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Article

Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high glucose through the phosphoinositide 3‑kinase/Akt and extracellular signal‑regulated kinase 1/2 pathways

  • Authors:
    • Hai‑Feng Chen
    • Shu‑Juan Liu
    • Gang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Fujian Provincial Clinical College, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China, Department of Endocrinology, Fujian Provincial Clinical College, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China
  • Pages: 1504-1508
    |
    Published online on: November 6, 2014
       https://doi.org/10.3892/mmr.2014.2884
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Abstract

In the present study, the effect of the heat shock protein 27 (HSP27) signaling pathway on the proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) induced by high glucose (HG) was investigated. HUVEC proliferation in the indicated conditions was measured by the alamarBlue® assay. Apoptosis in HUVECs cultured with HG was analyzed by an Annexin V‑fluorescein isothiocyanate/propidium iodide apoptosis detection kit. HSP27 activity was evaluated by western blotting with specific phospho‑HSP27 antibody. HUVEC proliferation induced by HG was observed to be reduced by the HSP27 inhibitor quercetin in a concentration‑dependent manner, with a concomitant increase in apoptosis. The phosphorylation of HSP27 induced by HG was blocked by the specific phosphoinositide 3‑kinase (PI3K) inhibitor LY294002 and the specific extracellular signal‑regulated kinase (ERK) 1/2 inhibitor U0126 in a concentration‑dependent manner, with peak inhibition rates of 62.6 and 56.1%, respectively. LY294002 and U0126 also reduced HUVEC proliferation with a concomitant increase in apoptotic rate. In conclusion, HSP27 phosphorylation is important in mediating the proliferation and apoptosis of HUVECs induced by high glucose, and PI3K/Akt and ERK1/2 are important signaling pathways that contribute to HSP27 phosphorylation.
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1 

Chen G, Shen X, Yao J, et al: Ablation of NF-kappaB expression by small interference RNA prevents the dysfunction of human umbilical vein endothelial cells induced by high glucose. Endocrine. 35:63–74. 2009. View Article : Google Scholar

2 

Chen G, Chen YF, Chen HF, et al: The effect of NF-κB pathway on proliferation and apoptosis of human umbilical vein endothelial cells induced by intermittent high glucose. Mol Cell Biochem. 347:127–133. 2011. View Article : Google Scholar

3 

Schisano B, Harte AL, Lois K, et al: GLP-1 analogue, Liraglutide protects human umbilical vein endothelial cells against high glucose induced endoplasmic reticulum stress. Regul Pept. 174:46–52. 2012. View Article : Google Scholar

4 

Sun F, Zhou B, Lin X and Duan L: Proteomic analysis identifies nuclear protein effectors in PKC-delta signaling under high glucose-induced apoptosis in human umbilical vein endothelial cells. Mol Med Rep. 4:865–872. 2011.PubMed/NCBI

5 

Son TW, Yun SP, Yong MS, et al: Netrin-1 protects hypoxia-induced mitochondrial apoptosis through HSP27 expression via DCC- and integrin α6β4-dependent Akt, GSK-3β, and HSF-1 in mesenchymal stem cells. Cell Death Dis. 4:e5632013. View Article : Google Scholar

6 

Arany I, Clark JS, Reed DK, Ember I and Juncos LA: Cisplatin enhances interaction between p66Shc and HSP27: its role in reorganization of the actin cytoskeleton in renal proximal tubule cells. Anticancer Res. 32:4759–4763. 2012.PubMed/NCBI

7 

Grogan PT, Sleder KD, Samadi AK, et al: Cytotoxicity of withaferin A in glioblastomas involves induction of an oxidative stress-mediated heat shock response while altering Akt/mTOR and MAPK signaling pathways. Invest New Drugs. 31:545–557. 2013. View Article : Google Scholar :

8 

McClung HM, Golembieski WA, Schultz CR, et al: Deletion of the SPARC acidic domain or EGF-like module reduces SPARC-induced migration and signaling through p38 MAPKHSP27 in glioma. Carcinogenesis. 33:275–284. 2012. View Article : Google Scholar :

9 

Meng G, Sun Y, Fu W, Guo Z and Xu L: Microcystin-LR induces cytoskeleton system reorganization through hyperphosphorylation of tau and HSP27 via PP2A inhibition and subsequent activation of the p38 MAPK signaling pathway in neuroendocrine (PC12) cells. Toxicology. 290:218–229. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Kim J, Kang D, Sun BK, Kim JH and Song JJ: TRAIL/MEKK4/p38/HSP27/Akt survival network is biphasically modulated by the Src/CIN85/c-Cbl complex. Cell Signal. 125:372–379. 2013. View Article : Google Scholar

11 

Ghosh A, Lai C, McDonald S, et al: HSP27 expression in primary colorectal cancers is dependent on mutation of KRAS and PI3K/AKT activation status and is independent of TP53. Exp Mol Pathol. 94:103–108. 2013. View Article : Google Scholar

12 

Bhargavan B, Fatma N, Chhunchha B, et al: LEDGF gene silencing impairs the tumorigenicity of prostate cancer DU145 cells by abating the expression of Hsp27 and activation of the Akt/ERK signaling pathway. Cell Death Dis. 3:e3162012. View Article : Google Scholar : PubMed/NCBI

