Twist‑related protein 1 enhances oral tongue squamous cell carcinoma cell invasion through β‑catenin signaling

Zheng,Lian; Li,Ning; Guo,Feng; Jian,Xin‑Chun; Jiang,Can‑Hua; Yin,Ping; Min,An‑Jie; Huang,Long

doi:10.3892/mmr.2014.2904

March-2015 Volume 11 Issue 3

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March-2015 Volume 11 Issue 3

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Article

Twist‑related protein 1 enhances oral tongue squamous cell carcinoma cell invasion through β‑catenin signaling

Authors:
- Lian Zheng
- Ning Li
- Feng Guo
- Xin‑Chun Jian
- Can‑Hua Jiang
- Ping Yin
- An‑Jie Min
- Long Huang
View Affiliations / Copyright

Affiliations: Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
Pages: 2255-2261
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Published online on: November 7, 2014

https://doi.org/10.3892/mmr.2014.2904
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Abstract

Accumulating evidence suggests that β‑catenin signaling may be involved in oral tongue squamous cell carcinoma (OTSCC) cell invasion. Abnormal activation of twist‑related protein 1 (TWIST1 or TWIST) has been identified in several types of human cancer. A recent study showed that overexpression of TWIST is associated with a poor prognosis in patients with OTSCC and may enhance OTSCC cell invasion. This study investigated the effect of TWIST on β‑catenin signaling in OTSCC cells and its impact on OSTCC cell invasion. Stable overexpression of TWIST, with or without knockdown of β‑catenin, and stable knockdown of TWIST were performed in SCC‑4 and TCA8113 human OTSCC cells. Overexpression of TWIST in SCC‑4 and TCA8113 cells increased β‑catenin signaling luciferase reporter activity, mRNA levels of the β‑catenin signaling target genes, c‑Myc and c‑Jun levels, soluble β‑catenin level, the phosphorylation status of glycogen synthase kinase‑3β (GSK‑3β) at serine 9, matrix metalloproteinase‑2 (MMP‑2) expression and cell invasion. Knockdown of TWIST had the opposite effect. All of these changes, with the exception of phosphorylation of GSK‑3β, were eliminated by stable knockdown of β‑catenin. In addition, the phosphatidylinositol 3‑kinase (PI3K) inhibitor, LY294002 abrogated the enhancing effects of TWIST on mRNA levels of c‑Myc and c‑Jun, soluble β‑catenin levels, MMP‑2 expression, cell invasion and GSK‑3β phosphorylation. In conclusion, the present study demonstrated that TWIST enhances cell invasion and MMP‑2 expression in OTSCC cells through β‑catenin signaling, probably via a PI3K‑dependent mechanism. This study provides novel insights into the molecular mechanisms underlying OTSCC progression.

