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Article

GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes

  • Authors:
    • Zubo Wu
    • Hua Peng
    • Qing Du
    • Wen Lin
    • Yali Liu
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
  • Pages: 1837-1844
    |
    Published online on: November 7, 2014
       https://doi.org/10.3892/mmr.2014.2901
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Abstract

GYY4137 is a water‑soluble, small molecule hydrogen sulfide (H2S)‑release agent that possesses potent cardioprotective and anti‑inflammatory properties in experimental models. Coxsackie virus B3 (CVB3) infection commonly causes viral myocarditis, which mainly involves immune cell infiltration, eventually resulting in heart failure. In the present study, the effects and underlying mechanisms of GYY4137 treatment of CVB3‑induced myocarditis were investigated. The effects of GYY4137 on CVB3‑induced nuclear factor‑kappa B (NF‑κB) activity were examined by western blotting, immunofluorescence and electrophoretic mobility shift assay. Mitogen‑activated protein kinase (MAPK) signaling protein expression levels were detected by western blotting. Cardiomyocyte damage‑related enzyme activities, such as lactate dehydrogenase (LDH) and creatine kinase MB (CK‑MB), were measured by ELISA, as well as the production of proinflammatory cytokines. The results revealed that GYY4137 suppressed CVB3‑induced secretion of LDH, CK‑MB and pro‑inflammatory cytokines, such as tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6. Furthermore, the activation of NF‑κB and the IκBα degradation induced by CVB3 were also inhibited by GYY4137. Notably, the phosphorylation of p38, ERK1/2 and JNK1/2 induced by CVB3 was also inhibited by GYY4137. In conclusion, the data demonstrate that GYY4137 exerts anti‑inflammatory effects in CVB3‑infected cardiomyocytes. This anti‑inflammatory mechanism may be associated with suppression of NF‑κB and MAPK signaling pathway activation.
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Copy and paste a formatted citation
Spandidos Publications style
Wu Z, Peng H, Du Q, Lin W and Liu Y: GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes. Mol Med Rep 11: 1837-1844, 2015.
APA
Wu, Z., Peng, H., Du, Q., Lin, W., & Liu, Y. (2015). GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes. Molecular Medicine Reports, 11, 1837-1844. https://doi.org/10.3892/mmr.2014.2901
MLA
Wu, Z., Peng, H., Du, Q., Lin, W., Liu, Y."GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes". Molecular Medicine Reports 11.3 (2015): 1837-1844.
Chicago
Wu, Z., Peng, H., Du, Q., Lin, W., Liu, Y."GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes". Molecular Medicine Reports 11, no. 3 (2015): 1837-1844. https://doi.org/10.3892/mmr.2014.2901
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Z, Peng H, Du Q, Lin W and Liu Y: GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes. Mol Med Rep 11: 1837-1844, 2015.
APA
Wu, Z., Peng, H., Du, Q., Lin, W., & Liu, Y. (2015). GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes. Molecular Medicine Reports, 11, 1837-1844. https://doi.org/10.3892/mmr.2014.2901
MLA
Wu, Z., Peng, H., Du, Q., Lin, W., Liu, Y."GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes". Molecular Medicine Reports 11.3 (2015): 1837-1844.
Chicago
Wu, Z., Peng, H., Du, Q., Lin, W., Liu, Y."GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes". Molecular Medicine Reports 11, no. 3 (2015): 1837-1844. https://doi.org/10.3892/mmr.2014.2901
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