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Article

Differential microRNA expression profile comparison between epidermal stem cells and differentiated keratinocytes

  • Authors:
    • Zhifang Song
    • Dewu Liu
    • Yan Peng
    • Jin Li
    • Zhiwei Zhang
    • Pu Ning
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Burn Center, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
  • Pages: 2285-2291
    |
    Published online on: November 6, 2014
       https://doi.org/10.3892/mmr.2014.2886
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Abstract

The aim of the current study was to analyze the differential microRNA (miRNA) expression profiles of human epidermal stem cells (ESCs) and differentiated keratinocytes. Enzyme digestion was used in combination with rapid adhesion to collagen IV to isolate primary human ESCs and differentiated keratinocytes, from which total RNA was extracted. Fluorescence labeling, microarray hybridization and differential expression analyses were performed. Reverse transcription quantitative polymerase chain reaction (RT‑qPCR) was performed to validate the reliability of the microarray results and predict the target genes of the differentially expressed miRNAs. A total of 25 miRNAs, including hsa‑miR‑197‑5p, hsa‑miR‑125b‑5p and hsa‑miR‑376a‑3p, were upregulated, whereas 166 miRNAs, including hsa‑miR‑29b‑3p, hsa‑miR‑203 and hsa‑miR‑34a‑3p, were downregulated in the human ESCs compared with the expression in differentiated keratinocytes. RT‑qPCR results confirmed the upregulation of hsa‑miR‑197‑5p and the downregulation of hsa‑miR‑29b‑3p, which were consistent with the microarray results. miRNA target prediction indicated that the miRNA expression levels correlated with cell proliferation, differentiation, apoptosis and senescence. Expression levels of miRNAs significantly differed between human ESCs and differentiated keratinocytes. This finding may be attributed to their biological characteristics, such as proliferative behavior and differentiation abilities.
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Copy and paste a formatted citation
Spandidos Publications style
Song Z, Liu D, Peng Y, Li J, Zhang Z and Ning P: Differential microRNA expression profile comparison between epidermal stem cells and differentiated keratinocytes. Mol Med Rep 11: 2285-2291, 2015.
APA
Song, Z., Liu, D., Peng, Y., Li, J., Zhang, Z., & Ning, P. (2015). Differential microRNA expression profile comparison between epidermal stem cells and differentiated keratinocytes. Molecular Medicine Reports, 11, 2285-2291. https://doi.org/10.3892/mmr.2014.2886
MLA
Song, Z., Liu, D., Peng, Y., Li, J., Zhang, Z., Ning, P."Differential microRNA expression profile comparison between epidermal stem cells and differentiated keratinocytes". Molecular Medicine Reports 11.3 (2015): 2285-2291.
Chicago
Song, Z., Liu, D., Peng, Y., Li, J., Zhang, Z., Ning, P."Differential microRNA expression profile comparison between epidermal stem cells and differentiated keratinocytes". Molecular Medicine Reports 11, no. 3 (2015): 2285-2291. https://doi.org/10.3892/mmr.2014.2886
Copy and paste a formatted citation
x
Spandidos Publications style
Song Z, Liu D, Peng Y, Li J, Zhang Z and Ning P: Differential microRNA expression profile comparison between epidermal stem cells and differentiated keratinocytes. Mol Med Rep 11: 2285-2291, 2015.
APA
Song, Z., Liu, D., Peng, Y., Li, J., Zhang, Z., & Ning, P. (2015). Differential microRNA expression profile comparison between epidermal stem cells and differentiated keratinocytes. Molecular Medicine Reports, 11, 2285-2291. https://doi.org/10.3892/mmr.2014.2886
MLA
Song, Z., Liu, D., Peng, Y., Li, J., Zhang, Z., Ning, P."Differential microRNA expression profile comparison between epidermal stem cells and differentiated keratinocytes". Molecular Medicine Reports 11.3 (2015): 2285-2291.
Chicago
Song, Z., Liu, D., Peng, Y., Li, J., Zhang, Z., Ning, P."Differential microRNA expression profile comparison between epidermal stem cells and differentiated keratinocytes". Molecular Medicine Reports 11, no. 3 (2015): 2285-2291. https://doi.org/10.3892/mmr.2014.2886
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