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1	Choi KK, Kim MJ, Yun PY, Lee JH, Moon HS, Lee TR and Myoung H: Independent prognostic factors of 861 cases of oral squamous cell carcinoma in Korean adults. Oral Oncol. 42:208–217. 2006. View Article : Google Scholar
2	Xing Y, Qi J, Deng S, Wang C, Zhang L and Chen J: Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition. Exp Cell Res. 319:2058–2072. 2013. View Article : Google Scholar : PubMed/NCBI
3	Su HH, Chu ST, Hou YY, Chang KP and Chen CJ: Spindle cell carcinoma of the oral cavity and oropharynx: factors affecting outcome. J Chin Med Assoc. 69:478–483. 2006. View Article : Google Scholar : PubMed/NCBI
4	Entz-Werlé N, Lavaux T, Metzger N, et al: Involvement of MET/TWIST/APC combination or the potential role of ossification factors in pediatric high-grade osteosarcoma oncogenesis. Neoplasia. 9:678–688. 2007. View Article : Google Scholar : PubMed/NCBI
5	Entz-Werlé N, Choquet P, Neuville A, et al: Targeted apc;twist double-mutant mice: a new model of spontaneous osteosarcoma that mimics the human disease. Transl Oncol. 3:344–353. 2010. View Article : Google Scholar : PubMed/NCBI
6	da Silva SD, Alaoui-Jamali MA, Soares FA, et al: TWIST1 is a molecular marker for a poor prognosis in oral cancer and represents a potential therapeutic target. Cancer. 120:352–362. 2014. View Article : Google Scholar
7	Chesire DR and Isaacs WB: Beta-catenin signaling in prostate cancer: an early perspective. Endocr Relat Cancer. 10:537–560. 2003. View Article : Google Scholar
8	Cawthorn WP, Heyd F, Hegyi K and Sethi JK: Tumour necrosis factor-alpha inhibits adipogenesis via a beta-catenin/TCF4(TCF7L2)-dependent pathway. Cell Death Differ. 14:1361–1373. 2007. View Article : Google Scholar : PubMed/NCBI
9	Nusse R: WNT targets. Repression and activation. Trends Genet. 15:1–3. 1999. View Article : Google Scholar : PubMed/NCBI
10	Gan XQ, Wang JY, Xi Y, Wu ZL, Li YP and Li L: Nuclear Dvl, c-Jun, beta-catenin, and TCF form a complex leading to stabilization of beta-catenin-TCF interaction. J Cell Biol. 180:1087–1100. 2008. View Article : Google Scholar : PubMed/NCBI
11	Sun P, Xiong H, Kim TH, Ren B and Zhang Z: Positive inter-regulation between beta-catenin/T cell factor-4 signaling and endothelin-1 signaling potentiates proliferation and survival of prostate cancer cells. Mol Pharmacol. 69:520–531. 2006. View Article : Google Scholar
12	Wang LP, Chen SW, Zhuang SM, Li H and Song M: Galectin-3 accelerates the progression of oral tongue squamous cell carcinoma via a Wnt/β-catenin-dependent pathway. Pathol Oncol Res. 19:461–474. 2013. View Article : Google Scholar : PubMed/NCBI
13	Kawakita A, Yanamoto S, Yamada SI, Naruse T, Takahashi H, Kawasaki G and Umeda M: MicroRNA-21 promotes oral cancer invasion via the Wnt/β-catenin pathway by targeting DKK2. Pathol Oncol Res. Sep 3–2013.(Epub ahead of print).
14	Matsuo N, Shiraha H, Fujikawa T, et al: Twist expression promotes migration and invasion in hepatocellular carcinoma. BMC Cancer. 9:2402009. View Article : Google Scholar : PubMed/NCBI
15	Wu J, Liao Q, He H, Zhong D and Yin K: TWIST interacts with β-catenin signaling on osteosarcoma cell survival against cisplatin. Mol Carcinog. 53:440–446. 2014. View Article : Google Scholar
16	Sharma M, Chuang WW and Sun Z: Phosphatidylinositol 3-kinase/Akt stimulates androgen pathway through GSK3beta inhibition and nuclear beta-catenin accumulation. J Biol Chem. 277:30935–30941. 2002. View Article : Google Scholar : PubMed/NCBI
17	Fan HX, Li HX, Chen D, Gao ZX and Zheng JH: Changes in the expression of MMP2, MMP9, and ColIV in stromal cells in oral squamous tongue cell carcinoma: relationships and prognostic implications. J Exp Clin Cancer Res. 31:902012. View Article : Google Scholar : PubMed/NCBI
18	Li H, Song H, Luo J, Liang J, Zhao S and Su R: Knockdown of glucose-regulated protein 78 decreases the invasion, metalloproteinase expression and ECM degradation in hepatocellular carcinoma cells. J Exp Clin Cancer Res. 31:392012. View Article : Google Scholar : PubMed/NCBI
19	Entz-Werlé N, Stoetzel C, Berard-Marec P, et al: Frequent genomic abnormalities at TWIST in human pediatric osteosarcomas. Int J Cancer. 117:349–355. 2005. View Article : Google Scholar : PubMed/NCBI
20	Xiao W, Jiang M, Li H, Li C, Su R and Huang K: Knockdown of FAK inhibits the invasion and metastasis of Tca-8113 cells in vitro. Mol Med Rep. 8:703–707. 2013.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Twist‑related protein 1 enhances oral tongue squamous cell carcinoma cell invasion through β‑catenin signaling

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