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Article

Axis inhibition protein 2 polymorphisms may be a risk factor for families with isolated oligodontia

  • Authors:
    • Han Qin
    • Jun Cai
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The First People's Hospital of Lianyungang City, Lianyungang, Jiangsu 222002, P.R. China, Department of Anesthesia, The Third People's Hospital of Lianyungang City, Lianyungang, Jiangsu 222006, P.R. China
  • Pages: 1899-1904
    |
    Published online on: November 7, 2014
       https://doi.org/10.3892/mmr.2014.2900
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Abstract

The objective of the present study was to search for Msh homeobox 1 (MSX1), paired box gene 9 (PAX9), ectodysplasin‑A (EDA) and axis inhibition protein 2 (AXIN2) variants in a family with isolated oligodontia and analyse the pathogenesis of mutations that result in oligodontia phenotypes. Members of a single family (but of different descent) with oligodontia and unrelated healthy controls were enrolled in our study. Genomic DNA was isolated from blood samples. Mutation analysis was performed by amplifying MSX1, PAX9, EDA and AXIN2 exons as well as their exon‑intron boundaries and sequencing the products. DNA sequencing of the AXIN2 gene revealed three mutations in the two patients with oligodontia: a homozygotic silent mutation c.1365A>G (p.Pro455=) in exon 3, two c.956+16A>G mutations (II‑1: homozygosis; III‑1: heterozygosis) and c.1200+71A>G (homozygosis) in the intron, which possibly contributed to structural and functional changes in proteins. The heterozygotic mutations c.1365A>G and c.1200+71A>G were identified in the proband's mother (II‑2). No mutations were detected in the MSX1, PAX9 and EDA genes of oligodontia patients. The findings suggest that the c.956+16A>G, c.1365A>G and c.1200+71A>G mutations of AXIN2 may be responsible for the oligodontia phenotype in this family, but these findings require further study.
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Copy and paste a formatted citation
Spandidos Publications style
Qin H and Cai J: Axis inhibition protein 2 polymorphisms may be a risk factor for families with isolated oligodontia. Mol Med Rep 11: 1899-1904, 2015.
APA
Qin, H., & Cai, J. (2015). Axis inhibition protein 2 polymorphisms may be a risk factor for families with isolated oligodontia. Molecular Medicine Reports, 11, 1899-1904. https://doi.org/10.3892/mmr.2014.2900
MLA
Qin, H., Cai, J."Axis inhibition protein 2 polymorphisms may be a risk factor for families with isolated oligodontia". Molecular Medicine Reports 11.3 (2015): 1899-1904.
Chicago
Qin, H., Cai, J."Axis inhibition protein 2 polymorphisms may be a risk factor for families with isolated oligodontia". Molecular Medicine Reports 11, no. 3 (2015): 1899-1904. https://doi.org/10.3892/mmr.2014.2900
Copy and paste a formatted citation
x
Spandidos Publications style
Qin H and Cai J: Axis inhibition protein 2 polymorphisms may be a risk factor for families with isolated oligodontia. Mol Med Rep 11: 1899-1904, 2015.
APA
Qin, H., & Cai, J. (2015). Axis inhibition protein 2 polymorphisms may be a risk factor for families with isolated oligodontia. Molecular Medicine Reports, 11, 1899-1904. https://doi.org/10.3892/mmr.2014.2900
MLA
Qin, H., Cai, J."Axis inhibition protein 2 polymorphisms may be a risk factor for families with isolated oligodontia". Molecular Medicine Reports 11.3 (2015): 1899-1904.
Chicago
Qin, H., Cai, J."Axis inhibition protein 2 polymorphisms may be a risk factor for families with isolated oligodontia". Molecular Medicine Reports 11, no. 3 (2015): 1899-1904. https://doi.org/10.3892/mmr.2014.2900
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