Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
March-2015 Volume 11 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2015 Volume 11 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury

  • Authors:
    • Xinhong Guo
    • Wenjiang Cao
    • Jiaming Yao
    • Yong Yuan
    • Ye  Hong
    • Xinchun Wang
    • Jianguo Xing
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, Xinjiang 832000, P.R. China, Xinjiang Institute of Materia Medica, Ürümqi, Xinjiang 830004, P.R. China
  • Pages: 2227-2233
    |
    Published online on: November 14, 2014
       https://doi.org/10.3892/mmr.2014.2954
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Tilianin, the main effective flavonoid monomer enriched from Dracocephalum moldavuca L., has been shown to have cardioprotective effects. However, the mechanism of tilianin cardioprotection remains largely unknown. The present study aimed to investigate the effects of tilianin preconditioning on myocardial ischemia/reperfusion injury and to analyze the possible mechanism of action. A total of 48 male Sprague Dawley rats were randomized into sham, model myocardial ischemia/reperfusion injury (MI/RI), propranolol hydrochloride positive control, and high‑, medium‑ and low‑dose tilianin groups (n=8 each). The rats in the tilianin groups were perfused with either 1.5, 2.5 or 5.0 mg/kg/d tilianin a week prior to surgery. The positive control group were perfused with 25 mg/kg/d propranolol. Saline was administered to the sham surgery and the MI/RI groups. The MI/RI model was established by ligating the left anterior descending coronary artery for 30 min, which was subsequently removed and the mice were observed for 120 min prior to sacrifice. Na+‑K+‑ATPase, Ca2+‑ATPase, nitric oxide (NO), nitric oxide synthase (NOS) and endothelial system‑related factors were analyzed using the respective detection kits. Immunohistochemistry was used to determine the expression levels of Bcl‑2 and Bax. Caspase‑3 activity was measured by quantitative polymerase chain reaction. The results showed that tilianin preconditioning significantly increased ATPase activity (P<0.01 and P<0.05) as compared with the model group. With regards to the regulation of endothelial function, significant decreases (P<0.01 and P<0.05) were detected in the serum NO levels and myocardial NOS activity when tilianin was administered to MI/RI rats, as compared with the model group, . In addition, the tilianin drug groups exhibited dose‑dependent reductions in the serum levels of endothelin 1 and thromboxane B2, and increases in the serum levels of calcitonin gene‑related peptide and 6‑keto prostaglandin F1a as compared with the model group (P<0.01 and P<0.05). Notably, the administration of tilianin significantly inhibited apoptosis, as evidenced by an increase in Bcl‑2 expression, and reductions in Bax and caspase‑3 mRNA expression levels (P<0.01 and P<0.05). These data indicate that pretreatment with tilianin exerts potent cardioprotective effects in rats with MI/RI. The anti‑MI/RI effects comprised relieving calcium overload, correction of energy metabolism, improvement of endothelial function and inhibiting cell apoptosis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Kreatsoulas C and Anand SS: The impact of social determinants on cardiovascular disease. Can J Cardiol. 26:8C–13C. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Hausenloy DJ and Yellon DM: New directions for protecting the heart against ischemia-reperfusion injury: targeting the Reperfusion injury Salvage Kinase (RISK)-pathway. Cardiovasc Res. 61:448–460. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Ambrosio G and Tritto I: Reperfusion injury: experimental evidence and clinical implications. Am Heart J. 138:S69–S75. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Yellon DM and Baxter GF: Protecting the ischemic and reperfused myocardium in acute myocardial infarction: distant dream or near reality? Heart. 83:381–387. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Li J, Zhang H and Zhang C: Role of inflammation in the regulation of coronary blood flow in ischemia and reperfusion: mechanisms and therapeutic implications. J Mol Cell Cardiol. 52:865–872. 2012. View Article : Google Scholar

6 

Gottlieb RA: Cell death pathways in acute ischemia/reperfusion injury. J Cardiovasc Pharmacol Ther. 16:233–238. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Koenitze JR and Freeman BA: Redox signaling in inflammation: interactions of endogenous electrophiles and mitochondria in cardiovascular disease. Ann NY Acad Sci. 12:45–52. 2010. View Article : Google Scholar

8 

Lakshmi SV, Padmaja G, Kuppusamy P and Kutala VK: Oxidative stress in cardiovascular disease. Indian J Biochem Biophys. 46:421–440. 2009.

