Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
February-2015 Volume 11 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2015 Volume 11 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Synergetic effects of hBMSCs and hPCs in osteogenic differentiation and their capacity in the repair of critical-sized femoral condyle defects

  • Authors:
    • Daoyun Chen
    • Hao Shen
    • Yaohua He
    • Yunsu Chen
    • Qi Wang
    • Jianxi Lu
    • Yao Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, P.R. China, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University, Shanghai 200011, P.R. China
  • Pages: 1111-1119
    |
    Published online on: November 6, 2014
       https://doi.org/10.3892/mmr.2014.2883
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Tissue-engineered bone grafts require an osteoblastic cellular source to be utilized in bone transplantation therapy. Human bone marrow stem cells (hBMSCs) and periosteal-derived stem cells (hPCs) are the commonly used cellular sources for bone tissue engineering and are essential in fracture healing. In the present study, hBMSCs and hPCs were co-cultured from the same donors, as the cellular source. In monolayer cultivation, co-culturing hBMSCs and hPCs demonstrated more robust mineralized nodule formation and stronger alkaline phosphatase (ALP) positive staining than hBMSCs or hPCs. Three-dimensional (3-D) culturing on porous β-tricalcium phosphate (TCP) scaffolds and co-culturing of hBMSCs and hPCs significantly promoted the osteogenic specific mRNA expression of COL1α1, BMP-2, osteopontin (OPN) and osteocalcin (OC). For in vivo bone formation and neovascularization assessment, the cellular-β-TCP scaffolds were transplanted into critical-sized femoral condyle defects in rabbits. The results confirmed that co-culturing hBMSCs and hPCs accelerated bone regeneration and enhanced mature bone formation, but also facilitated central vascularization in scaffold pores. Based on these data, we recommend co-culturing hBMSCs and hPCs as a promising cellular source for bone tissue engineering applications.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Goulet JA, Senunas LE, DeSilva GL and Greenfield ML: Autogenous iliac crest bone graft. Complications and functional assessment. Clin Orthop Relat Res. 76–81. 1997. View Article : Google Scholar : PubMed/NCBI

2 

Ferrara JL and Yanik G: Acute graft versus host disease: pathophysiology, risk factors, and prevention strategies. Clin Adv Hematol Oncol. 3:415–419. 4282005.PubMed/NCBI

3 

Lietman SA, Tomford WW, Gebhardt MC, Springfield DS and Mankin HJ: Complications of irradiated allografts in orthopaedic tumor surgery. Clin Orthop Relat Res. 214–217. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Bruder SP, Jaiswal N, Ricalton NS, Mosca JD, Kraus KH and Kadiyala S: Mesenchymal stem cells in osteobiology and applied bone regeneration. Clin Orthop Relat Res. (Suppl): S247–S256. 1998. View Article : Google Scholar

5 

Srouji S, Maurice S and Livne E: Microscopy analysis of bone marrow-derived osteoprogenitor cells cultured on hydrogel 3-D scaffold. Microsc Res Tech. 66:132–138. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Arnsdorf EJ, Jones LM, Carter DR and Jacobs CR: The periosteum as a cellular source for functional tissue engineering. Tissue Eng Part A. 15:2637–2642. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Barachini S, Trombi L, Danti S, et al: Morpho-functional characterization of human mesenchymal stem cells from umbilical cord blood for potential uses in regenerative medicine. Stem Cells Dev. 18:293–305. 2009. View Article : Google Scholar

8 

Campagnoli C, Roberts IA, Kumar S, Bennett PR, Bellantuono I and Fisk NM: Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow. Blood. 98:2396–2402. 2001. View Article : Google Scholar : PubMed/NCBI

