Picosecond pulsed electric fields induce apoptosis in a cervical cancer xenograft

Jia,Jia; Xiong,Zheng‑Ai; Qin,Qin; Yao,Chen‑Guo; Zhao,Xiao‑Zhen

doi:10.3892/mmr.2014.2953

March-2015 Volume 11 Issue 3

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March-2015 Volume 11 Issue 3

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Picosecond pulsed electric fields induce apoptosis in a cervical cancer xenograft

Authors:
- Jia Jia
- Zheng‑Ai Xiong
- Qin Qin
- Chen‑Guo Yao
- Xiao‑Zhen Zhao
View Affiliations / Copyright

Affiliations: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China, State Key Laboratory of Power Transmission Equipment and System Security and New Technology, Chongqing University, Chongqing 400044, P.R. China

Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 1623-1628
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Published online on: November 14, 2014

https://doi.org/10.3892/mmr.2014.2953
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Abstract

The aim of the present study was to evaluate the efficacy of picosecond pulsed electric fields (psPEF) on a cervical cancer xenograft. Human cervical cancer xenografts were established in nude mice by transplantation of HeLa cells, and the tumors were then treated with psPEF. The histological changes were observed by hematoxylin‑eosin staining and transmission electron microscopy. The rate of tumor cell apoptosis was determined using a terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling assay. The mitochondrial transmembrane potential of the tumor cells was detected by laser scanning confocal microscopy, and the activity of caspase‑3, ‑8, ‑9 and ‑12 was determined. The inhibitory rate seven days post‑psPEF treatment was also calculated. The results showed that exposure to psPEF led to an increased rate of apoptosis, collapse of mitochondrial transmembrane potential, and activation of caspases. The inhibitory rate was 9.11% at day 7. The results of the present study indicate that psPEF may induce apoptosis in a cervical cancer xenograft through the endoplasmic reticulum stress and caspase‑dependent signaling pathways.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Picosecond pulsed electric fields induce apoptosis in a cervical cancer xenograft

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