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Article

A preliminary study on the role of the complement regulatory protein, cluster of differentiation 55, in mice with diabetic neuropathic pain

  • Authors:
    • Fachuan Nie
    • Dong Su
    • Ying Shi
    • Jinmei Chen
    • Haihui Wang
    • Wanxiang Qin
    • Yaohua Chen
    • Suxia Wang
    • Lei Li
  • View Affiliations / Copyright

    Affiliations: Department of Pain, The First Affiliated Hospital, Third Military Medical University, Chongqing 400038, P.R. China, Department of Anesthesiology, Women's and Children's Central Hospital, Chengdu, Sichuan 610031, P.R. China
  • Pages: 2076-2082
    |
    Published online on: November 7, 2014
       https://doi.org/10.3892/mmr.2014.2896
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Abstract

The aim of this study was to investigate the role of the complement regulatory protein cluster of differentiation 55 (CD55) in the pathogenesis of diabetic neuropathic pain (DNP). Healthy adult male C57BL/6J mice were intraperitoneally injected with streptozotocin (STZ) in order to induce DNP. Peripheral blood glucose and protein, and the mRNA expression levels of C3 and CD55 in the spinal cord were determined. In addition, the behaviors of these mice were observed. The results showed that STZ‑treated mice displayed the clinical manifestations of diabetes mellitus, and that their peripheral blood glucose was markedly increased. On the 21st and 28th days following the STZ injection, the mechanical pain threshold and thermal pain threshold of the mice were dramatically reduced (P<0.05). |Additionally, 14 days post‑STZ injection, the mRNA expression of C3 in the spinal cord was significantly increased, which continued for 28 days. On the 21st and 28th days, the number of C3 positive cells in the spinal cord was markedly increased. Seven days after the STZ injection, the number of cells positive for CD55 was markedly reduced in the spinal dorsal horn and subsequently remained at a low level. The mRNA expression of CD55 also was significantly reduced (P<0.05) and remained so for 28 days. The reduction in the expression levels of CD55 occurred earlier than the changes in the expression of C3, suggesting that the downregulation of CD55 expression precedes, and has an important role regarding, the activation of C3 in the occurrence and development of DNP.
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Copy and paste a formatted citation
Spandidos Publications style
Nie F, Su D, Shi Y, Chen J, Wang H, Qin W, Chen Y, Wang S and Li L: A preliminary study on the role of the complement regulatory protein, cluster of differentiation 55, in mice with diabetic neuropathic pain. Mol Med Rep 11: 2076-2082, 2015.
APA
Nie, F., Su, D., Shi, Y., Chen, J., Wang, H., Qin, W. ... Li, L. (2015). A preliminary study on the role of the complement regulatory protein, cluster of differentiation 55, in mice with diabetic neuropathic pain. Molecular Medicine Reports, 11, 2076-2082. https://doi.org/10.3892/mmr.2014.2896
MLA
Nie, F., Su, D., Shi, Y., Chen, J., Wang, H., Qin, W., Chen, Y., Wang, S., Li, L."A preliminary study on the role of the complement regulatory protein, cluster of differentiation 55, in mice with diabetic neuropathic pain". Molecular Medicine Reports 11.3 (2015): 2076-2082.
Chicago
Nie, F., Su, D., Shi, Y., Chen, J., Wang, H., Qin, W., Chen, Y., Wang, S., Li, L."A preliminary study on the role of the complement regulatory protein, cluster of differentiation 55, in mice with diabetic neuropathic pain". Molecular Medicine Reports 11, no. 3 (2015): 2076-2082. https://doi.org/10.3892/mmr.2014.2896
Copy and paste a formatted citation
x
Spandidos Publications style
Nie F, Su D, Shi Y, Chen J, Wang H, Qin W, Chen Y, Wang S and Li L: A preliminary study on the role of the complement regulatory protein, cluster of differentiation 55, in mice with diabetic neuropathic pain. Mol Med Rep 11: 2076-2082, 2015.
APA
Nie, F., Su, D., Shi, Y., Chen, J., Wang, H., Qin, W. ... Li, L. (2015). A preliminary study on the role of the complement regulatory protein, cluster of differentiation 55, in mice with diabetic neuropathic pain. Molecular Medicine Reports, 11, 2076-2082. https://doi.org/10.3892/mmr.2014.2896
MLA
Nie, F., Su, D., Shi, Y., Chen, J., Wang, H., Qin, W., Chen, Y., Wang, S., Li, L."A preliminary study on the role of the complement regulatory protein, cluster of differentiation 55, in mice with diabetic neuropathic pain". Molecular Medicine Reports 11.3 (2015): 2076-2082.
Chicago
Nie, F., Su, D., Shi, Y., Chen, J., Wang, H., Qin, W., Chen, Y., Wang, S., Li, L."A preliminary study on the role of the complement regulatory protein, cluster of differentiation 55, in mice with diabetic neuropathic pain". Molecular Medicine Reports 11, no. 3 (2015): 2076-2082. https://doi.org/10.3892/mmr.2014.2896
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