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Article

Expression patterns of dishevelled-2 in different colon tissue segments in Hirschsprung's disease

  • Authors:
    • Dong Chen
    • Jie Mi
    • Dong-Hui Fu
    • Wei-Lin Wang
    • Hong Gao
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Surgery, Shengjing Hospital of China Medical University, Liaoning 110004, P.R. China, Department of Urinary Surgery, Shengjing Hospital of China Medical University, Liaoning 110004, P.R. China
  • Pages: 2092-2096
    |
    Published online on: November 12, 2014
       https://doi.org/10.3892/mmr.2014.2932
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Abstract

Hirschsprung's disease (HSCR) is a congenital disorder characterized by an absence of enteric ganglion cells in the terminal regions of the gut during development. To date, the cause of HSCR remains unclear, although the pathogenesis of this complex disease is hypothesized to be influenced by numerous genetic and environmental factors. Dishevelled‑2 (DVL‑2) is a subtype of the dishevelled protein, which is known to be involved in embryonic development. In the present study, the pathogenesis of HSCR was investigated by measuring the expression of the DVL‑2 gene and protein using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), western blotting and immunohistochemistry staining in the aganglionic and ganglionic segments of colonic tissues in patients with HSCR. The results showed that the level of DVL‑2 mRNA in the aganglionic segments was 0.28 fold that of the ganglionic segments. Similarly, the protein expression of DVL‑2 was lower (11.31±2.23) in the aganglionic segments than that of the ganglionic segments (35.21±2.66), as assessed by western blot analysis. Furthermore, immunohistochemical staining demonstrated that DVL‑2 expression was significantly higher in the mucosal and submucosal layers from ganglionic colon segments compared with that from the aganglionic segments. The data suggest that the expression of DVL‑2 in colon tissue segments may be important in the pathogenesis of HSCR.
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Copy and paste a formatted citation
Spandidos Publications style
Chen D, Mi J, Fu D, Wang W and Gao H: Expression patterns of dishevelled-2 in different colon tissue segments in Hirschsprung's disease. Mol Med Rep 11: 2092-2096, 2015.
APA
Chen, D., Mi, J., Fu, D., Wang, W., & Gao, H. (2015). Expression patterns of dishevelled-2 in different colon tissue segments in Hirschsprung's disease. Molecular Medicine Reports, 11, 2092-2096. https://doi.org/10.3892/mmr.2014.2932
MLA
Chen, D., Mi, J., Fu, D., Wang, W., Gao, H."Expression patterns of dishevelled-2 in different colon tissue segments in Hirschsprung's disease". Molecular Medicine Reports 11.3 (2015): 2092-2096.
Chicago
Chen, D., Mi, J., Fu, D., Wang, W., Gao, H."Expression patterns of dishevelled-2 in different colon tissue segments in Hirschsprung's disease". Molecular Medicine Reports 11, no. 3 (2015): 2092-2096. https://doi.org/10.3892/mmr.2014.2932
Copy and paste a formatted citation
x
Spandidos Publications style
Chen D, Mi J, Fu D, Wang W and Gao H: Expression patterns of dishevelled-2 in different colon tissue segments in Hirschsprung's disease. Mol Med Rep 11: 2092-2096, 2015.
APA
Chen, D., Mi, J., Fu, D., Wang, W., & Gao, H. (2015). Expression patterns of dishevelled-2 in different colon tissue segments in Hirschsprung's disease. Molecular Medicine Reports, 11, 2092-2096. https://doi.org/10.3892/mmr.2014.2932
MLA
Chen, D., Mi, J., Fu, D., Wang, W., Gao, H."Expression patterns of dishevelled-2 in different colon tissue segments in Hirschsprung's disease". Molecular Medicine Reports 11.3 (2015): 2092-2096.
Chicago
Chen, D., Mi, J., Fu, D., Wang, W., Gao, H."Expression patterns of dishevelled-2 in different colon tissue segments in Hirschsprung's disease". Molecular Medicine Reports 11, no. 3 (2015): 2092-2096. https://doi.org/10.3892/mmr.2014.2932
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