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Resveratrol exerts an anti-apoptotic effect on human bronchial epithelial cells undergoing cigarette smoke exposure

  • Authors:
    • Li Zhang
    • Xialing Guo
    • Wang Xie
    • Yuping Li
    • Miao Ma
    • Ting Yuan
    • Bailing Luo
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Critical Emergency Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410007, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1752-1758
    |
    Published online on: November 11, 2014
       https://doi.org/10.3892/mmr.2014.2925
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Abstract

Cigarette smoke can cause endoplasmic reticulum stress and induce apoptosis, both of which are important pathogenic factors contributing to chronic obstructive pulmonary disease. The aim of the present study was to produce a cigarette smoke extract (CSE)‑induced apoptosis human bronchial epithelial cell (HBEpC) model, to investigate the protective effects of resveratrol (RES). The role of oxygen‑regulated protein 150 (ORP150) in the RES‑induced activation of Sirtuin 1 (SIRT1) was additionally studied. Cultured HBEpCs were initially treated with CSE to induce apoptosis, followed by an incubation either with or without RES. Numerous techniques were used to evaluate the outcomes of the present study, including cell counting kit‑8 assay, quantitative polymerase chain reaction, western blotting, Hoechst 33342 staining and AnnexinV‑PI flow cytometry apoptosis analyses, and gene knockdown. It was identified that 24 h 2% CSE incubation induced apoptosis in HBEpC, accompanied by an overexpression of the apoptosis molecular markers CCAAT‑enhancer‑binding protein homologous protein, caspase 4 and caspase 3. Pre‑treatment of the cells with RES markedly alleviated the severity of apoptosis, as confirmed by apoptosis analyses and the expression levels of the apoptosis molecular markers. SIRT1 was shown to be overexpressed following RES treatment. However, following the gene knockdown of ORP150, the anti‑apoptotic effects of RES were significantly attenuated. The results of the present study demonstrate that RES may have a protective effect against CSE‑induced apoptosis, and a molecular pathway involving SIRT1 and ORP150 may be associated with the anti‑apoptotic functions of RES in HBEpC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Guo X, Xie W, Li Y, Ma M, Yuan T and Luo B: Resveratrol exerts an anti-apoptotic effect on human bronchial epithelial cells undergoing cigarette smoke exposure. Mol Med Rep 11: 1752-1758, 2015.
APA
Zhang, L., Guo, X., Xie, W., Li, Y., Ma, M., Yuan, T., & Luo, B. (2015). Resveratrol exerts an anti-apoptotic effect on human bronchial epithelial cells undergoing cigarette smoke exposure. Molecular Medicine Reports, 11, 1752-1758. https://doi.org/10.3892/mmr.2014.2925
MLA
Zhang, L., Guo, X., Xie, W., Li, Y., Ma, M., Yuan, T., Luo, B."Resveratrol exerts an anti-apoptotic effect on human bronchial epithelial cells undergoing cigarette smoke exposure". Molecular Medicine Reports 11.3 (2015): 1752-1758.
Chicago
Zhang, L., Guo, X., Xie, W., Li, Y., Ma, M., Yuan, T., Luo, B."Resveratrol exerts an anti-apoptotic effect on human bronchial epithelial cells undergoing cigarette smoke exposure". Molecular Medicine Reports 11, no. 3 (2015): 1752-1758. https://doi.org/10.3892/mmr.2014.2925
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Guo X, Xie W, Li Y, Ma M, Yuan T and Luo B: Resveratrol exerts an anti-apoptotic effect on human bronchial epithelial cells undergoing cigarette smoke exposure. Mol Med Rep 11: 1752-1758, 2015.
APA
Zhang, L., Guo, X., Xie, W., Li, Y., Ma, M., Yuan, T., & Luo, B. (2015). Resveratrol exerts an anti-apoptotic effect on human bronchial epithelial cells undergoing cigarette smoke exposure. Molecular Medicine Reports, 11, 1752-1758. https://doi.org/10.3892/mmr.2014.2925
MLA
Zhang, L., Guo, X., Xie, W., Li, Y., Ma, M., Yuan, T., Luo, B."Resveratrol exerts an anti-apoptotic effect on human bronchial epithelial cells undergoing cigarette smoke exposure". Molecular Medicine Reports 11.3 (2015): 1752-1758.
Chicago
Zhang, L., Guo, X., Xie, W., Li, Y., Ma, M., Yuan, T., Luo, B."Resveratrol exerts an anti-apoptotic effect on human bronchial epithelial cells undergoing cigarette smoke exposure". Molecular Medicine Reports 11, no. 3 (2015): 1752-1758. https://doi.org/10.3892/mmr.2014.2925
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