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Article

5-Aminolevulinic acid-induced protoporphyrin IX with multi-dose ionizing irradiation enhances host antitumor response and strongly inhibits tumor growth in experimental glioma in vivo

  • Authors:
    • Junkoh Yamamoto
    • Shun‑Ichiro Ogura
    • Shohei Shimajiri
    • Yoshiteru Nakano
    • Daisuke Akiba
    • Takehiro Kitagawa
    • Kunihiro Ueta
    • Tohru Tanaka
    • Shigeru Nishizawa
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, University of Occupational and Environmental Health, Kitakyushu, Fukuoka 807‑8555, Japan, Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa 226‑8501, Japan, Department of Surgical Pathology, University of Occupational and Environmental Health, Kitakyushu, Fukuoka 807‑8555, Japan, SBI Pharmaceuticals Co., Ltd., Minato‑ku, Tokyo 106‑6020, Japan
  • Pages: 1813-1819
    |
    Published online on: November 21, 2014
       https://doi.org/10.3892/mmr.2014.2991
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Abstract

Ionizing irradiation is a well‑established therapeutic modality for malignant gliomas. Due to its high cellular uptake, 5‑aminolevulinic acid (ALA) is used for fluorescence‑guided resection of malignant gliomas. We have previously shown that 5‑ALA sensitizes glioma cells to irradiation in vitro. The aim of the present study was to assess whether 5‑ALA acts as a radiosensitizer in experimental glioma in vivo. Rats were subcutaneously injected with 9L gliosarcoma cells and administered 5‑ALA. The accumulation of 5‑ALA‑induced protoporphyrin IX was confirmed by high‑performance liquid chromatography (HPLC) analysis. Subcutaneous (s.c.) tumors were subsequently irradiated with 2 Gy/day for five consecutive days. In the experimental glioma model, high‑performance liquid chromatography analysis revealed a high level of accumulation of 5‑ALA‑induced protoporphyrin IX in s.c. tumors 3 h after 5‑ALA administration. Multi‑dose ionizing irradiation induced greater inhibition of tumor growth in rats that were administered 5‑ALA than in the non‑5‑ALA‑treated animals. Immunohistochemical analysis of the s.c. tumors revealed that numerous ionized calcium‑binding adapter molecule 1 (Iba1)‑positive macrophages gathered at the surface of and within the s.c. tumors following multi‑dose ionizing irradiation in combination with 5‑ALA administration. By contrast, the s.c. tumors in the control group scarcely showed aggregation of Iba1‑positive macrophages. These results suggested that multi‑dose ionizing irradiation with 5‑ALA induced not only a direct cytotoxic effect but also enhanced the host antitumor immune response and thus caused high inhibition of tumor growth in experimental glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Yamamoto J, Ogura SI, Shimajiri S, Nakano Y, Akiba D, Kitagawa T, Ueta K, Tanaka T and Nishizawa S: 5-Aminolevulinic acid-induced protoporphyrin IX with multi-dose ionizing irradiation enhances host antitumor response and strongly inhibits tumor growth in experimental glioma in vivo. Mol Med Rep 11: 1813-1819, 2015.
APA
Yamamoto, J., Ogura, S., Shimajiri, S., Nakano, Y., Akiba, D., Kitagawa, T. ... Nishizawa, S. (2015). 5-Aminolevulinic acid-induced protoporphyrin IX with multi-dose ionizing irradiation enhances host antitumor response and strongly inhibits tumor growth in experimental glioma in vivo. Molecular Medicine Reports, 11, 1813-1819. https://doi.org/10.3892/mmr.2014.2991
MLA
Yamamoto, J., Ogura, S., Shimajiri, S., Nakano, Y., Akiba, D., Kitagawa, T., Ueta, K., Tanaka, T., Nishizawa, S."5-Aminolevulinic acid-induced protoporphyrin IX with multi-dose ionizing irradiation enhances host antitumor response and strongly inhibits tumor growth in experimental glioma in vivo". Molecular Medicine Reports 11.3 (2015): 1813-1819.
Chicago
Yamamoto, J., Ogura, S., Shimajiri, S., Nakano, Y., Akiba, D., Kitagawa, T., Ueta, K., Tanaka, T., Nishizawa, S."5-Aminolevulinic acid-induced protoporphyrin IX with multi-dose ionizing irradiation enhances host antitumor response and strongly inhibits tumor growth in experimental glioma in vivo". Molecular Medicine Reports 11, no. 3 (2015): 1813-1819. https://doi.org/10.3892/mmr.2014.2991
Copy and paste a formatted citation
x
Spandidos Publications style
Yamamoto J, Ogura SI, Shimajiri S, Nakano Y, Akiba D, Kitagawa T, Ueta K, Tanaka T and Nishizawa S: 5-Aminolevulinic acid-induced protoporphyrin IX with multi-dose ionizing irradiation enhances host antitumor response and strongly inhibits tumor growth in experimental glioma in vivo. Mol Med Rep 11: 1813-1819, 2015.
APA
Yamamoto, J., Ogura, S., Shimajiri, S., Nakano, Y., Akiba, D., Kitagawa, T. ... Nishizawa, S. (2015). 5-Aminolevulinic acid-induced protoporphyrin IX with multi-dose ionizing irradiation enhances host antitumor response and strongly inhibits tumor growth in experimental glioma in vivo. Molecular Medicine Reports, 11, 1813-1819. https://doi.org/10.3892/mmr.2014.2991
MLA
Yamamoto, J., Ogura, S., Shimajiri, S., Nakano, Y., Akiba, D., Kitagawa, T., Ueta, K., Tanaka, T., Nishizawa, S."5-Aminolevulinic acid-induced protoporphyrin IX with multi-dose ionizing irradiation enhances host antitumor response and strongly inhibits tumor growth in experimental glioma in vivo". Molecular Medicine Reports 11.3 (2015): 1813-1819.
Chicago
Yamamoto, J., Ogura, S., Shimajiri, S., Nakano, Y., Akiba, D., Kitagawa, T., Ueta, K., Tanaka, T., Nishizawa, S."5-Aminolevulinic acid-induced protoporphyrin IX with multi-dose ionizing irradiation enhances host antitumor response and strongly inhibits tumor growth in experimental glioma in vivo". Molecular Medicine Reports 11, no. 3 (2015): 1813-1819. https://doi.org/10.3892/mmr.2014.2991
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