9 

De Coppi P, Bartsch G Jr, Siddiqui MM, et al: Isolation of amniotic stem cell lines with potential for therapy. Nat Biotechnol. 25:100–106. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Derubeis AR and Cancedda R: Bone marrow stromal cells (BMSCs) in bone engineering: limitations and recent advances. Ann Biomed Eng. 32:160–165. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Fickert S, Fiedler J and Brenner RE: Identification, quantification and isolation of mesenchymal progenitor cells from osteoarthritic synovium by fluorescence automated cell sorting. Osteoarthritis Cartilage. 11:790–800. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Zuk PA, Zhu M, Mizuno H, et al: Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 7:211–228. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Mueller SM and Glowacki J: Age-related decline in the osteogenic potential of human bone marrow cells cultured in three-dimensional collagen sponges. J Cell Biochem. 82:583–590. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Phinney DG, Kopen G, Righter W, Webster S, Tremain N and Prockop DJ: Donor variation in the growth properties and osteogenic potential of human marrow stromal cells. J Cell Biochem. 75:424–436. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Eyckmans J and Luyten FP: Species specificity of ectopic bone formation using periosteum-derived mesenchymal progenitor cells. Tissue Eng. 12:2203–2213. 2006. View Article : Google Scholar : PubMed/NCBI

16 

McDuffee LA and Anderson GI: In vitro comparison of equine cancellous bone graft donor sites and tibial periosteum as sources of viable osteoprogenitors. Vet Surg. 32:455–463. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Colnot C: Skeletal cell fate decisions within periosteum and bone marrow during bone regeneration. J Bone Miner Res. 24:274–282. 2009. View Article : Google Scholar

18 

Solchaga LA, Cassiède P and Caplan AI: Different response to osteo-inductive agents in bone marrow- and periosteum-derived cell preparations. Acta Orthop Scand. 69:426–432. 1998. View Article : Google Scholar : PubMed/NCBI

19 

Guichet JM, Braillon P, Bodenreider O and Lascombes P: Periosteum and bone marrow in bone lengthening: a DEXA quantitative evaluation in rabbits. Acta Orthop Scand. 69:527–531. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Huang YC, Kaigler D, Rice KG, Krebsbach PH and Mooney DJ: Combined angiogenic and osteogenic factor delivery enhances bone marrow stromal cell-driven bone regeneration. J Bone Miner Res. 20:848–857. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Jaquiéry C, Schaeren S, Farhadi J, et al: In vitro osteogenic differentiation and in vivo bone-forming capacity of human isogenic jaw periosteal cells and bone marrow stromal cells. Ann Surg. 242:859–867. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Giavaresi G, Fini M, Salvage J, et al: Bone regeneration potential of a soybean-based filler: experimental study in a rabbit cancellous bone defects. J Mater Sci Mater Med. 21:615–626. 2010. View Article : Google Scholar

23 

Mistry AS and Mikos AG: Tissue engineering strategies for bone regeneration. Adv Biochem Eng Biotechnol. 94:1–22. 2005.PubMed/NCBI

24 

Otto WR and Rao J: Tomorrow’s skeleton staff: mesenchymal stem cells and the repair of bone and cartilage. Cell Prolif. 37:97–110. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Salgado AJ, Coutinho OP and Reis RL: Bone tissue engineering: state of the art and future trends. Macromol Biosci. 4:743–765. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Igarashi M, Kamiya N, Hasegawa M, Kasuya T, Takahashi T and Takag M: Inductive effects of dexamethasone on the gene expression of Cbfa1, Osterix and bone matrix proteins during differentiation of cultured primary rat osteoblasts. J Mol Histol. 35:3–10. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Park BW, Hah YS, Kim DR, Kim JR and Byun JH: Osteogenic phenotypes and mineralization of cultured human periosteal-derived cells. Arch Oral Biol. 52:983–989. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Wang J, Asou Y, Sekiya I, Sotome S, Orii H and Shinomiya K: Enhancement of tissue engineered bone formation by a low pressure system improving cell seeding and medium perfusion into a porous scaffold. Biomaterials. 27:2738–2746. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Weinreb M, Shinar D and Rodan GA: Different pattern of alkaline phosphatase, osteopontin, and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res. 5:831–842. 1990. View Article : Google Scholar : PubMed/NCBI