9 

Liou SF, Ke HJ, Hsu JH, et al: San-Huang-Xie-Xin Tang prevents rat hearts from ischemia/reperfusion-induced apoptosis through eNOS and MAPK pathways. Evid Based Complement Alternat. 2011:9150512011.

10 

Feng Changgen and Li Qiong: The research of Dracocephalum moldavuca L. chemical constituents and pharmacological activities. J Tradit Chin Med. 25:1552003.

11 

Yuan Yong, Xing Jian Guo, Zhang Yong Jun, et al: Determination the content of tilianin in Dracocephalum moldavuca L. by HPLC. Chin J Exp Tradit Med Formulae. 16:68–69. 2010.

12 

Nam KH, Choi JH, Seo YJ, et al: Inhibitory effects of tilianin on the expression of inducible nitric oxide synthase in low density lipoprotein receptor deficiency mice. Exp Mol Med. 38:445–452. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Guo Xinhong, Cao Wenjiang, Fan Xinmei, et al: The protective effects of Tilianin on myocardial ischemia-reperfusion injury in rats. Chin J Exp Tradit Med Formulae. 13:169–172. 2013.

14 

Zhao Q, Shao L, Hu X, Wu G, et al: Lipoxin A4 preconditioning and postconditioning protect myocardial ischemia/reperfusion injury in rats. Mediators Inflamm. 2013:2313512013.

15 

Wei Q, Yin Y, Xi M, et al: Antioxidant properties of magnesium lithospermate B contribute to the cardioprotection against myocardial ischemia/reperfusion injury in vivo and in vitro. J Tradit Chin Med. 33:85–91. 2013. View Article : Google Scholar

16 

Ahmed LA, Salem HA, Attia AS and Agha AM: Pharmacological preconditioning with nicorandil and pioglitazone attenuates myocardial ischemia/reperfusion injury in rats. Eur J Pharmacol. 663:51–58. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Sun C, Liu C, Li S, et al: Overexpression of GEFT, a Rho family guanine nucleotide exchange factor, predicts poor prognosis in patients with rhabdomyosarcoma. Int J Clin Exp Pathol. 7:1606–1615. 2014.PubMed/NCBI

18 

Zhao J, Xue W and Liang S: Protective effects of dracocephalum moldavica extracts on hypoxia/reoxygenation injury of cardiomyocytes in cultured neonatal rat. J Zhenzhou University (Med Sci). 2010:485–487. 2010.

19 

Fan XM, Cao WJ, Xing JG, et al: Study on the protective effect of Dracocephalum total flavones against myocardial ischemia-reperfusion injury in rats. Chin Tradit Patent Med. 8:1625–1629. 2013.(In Chinese).

20 

Rajadurai M and Stanely Mainzen Prince P: Preventive effect of naringin on cardiac markers, electrocardiographic patterns and lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats. Toxicol. 230:178–188. 2007. View Article : Google Scholar

21 

Tian D, Dmitrieva RI, Doris PA, et al: Protein kinase M zeta regulation of Na/K ATPase: a persistent neuroprotective mechanism of ischemic preconditioning in hippocampal slice cultures. Brain Res. 1213:127–139. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Yorozuya T, Adachi N, Dote K, et al: Enhancement of Na+K+-ATPase and Ca2+-ATPase activities in multi-cycle ischemic preconditioning in rabbit hearts. Eur J Cardiothorac Surg. 26:981–987. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Yang G, Fang Z, Liu Y, et al: Protective effects of Chinese traditional medicine Buyang Huanwu decoction on myocardial injury. Evid Based Complement Alternat Med. 2011:9303242011. View Article : Google Scholar :

24 

Razavi HM, Hamilton JA and Feng Q: Modulation of apoptosis by nitric oxide: implications in myocardial ischemia and heart failure. Pharmacol Ther. 106:147–162. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Liu YN, Zhou ZM and Chen P: Evidence that hydroxysafflor yellow A protects the heart against ischaemia-reperfusion injury by inhibiting mitochondrial permeability transition pore opening. Clin Exp Pharmacol Physiol. 35:211–216. 2008.

