Effect of fibroblast growth factor 9 on the osteogenic differentiation of bone marrow stromal stem cells and dental pulp stem cells

Lu,Jingting; Dai,Jiewen; Wang,Xudong; Zhang,Maolin; Zhang,Peng; Sun,Hao; Zhang,Xiuli; Yu,Hongbo; Zhang,Wenbin; Zhang,Lei; Jiang,Xinquan; Shen,Steve  Guofang

doi:10.3892/mmr.2014.2998

March-2015 Volume 11 Issue 3

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March-2015 Volume 11 Issue 3

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Article Open Access

Effect of fibroblast growth factor 9 on the osteogenic differentiation of bone marrow stromal stem cells and dental pulp stem cells

Authors:
- Jingting Lu
- Jiewen Dai
- Xudong Wang
- Maolin Zhang
- Peng Zhang
- Hao Sun
- Xiuli Zhang
- Hongbo Yu
- Wenbin Zhang
- Lei Zhang
- Xinquan Jiang
- Steve Guofang Shen
View Affiliations / Copyright

Affiliations: Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China, Oral Bioengineering Lab, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China

Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 1661-1668
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Published online on: November 26, 2014

https://doi.org/10.3892/mmr.2014.2998
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Abstract

The role of fibroblast growth factor 9 (FGF9) in bone formation may depend on gene dosage, developmental stage, cell type or interactions with other cytokines. In the present study bone marrow stromal stem cells (BMSCs) and dental pulp stem cells (DPSCs) were cultured and osteogenically induced in vitro, treated with exogenous FGF9 at varying concentrations. Alkaline phosphatase staining, alizarin red S staining, reverse transcription quantitative polymerase chain reaction and western blot analyses were performed in order to investigate the gene expression levels of osteogenic markers. The results of the present study demonstrated that FGF9 enhanced the phosphorylation of extracellular signal‑regulated kinase 1/2 (ERK1/2) during osteogenic induction in BMSCs and DPSCs, which are derived from different tissues. FGF9 also inhibited the osteogenic differentiation of BMSCs and DPSCs through the activation of ERK1/2. These findings suggested that FGF9 may be an inhibitor of osteogenesis in mesenchymal stem cells in vitro and its application in vivo requires investigation in the future.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of fibroblast growth factor 9 on the osteogenic differentiation of bone marrow stromal stem cells and dental pulp stem cells

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