
    • Jianping Xiang
    • Xiaolin Liu
  • View Affiliations / Copyright

    Affiliations: Department of Microsurgery and Hand Surgery, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Biotechnology, School of Basic Science, Guangzhou Medical University, Guangzhou, Guangdong 510080, P.R. China
  • Pages: 1221-1227
    |
    Published online on: November 6, 2014
       https://doi.org/10.3892/mmr.2014.2881
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Abstract

Human amniotic epithelial cells (hAECs) exhibit multi‑lineage differentiation ability. The present study investigated the possibility that hAECs possess the potential to differentiate into Schwann‑like cells using an in vitro indirect co‑culture approach. hAECs were isolated via enzymatic digestion, and immunocytochemistry and flow cytometry were performed to identify the hAECs. The hAECs were co‑cultured with Schwann cells (SCs) to differentiate the hAECs into Schwann‑like cells via induced proximity. The expression of typical S‑100 SC markers in the co‑cultured hAECs was determined via immunocytochemistry. For the functional experiments, reverse transcription quantitative polymerase chain reaction (RT‑qPCR) was performed to measure the expression levels of nerve growth factor (NGF), brain‑derived neurotrophic factor (BDNF) and glial cell‑derived neurotrophic factor (GDNF) mRNA. In addition, neurite outgrowth was measured in PC12 cells following co‑culture with the differentiated hAECs. Subsequent to co‑culture with SCs for 21 days, the hAECs exhibited spindle‑like morphology. The immunocytochemistry results revealed that the co‑cultured hAECs expressed S‑100, indicating differentiation into Schwann‑like cells. RT‑qPCR revealed that NGF, BDNF and GDNF expression was upregulated upon differentiation. The average axon length of the PC12 cells increased from 21.32±5.45 to 51.32±8.56 µm subsequent to co‑culture with the differentiated hAECs. These results demonstrate that this indirect co‑culture microenvironment induced the hAECs to differentiate into Schwann‑like cells that exhibited the morphological, phenotypic and functional characteristics of SCs. Therefore, the use of differentiated hAECs that exhibit the characteristics of SCs provides a promising alternative to the present techniques used for peripheral nerve regeneration.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu S, Li J, Zhu Q, Dai T, He B, Zhou X, Xiang J and Liu X: Differentiation of human amniotic epithelial cells into Schwann‑like cells via indirect co‑culture with Schwann cells in vitro. Mol Med Rep 11: 1221-1227, 2015.
APA
Zhu, S., Li, J., Zhu, Q., Dai, T., He, B., Zhou, X. ... Liu, X. (2015). Differentiation of human amniotic epithelial cells into Schwann‑like cells via indirect co‑culture with Schwann cells in vitro. Molecular Medicine Reports, 11, 1221-1227. https://doi.org/10.3892/mmr.2014.2881
MLA
Zhu, S., Li, J., Zhu, Q., Dai, T., He, B., Zhou, X., Xiang, J., Liu, X."Differentiation of human amniotic epithelial cells into Schwann‑like cells via indirect co‑culture with Schwann cells in vitro". Molecular Medicine Reports 11.2 (2015): 1221-1227.
Chicago
Zhu, S., Li, J., Zhu, Q., Dai, T., He, B., Zhou, X., Xiang, J., Liu, X."Differentiation of human amniotic epithelial cells into Schwann‑like cells via indirect co‑culture with Schwann cells in vitro". Molecular Medicine Reports 11, no. 2 (2015): 1221-1227. https://doi.org/10.3892/mmr.2014.2881
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu S, Li J, Zhu Q, Dai T, He B, Zhou X, Xiang J and Liu X: Differentiation of human amniotic epithelial cells into Schwann‑like cells via indirect co‑culture with Schwann cells in vitro. Mol Med Rep 11: 1221-1227, 2015.
APA
Zhu, S., Li, J., Zhu, Q., Dai, T., He, B., Zhou, X. ... Liu, X. (2015). Differentiation of human amniotic epithelial cells into Schwann‑like cells via indirect co‑culture with Schwann cells in vitro. Molecular Medicine Reports, 11, 1221-1227. https://doi.org/10.3892/mmr.2014.2881
MLA
Zhu, S., Li, J., Zhu, Q., Dai, T., He, B., Zhou, X., Xiang, J., Liu, X."Differentiation of human amniotic epithelial cells into Schwann‑like cells via indirect co‑culture with Schwann cells in vitro". Molecular Medicine Reports 11.2 (2015): 1221-1227.
Chicago
Zhu, S., Li, J., Zhu, Q., Dai, T., He, B., Zhou, X., Xiang, J., Liu, X."Differentiation of human amniotic epithelial cells into Schwann‑like cells via indirect co‑culture with Schwann cells in vitro". Molecular Medicine Reports 11, no. 2 (2015): 1221-1227. https://doi.org/10.3892/mmr.2014.2881
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