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Article

Differential expression of microRNAs and their target genes in non-small-cell lung cancer

  • Authors:
    • Hui-Young Lee
    • Seon-Sook Han
    • Hwanseok Rhee
    • Jung Hoon Park
    • Jae Seung Lee
    • Yeon-Mok Oh
    • Sun Shim Choi
    • Seung-Ho Shin
    • Woo Jin Kim
  • View Affiliations / Copyright

    Affiliations: Department of Internal Medicine, Kangwon National University Hospital, Kangwon National University School of Medicine, Chuncheon-si, Gangwon-do 200-722, Republic of Korea, Macrogen Bioinformatics Center, Macrogen, Seoul 153-023, Republic of Korea, Department of Pulmonary and Critical Care Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea, Department of Medical Biotechnology, College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon-si, Gangwon-do 200-701, Republic of Korea
  • Pages: 2034-2040
    |
    Published online on: November 6, 2014
       https://doi.org/10.3892/mmr.2014.2890
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Abstract

MicroRNAs (miRNAs) are single‑stranded RNA species that constitute a class of non‑coding RNAs, and are emerging as key regulators of gene expression. Since each miRNA is capable of regulating multiple genes, miRNAs are attractive markers for studies of coordinated gene expression. In this study, we investigated miRNA expression profiling using a massively parallel sequencing technique to compare non‑small‑cell lung cancer (NSCLC) tissue and normal lung tissue. Lung cancer tissue and normal lung tissue were obtained from nine NSCLC patients. RNA isolated from these samples was processed using RNA sequencing (RNA Seq) and the HiSeq 2000 system. Differentially expressed miRNAs and mRNAs were analyzed using a t‑test. We selected target pairs that showed a negative correlation among significantly differentially expressed miRNAs and their putative target mRNAs using miRBase Targets. The differences in the expression levels of 222 miRNAs and 1,597 genes were statistically significant, as indicated by an absolute fold change ≥1.5 and P<0.05. miR‑577, miR‑301b, miR‑944, miR‑891a and miR‑615‑3p were generally upregulated, and miR‑338‑3p was generally downregulated. miRNA‑mRNA target pair analysis revealed that 49 miRNAs had 696 target mRNAs. There were significantly differentially expressed miRNAs and mRNAs between lung cancer and normal tissue. Further investigation of miRNAs and their target genes is warranted to better understand NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Lee H, Han S, Rhee H, Park JH, Lee JS, Oh Y, Choi SS, Shin S and Kim WJ: Differential expression of microRNAs and their target genes in non-small-cell lung cancer. Mol Med Rep 11: 2034-2040, 2015.
APA
Lee, H., Han, S., Rhee, H., Park, J.H., Lee, J.S., Oh, Y. ... Kim, W.J. (2015). Differential expression of microRNAs and their target genes in non-small-cell lung cancer. Molecular Medicine Reports, 11, 2034-2040. https://doi.org/10.3892/mmr.2014.2890
MLA
Lee, H., Han, S., Rhee, H., Park, J. H., Lee, J. S., Oh, Y., Choi, S. S., Shin, S., Kim, W. J."Differential expression of microRNAs and their target genes in non-small-cell lung cancer". Molecular Medicine Reports 11.3 (2015): 2034-2040.
Chicago
Lee, H., Han, S., Rhee, H., Park, J. H., Lee, J. S., Oh, Y., Choi, S. S., Shin, S., Kim, W. J."Differential expression of microRNAs and their target genes in non-small-cell lung cancer". Molecular Medicine Reports 11, no. 3 (2015): 2034-2040. https://doi.org/10.3892/mmr.2014.2890
Copy and paste a formatted citation
x
Spandidos Publications style
Lee H, Han S, Rhee H, Park JH, Lee JS, Oh Y, Choi SS, Shin S and Kim WJ: Differential expression of microRNAs and their target genes in non-small-cell lung cancer. Mol Med Rep 11: 2034-2040, 2015.
APA
Lee, H., Han, S., Rhee, H., Park, J.H., Lee, J.S., Oh, Y. ... Kim, W.J. (2015). Differential expression of microRNAs and their target genes in non-small-cell lung cancer. Molecular Medicine Reports, 11, 2034-2040. https://doi.org/10.3892/mmr.2014.2890
MLA
Lee, H., Han, S., Rhee, H., Park, J. H., Lee, J. S., Oh, Y., Choi, S. S., Shin, S., Kim, W. J."Differential expression of microRNAs and their target genes in non-small-cell lung cancer". Molecular Medicine Reports 11.3 (2015): 2034-2040.
Chicago
Lee, H., Han, S., Rhee, H., Park, J. H., Lee, J. S., Oh, Y., Choi, S. S., Shin, S., Kim, W. J."Differential expression of microRNAs and their target genes in non-small-cell lung cancer". Molecular Medicine Reports 11, no. 3 (2015): 2034-2040. https://doi.org/10.3892/mmr.2014.2890
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