30 

Marom R, Shur I, Solomon R and Benayahu D: Characterization of adhesion and differentiation markers of osteogenic marrow stromal cells. J Cell Physiol. 202:41–48. 2005. View Article : Google Scholar

31 

Stucki U, Schmid J, Hämmerle CF and Lang NP: Temporal and local appearance of alkaline phosphatase activity in early stages of guided bone regeneration. A descriptive histochemical study in humans. Clin Oral Implants Res. 12:121–127. 2001. View Article : Google Scholar : PubMed/NCBI

32 

Wang H, Li Y, Zuo Y, Li J, Ma S and Cheng L: Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/polyamide composite scaffolds for bone tissue engineering. Biomaterials. 28:3338–3348. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Park BW, Hah YS, Kim DR, Kim JR and Byun JH: Vascular endothelial growth factor expression in cultured periosteal-derived cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 105:554–560. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Yuan J, Cui L, Zhang WJ, Liu W and Cao Y: Repair of canine mandibular bone defects with bone marrow stromal cells and porous beta-tricalcium phosphate. Biomaterials. 28:1005–1013. 2007. View Article : Google Scholar

35 

Rai B, Oest ME, Dupont KM, Ho KH, Teoh SH and Guldberg RE: Combination of platelet-rich plasma with polycaprolactone-tricalcium phosphate scaffolds for segmental bone defect repair. J Biomed Mater Res A. 81:888–899. 2007. View Article : Google Scholar : PubMed/NCBI

36 

Marino G, Rosso F, Cafiero G, Tortora C, Moraci M, Barbarisi M and Barbarisi A: Beta-tricalcium phosphate 3-D scaffold promote alone osteogenic differentiation of human adipose stem cells: in vitro study. J Mater Sci Mater Med. 21:353–363. 2010. View Article : Google Scholar

37 

Neamat A, Gawish A and Gamal-Eldeen AM: beta-Tricalcium phosphate promotes cell proliferation, osteogenesis and bone regeneration in intrabony defects in dogs. Arch Oral Biol. 54:1083–1090. 2009. View Article : Google Scholar : PubMed/NCBI

38 

Zheng YX, Ringe J, Liang Z, Loch A, Chen L and Sittinger M: Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum. J Zhejiang Univ Sci B. 7:817–824. 2006. View Article : Google Scholar : PubMed/NCBI

39 

Ignatius A, Blessing H, Liedert A, et al: Tissue engineering of bone: effects of mechanical strain on osteoblastic cells in type I collagen matrices. Biomaterials. 26:311–318. 2005. View Article : Google Scholar

40 

Bilkay U, Tokat C, Helvaci E, Ozek C, Zekioglu O, Onat T and Songur E: Osteogenic capacities of tibial and cranial periosteum: a biochemical and histologic study. J Craniofac Surg. 19:453–458. 2008. View Article : Google Scholar : PubMed/NCBI

41 

Stein GS, Lian JB, Gerstenfeld LG, Shalhoub V, Aronow M, Owen T and Markose E: The onset and progression of osteoblast differentiation is functionally related to cellular proliferation. Connect Tissue Res. 20:3–13. 1989. View Article : Google Scholar : PubMed/NCBI

42 

Giachelli CM and Steitz S: Osteopontin: a versatile regulator of inflammation and biomineralization. Matrix Biol. 19:615–622. 2000. View Article : Google Scholar : PubMed/NCBI

43 

Bouletreau PJ, Warren SM, Spector JA, Peled ZM, Gerrets RP, Greenwald JA and Longaker MT: Hypoxia and VEGF up-regulate BMP-2 mRNA and protein expression in microvascular endothelial cells: implications for fracture healing. Plast Reconstr Surg. 109:2384–2397. 2002. View Article : Google Scholar : PubMed/NCBI