13 

Qi S, Xin Y, Qi Z, et al: HSP27 phosphorylation modulates TRAIL-induced activation of Src-Akt/ERK signaling through interaction with β-arrestin2. Cell Signal. 26:594–602. 2014. View Article : Google Scholar

14 

Kim J, Kim SY, Kang S, et al: HSP27 modulates survival signaling networks in cells treated with curcumin and TRAIL. Cell Signal. 24:1444–1452. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Chen NG, Lu CC, Lin YH, et al: Proteomic approaches to study epigallocatechin gallate-provoked apoptosis of TSGH-8301 human urinary bladder carcinoma cells: roles of AKT and heat shock protein 27-modulated intrinsic apoptotic pathways. Oncol Rep. 26:939–947. 2011.PubMed/NCBI

16 

Chen HF, Xie LD and Xu CS: Role of heat shock protein 27 phosphorylation in migration of vascular smooth muscle cells. Mol Cell Biochem. 327:1–6. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Chen HF, Xie LD and Xu CS: The signal transduction pathways of heat shock protein 27 phosphorylation in vascular smooth muscle cells. Mol Cell Biochem. 333:49–56. 2010. View Article : Google Scholar

18 

Ueda K, Nakahara T, Akanuma K, et al: Differential effects of LY294002 and wortmannin on neurons and vascular endothelial cells in the rat retina. Pharmacol Rep. 65:854–862. 2013. View Article : Google Scholar : PubMed/NCBI

19 

You JJ, Yang CH, Yang CM and Chen MS: Cyr61 induces the expression of monocyte chemoattractant protein-1 via the integrin ανβ3, FAK, PI3K/Akt, and NF-κB pathways in retinal vascular endothelial cells. Cell Signal. 26:133–140. 2014. View Article : Google Scholar

20 

Chen ML, Lin YH, Yang CM and Hu ML: Lycopene inhibits angiogenesis both in vitro and in vivo by inhibiting MMP-2/uPA system through VEGFR2-mediated PI3K-Akt and ERK/p38 signaling pathways. Mol Nutr Food Res. 56:889–899. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Fournier NM, Lee B, Banasr M, Elsayed M and Duman RS: Vascular endothelial growth factor regulates adult hippocampal cell proliferation through MEK/ERK- and PI3K/Akt-dependent signaling. Neuropharmacology. 63:642–652. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Oviedo-Boyso J, Cortés-Vieyra R, Huante-Mendoza A, et al: The phosphoinositide-3-kinase-Akt signaling pathway is important for Staphylococcus aureus internalization by endothelial cells. Infect Immun. 79:4569–4577. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Shaul YD and Seger R: The MEK/ERK cascade: from signaling specificity to diverse functions. Biochim Biophys Acta. 1773:1213–1226. 2007. View Article : Google Scholar

24 

Will M, Qin AC, Toy W, et al: Rapid induction of apoptosis by PI3K inhibitors is dependent upon their transient inhibition of RAS-ERK signaling. Cancer Discov. 4:334–347. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Wakao K, Watanabe T, Takadama T, et al: Sangivamycin induces apoptosis by suppressing Erk signaling in primary effusion lymphoma cells. Biochem Biophys Res Commun. 444:135–140. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen HF, Liu SJ and Chen G: Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high glucose through the phosphoinositide 3‑kinase/Akt and extracellular signal‑regulated kinase 1/2 pathways. Mol Med Rep 11: 1504-1508, 2015.
APA
Chen, H., Liu, S., & Chen, G. (2015). Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high glucose through the phosphoinositide 3‑kinase/Akt and extracellular signal‑regulated kinase 1/2 pathways. Molecular Medicine Reports, 11, 1504-1508. https://doi.org/10.3892/mmr.2014.2884
MLA
Chen, H., Liu, S., Chen, G."Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high glucose through the phosphoinositide 3‑kinase/Akt and extracellular signal‑regulated kinase 1/2 pathways". Molecular Medicine Reports 11.2 (2015): 1504-1508.
Chicago
Chen, H., Liu, S., Chen, G."Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high glucose through the phosphoinositide 3‑kinase/Akt and extracellular signal‑regulated kinase 1/2 pathways". Molecular Medicine Reports 11, no. 2 (2015): 1504-1508. https://doi.org/10.3892/mmr.2014.2884
Copy and paste a formatted citation
x
Spandidos Publications style
Chen HF, Liu SJ and Chen G: Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high glucose through the phosphoinositide 3‑kinase/Akt and extracellular signal‑regulated kinase 1/2 pathways. Mol Med Rep 11: 1504-1508, 2015.
APA
Chen, H., Liu, S., & Chen, G. (2015). Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high glucose through the phosphoinositide 3‑kinase/Akt and extracellular signal‑regulated kinase 1/2 pathways. Molecular Medicine Reports, 11, 1504-1508. https://doi.org/10.3892/mmr.2014.2884
MLA
Chen, H., Liu, S., Chen, G."Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high glucose through the phosphoinositide 3‑kinase/Akt and extracellular signal‑regulated kinase 1/2 pathways". Molecular Medicine Reports 11.2 (2015): 1504-1508.
Chicago
Chen, H., Liu, S., Chen, G."Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high glucose through the phosphoinositide 3‑kinase/Akt and extracellular signal‑regulated kinase 1/2 pathways". Molecular Medicine Reports 11, no. 2 (2015): 1504-1508. https://doi.org/10.3892/mmr.2014.2884
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