Optimizing methods for the study of intravascular lipid metabolism in zebrafish

  • Authors:
    • Kan Chen
    • Chang-Qian Wang
    • Yu-Qi Fan
    • Yu-Shui Xie
    • Zhao-Fang Yin
    • Zuo-Jun Xu
    • Hui-Li Zhang
    • Jia-Tian Cao
    • Zhi-Hua Han
    • Yue Wang
    • Dong-Qiang Song
  • View Affiliations

  • Published online on: November 7, 2014     https://doi.org/10.3892/mmr.2014.2895
  • Pages: 1871-1876
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Abstract

The zebrafish (Danio rerio) is a useful vertebrate model for use in cardiovascular drug discovery. The present study aimed to construct optimized methods for the study of intravascular lipid metabolism of zebrafish. The lipophilic dye, Oil Red O, was used to stain fasting zebrafish one to eight days post-fertilization (dpf) and to stain 7-dpf zebrafish incubated in a breeding system containing 0.1% egg yolk as a high-fat diet (HFD) for 48 h. Three-dpf zebrafish were kept in CholEsteryl boron-dipyrromethene (BODIPY) 542/563 C11 water for 24 h which indicated the efficiency of CholEsteryl BODIPY 542/563 C11 intravascular cholesterol staining. Subsequently, 7-dpf zebrafish were incubated in water containing the fluorescent probe CholEsteryl BODIPY 542/563 C11 and fed a high-cholesterol diet (HCD) for 10 d. Two groups of 7-dpf zebrafish were incubated in regular breeding water and fed with a regular or HCD containing CholEsteryl BODIPY 542/563 C11 for 10 d. Finally, blood lipids of adult zebrafish fed with regular or HFD for seven weeks were measured. Oil Red O was not detected in the blood vessels of 7-8-dpf zebrafish. Increased intravascular lipid levels were detected in 7-dpf zebrafish incubated in 0.1% egg yolk, indicated by Oil Red O staining. Intravascular cholesterol was efficiently stained in 3-dpf zebrafish incubated in breeding water containing CholEsteryl BODIPY 542/563 C11; however, this method was inappropriate for the calculation of intravascular fluorescence intensity in zebrafish >7‑dpf. In spite of this, intra-aortic fluorescence intensity of zebrafish fed a HCD containing CholEsteryl BODIPY 542/563 C11 was significantly higher (P<0.05) than that of those fed a regular diet containing CholEsteryl BODIPY 542/563 C11. The serum total cholesterol and triglyceride levels of adult zebrafish fed a HFD were markedly increased compared to those of the control group (P<0.05). In conclusion, the use of Oil Red O staining and CholEsteryl BODIPY 542/563 C11 may have applications in zebrafish intravascular lipid metabolism research and screens for novel lipid-regulating drugs.
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March-2015
Volume 11 Issue 3

Print ISSN: 1791-2997
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Spandidos Publications style
Chen K, Wang C, Fan Y, Xie Y, Yin Z, Xu Z, Zhang H, Cao J, Han Z, Wang Y, Wang Y, et al: Optimizing methods for the study of intravascular lipid metabolism in zebrafish. Mol Med Rep 11: 1871-1876, 2015.
APA
Chen, K., Wang, C., Fan, Y., Xie, Y., Yin, Z., Xu, Z. ... Song, D. (2015). Optimizing methods for the study of intravascular lipid metabolism in zebrafish. Molecular Medicine Reports, 11, 1871-1876. https://doi.org/10.3892/mmr.2014.2895
MLA
Chen, K., Wang, C., Fan, Y., Xie, Y., Yin, Z., Xu, Z., Zhang, H., Cao, J., Han, Z., Wang, Y., Song, D."Optimizing methods for the study of intravascular lipid metabolism in zebrafish". Molecular Medicine Reports 11.3 (2015): 1871-1876.
Chicago
Chen, K., Wang, C., Fan, Y., Xie, Y., Yin, Z., Xu, Z., Zhang, H., Cao, J., Han, Z., Wang, Y., Song, D."Optimizing methods for the study of intravascular lipid metabolism in zebrafish". Molecular Medicine Reports 11, no. 3 (2015): 1871-1876. https://doi.org/10.3892/mmr.2014.2895