26 

Benigni A, Perico N and Remuzzi G: Endothelin antagonists and renal protection. J Cardiovasc Pharmacol. 35(4 Suppl 2): S75–S78. 2000. View Article : Google Scholar : PubMed/NCBI

27 

de Hoon JN, Pickkers P, Smits P, et al: Calcitonin gene-related peptide: exploring its vasodilating mechanism of action in humans. Clin Pharmacol Ther. 73:312–321. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Ji L, Fu F, Zhang L, Liu W, Cai X, et al: Insulin attenuates myocardial ischemia/reperfusion injury via reducing oxidative/nitrative stress. Am J Physiol Endocrinol Metab. 298:E871–E880. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Moudgil R, Menon V, Xu Y, et al: Postischemic apoptosis and functional recovery after angiotensin II type 1 receptor blockade inisolated working rat hearts. J Hypertens. 19:1121–1129. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Chen Z, Chua CC, Ho YS, et al: Overexpression of Bcl-2 attenuates apoptosis and protects against myocardial I/R injury in transgenic mice. Am J Physiol Heart Circ Physiol. 280:H2313–H2320. 2001.PubMed/NCBI

31 

Kumar D and Jugdutt BI: Apoptosis and oxidants in the heart. J Lab Clin Med. 142:288–297. 2003. View Article : Google Scholar : PubMed/NCBI

32 

Nishihara M, Miura T, Miki T, et al: Modulation of the mitochondrial permeability transition pore complex in GSK-3beta-mediated myocardial protection. J Mol Cell Cordial. 43:564–570. 2007. View Article : Google Scholar

33 

Zhang Q, Xiang J, Wang X, et al: Beta(2)-adrenoceptor agonist clenbuterol reduces infarct size and myocardial apoptosis after myocardial ischaemia/reperfusion in anaesthetized rats. Br J Pharmacol. 160:1561–1572. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Guo X, Cao W, Yao J, Yuan Y, Hong Y, Wang X and Xing J: Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury. Mol Med Rep 11: 2227-2233, 2015.
APA
Guo, X., Cao, W., Yao, J., Yuan, Y., Hong, Y., Wang, X., & Xing, J. (2015). Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury. Molecular Medicine Reports, 11, 2227-2233. https://doi.org/10.3892/mmr.2014.2954
MLA
Guo, X., Cao, W., Yao, J., Yuan, Y., Hong, Y., Wang, X., Xing, J."Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury". Molecular Medicine Reports 11.3 (2015): 2227-2233.
Chicago
Guo, X., Cao, W., Yao, J., Yuan, Y., Hong, Y., Wang, X., Xing, J."Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury". Molecular Medicine Reports 11, no. 3 (2015): 2227-2233. https://doi.org/10.3892/mmr.2014.2954
Copy and paste a formatted citation
x
Spandidos Publications style
Guo X, Cao W, Yao J, Yuan Y, Hong Y, Wang X and Xing J: Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury. Mol Med Rep 11: 2227-2233, 2015.
APA
Guo, X., Cao, W., Yao, J., Yuan, Y., Hong, Y., Wang, X., & Xing, J. (2015). Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury. Molecular Medicine Reports, 11, 2227-2233. https://doi.org/10.3892/mmr.2014.2954
MLA
Guo, X., Cao, W., Yao, J., Yuan, Y., Hong, Y., Wang, X., Xing, J."Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury". Molecular Medicine Reports 11.3 (2015): 2227-2233.
Chicago
Guo, X., Cao, W., Yao, J., Yuan, Y., Hong, Y., Wang, X., Xing, J."Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury". Molecular Medicine Reports 11, no. 3 (2015): 2227-2233. https://doi.org/10.3892/mmr.2014.2954
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team