Apigenin inhibits the self-renewal capacity of human ovarian cancer SKOV3‑derived sphere-forming cells

  • Authors:
    • Ai‑Qiong Tang
    • Xiao‑Cheng Cao
    • Li Tian
    • Lihua He
    • Fei Liu
  • View Affiliations

  • Published online on: November 18, 2014     https://doi.org/10.3892/mmr.2014.2974
  • Pages: 2221-2226
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Abstract

Casein kinase 2 (CK2) is a protein kinase which is frequently activated in cancer. The Hedgehog (Hh) signaling pathway is involved in the stimulation of cancer stem cell growth. Its aberrant activation has been validated in several types of cancer, including ovarian cancer. In the present study, the sphere‑forming cells (SFCs) of the human ovarian cancer SKOV3 cell line were observed to have self‑renewal capacity, indicating the possession of ovarian cancer stem‑like cell properties. SKOV3‑derived SFCs had higher levels of CK2α and glioma‑associated oncogene 1 (Gli1) proteins compared with those of parental cells. Apigenin, a common flavonoid, significantly inhibited the self‑renewal capacity and the protein expression of CK2α and Gli1 proteins in the SKOV3‑derived SFCs, which occurred in a concentration‑dependent manner. In addition, CK2α small interfering RNA downregulated the protein expression of CK2α and Gli1 and synergistically inhibited the self‑renewal capacity of the SKOV3‑derived SFCs with apigenin. However, forced overexpression of CK2α resulted in an increase in the expression of CK2α and Gli1 and attenuated the apigenin‑inhibited self‑renewal effect in the SKOV3‑derived SFCs. These results suggested that apigenin inhibited the self‑renewal capacity of SKOV3‑derived SFCs and was involved in downregulating the expression of Gli1 by the inhibition of CK2α.
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March-2015
Volume 11 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Tang AQ, Cao XC, Tian L, He L and Liu F: Apigenin inhibits the self-renewal capacity of human ovarian cancer SKOV3‑derived sphere-forming cells. Mol Med Rep 11: 2221-2226, 2015
APA
Tang, A., Cao, X., Tian, L., He, L., & Liu, F. (2015). Apigenin inhibits the self-renewal capacity of human ovarian cancer SKOV3‑derived sphere-forming cells. Molecular Medicine Reports, 11, 2221-2226. https://doi.org/10.3892/mmr.2014.2974
MLA
Tang, A., Cao, X., Tian, L., He, L., Liu, F."Apigenin inhibits the self-renewal capacity of human ovarian cancer SKOV3‑derived sphere-forming cells". Molecular Medicine Reports 11.3 (2015): 2221-2226.
Chicago
Tang, A., Cao, X., Tian, L., He, L., Liu, F."Apigenin inhibits the self-renewal capacity of human ovarian cancer SKOV3‑derived sphere-forming cells". Molecular Medicine Reports 11, no. 3 (2015): 2221-2226. https://doi.org/10.3892/mmr.2014.2974