44 

Liang G, Yang Y, Oh S, et al: Ectopic osteoinduction and early degradation of recombinant human bone morphogenetic protein-2-loaded porous beta-tricalcium phosphate in mice. Biomaterials. 26:4265–4271. 2005. View Article : Google Scholar : PubMed/NCBI

45 

Wozney JM: The bone morphogenetic protein family and osteogenesis. Mol Reprod Dev. 32:160–167. 1992. View Article : Google Scholar : PubMed/NCBI

46 

Dufourcq P, Descamps B, Tojais NF, et al: Secreted frizzled-related protein-1 enhances mesenchymal stem cell function in angiogenesis and contributes to neovessel maturation. Stem Cells. 26:2991–3001. 2008. View Article : Google Scholar : PubMed/NCBI

47 

Karageorgiou V and Kaplan D: Porosity of 3-D biomaterial scaffolds and osteogenesis. Biomaterials. 26:5474–5491. 2005. View Article : Google Scholar : PubMed/NCBI

48 

Zhou J, Lin H, Fang T, Li X, Dai W, Uemura T and Dong J: The repair of large segmental bone defects in the rabbit with vascularized tissue engineered bone. Biomaterials. 31:1171–1179. 2010. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen D, Shen H, He Y, Chen Y, Wang Q, Lu J and Jiang Y: Synergetic effects of hBMSCs and hPCs in osteogenic differentiation and their capacity in the repair of critical-sized femoral condyle defects. Mol Med Rep 11: 1111-1119, 2015.
APA
Chen, D., Shen, H., He, Y., Chen, Y., Wang, Q., Lu, J., & Jiang, Y. (2015). Synergetic effects of hBMSCs and hPCs in osteogenic differentiation and their capacity in the repair of critical-sized femoral condyle defects. Molecular Medicine Reports, 11, 1111-1119. https://doi.org/10.3892/mmr.2014.2883
MLA
Chen, D., Shen, H., He, Y., Chen, Y., Wang, Q., Lu, J., Jiang, Y."Synergetic effects of hBMSCs and hPCs in osteogenic differentiation and their capacity in the repair of critical-sized femoral condyle defects". Molecular Medicine Reports 11.2 (2015): 1111-1119.
Chicago
Chen, D., Shen, H., He, Y., Chen, Y., Wang, Q., Lu, J., Jiang, Y."Synergetic effects of hBMSCs and hPCs in osteogenic differentiation and their capacity in the repair of critical-sized femoral condyle defects". Molecular Medicine Reports 11, no. 2 (2015): 1111-1119. https://doi.org/10.3892/mmr.2014.2883
Copy and paste a formatted citation
x
Spandidos Publications style
Chen D, Shen H, He Y, Chen Y, Wang Q, Lu J and Jiang Y: Synergetic effects of hBMSCs and hPCs in osteogenic differentiation and their capacity in the repair of critical-sized femoral condyle defects. Mol Med Rep 11: 1111-1119, 2015.
APA
Chen, D., Shen, H., He, Y., Chen, Y., Wang, Q., Lu, J., & Jiang, Y. (2015). Synergetic effects of hBMSCs and hPCs in osteogenic differentiation and their capacity in the repair of critical-sized femoral condyle defects. Molecular Medicine Reports, 11, 1111-1119. https://doi.org/10.3892/mmr.2014.2883
MLA
Chen, D., Shen, H., He, Y., Chen, Y., Wang, Q., Lu, J., Jiang, Y."Synergetic effects of hBMSCs and hPCs in osteogenic differentiation and their capacity in the repair of critical-sized femoral condyle defects". Molecular Medicine Reports 11.2 (2015): 1111-1119.
Chicago
Chen, D., Shen, H., He, Y., Chen, Y., Wang, Q., Lu, J., Jiang, Y."Synergetic effects of hBMSCs and hPCs in osteogenic differentiation and their capacity in the repair of critical-sized femoral condyle defects". Molecular Medicine Reports 11, no. 2 (2015): 1111-1119. https://doi.org/10.3892/mmr.2014.2883
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team