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Article Open Access

Cold ischemia-induced autophagy in rat lung tissue

  • Authors:
    • Xu Chen
    • Jing‑Xiang Wu
    • Xing‑Ji You
    • Hong‑Wei Zhu
    • Jiong‑Lin Wei
    • Mei‑Ying Xu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, P.R. China, Department of Physiology, Second Military Medical University, Shanghai 200433, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2513-2519
    |
    Published online on: November 26, 2014
       https://doi.org/10.3892/mmr.2014.2999
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Abstract

Autophagy is a highly conserved pathway that permits recycling of nutrients within the cell and is rapidly upregulated during starvation or cell stress. Autophagy has been implicated in the pathophysiological process of warm ischemia‑reperfusion injury in the rat lung. Cold ischemia (CI) preservation for lung transplantation also exhibits cell stress and nutrient deprivation, however, little is known with regard to the involvement of autophagy in this process. In the present study, CI preservation‑induced autophagy and apoptosis was investigated in the lungs of Sprague Dawley rats. Sprague Dawley rat lungs were flushed and preserved at 4˚C (i.e. CI) for various durations (0, 3, 6, 12 and 24 h). The levels of autophagy, autophagic cell death and apoptosis were measured at each time point following CI. The results revealed that autophagy was induced by CI preservation, which was initiated at 3 h, peaked at 6 h after CI and declined thereafter. Additionally, a coexistence of autophagic cell death and apoptosis was observed in rat lung tissues following prolonged CI. These findings demonstrate that autophagy is involved in the pathophysiological process of lung CI. Furthermore, autophagic cell death in addition to necrosis and apoptosis occurs following CI in the lung. CI preservation may therefore be a potential mechanism of lung injury during organ preservation prior to lung transplantation.
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1 

de Perrot M, Liu M, Waddell TK and Keshavjee S: Ischemia-reperfusion-induced lung injury. Am J Respir Crit Care Med. 167:490–511. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Novick RJ, Menkis AH and McKenzie FN: New trends in lung preservation: a collective review. J Heart Lung Transplant. 11:377–392. 1992.PubMed/NCBI

3 

de Perrot M and Keshavjee S: Lung preservation. Semin Thorac Cardiovasc Surg. 16:300–308. 2004. View Article : Google Scholar

4 

Thabut G, Mal H, Cerrina J, et al: Graft ischemic time and outcome of lung transplantation: a multicenter analysis. Am J Respir Crit Care Med. 171:786–791. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Lee J, Giordano S and Zhang J: Autophagy, mitochondria and oxidative stress: cross-talk and redox signalling. Biochem J. 441:523–540. 2012. View Article : Google Scholar :

6 

Mizushima N and Komatsu M: Autophagy: renovation of cells and tissues. Cell. 147:728–741. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Ogier-Denis E and Codogno P: Autophagy: a barrier or an adaptive response to cancer. Biochim Biophys Acta. 1603:113–128. 2003.PubMed/NCBI

8 

Shintani T and Klionsky DJ: Autophagy in health and disease: a double-edged sword. Science. 306:990–995. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Bursch W, Karwan A, Mayer M, et al: Cell death and autophagy: cytokines, drugs and nutritional factors. Toxicology. 254:147–157. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Clarke PG: Developmental cell death: morphological diversity and multiple mechanisms. Anat Embryol (Berl). 181:195–213. 1990. View Article : Google Scholar

11 

Suzuki C, Isaka Y, Takabatake Y, et al: Participation of autophagy in renal ischemia/reperfusion injury. Biochem Biophys Res Commun. 368:100–106. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Matsui Y, Takagi H, Qu X, et al: Distinct roles of autophagy in the heart during ischemia and reperfusion: roles of AMP-activated protein kinase and Beclin 1 in mediating autophagy. Circ Res. 100:914–922. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Kanno H, Ozawa H, Sekiguchi A, Yamaya S and Itoi E: Induction of autophagy and autophagic cell death in damaged neural tissue after acute spinal cord injury in mice. Spine (Phila Pa. 1976.36:E1427–E1434. 2011. View Article : Google Scholar

14 

Rami A, Langhagen A and Steiger S: Focal cerebral ischemia induces upregulation of Beclin 1 and autophagy-like cell death. Neurobiol Dis. 29:132–141. 2008. View Article : Google Scholar

15 

Zhang J, Wang JS, Zheng ZK, et al: Participation of autophagy in lung ischemia-reperfusion injury in vivo. J Surg Res. 182:e79–e87. 2012. View Article : Google Scholar

16 

Wu J, Wei J, You X, et al: Inhibition of hydrogen sulfide generation contributes to lung injury after experimental orthotopic lung transplantation. J Surg Res. 182:e25–e33. 2013. View Article : Google Scholar

17 

Kabeya Y, Mizushima N, Ueno T, et al: LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 19:5720–5728. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Ray SK, Matzelle DC, Wilford GG, Hogan EL and Banik NL: E-64-d prevents both calpain upregulation and apoptosis in the lesion and penumbra following spinal cord injury in rats. Brain Res. 867:80–89. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Chen Y, Azad MB and Gibson SB: Methods for detecting autophagy and determining autophagy-induced cell death. Can J Physiol Pharmacol. 88:285–295. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Yang Z and Klionsky DJ: Mammalian autophagy: core molecular machinery and signaling regulation. Curr Opin Cell Biol. 22:124–131. 2010. View Article : Google Scholar :

21 

Mizushima N, Yoshimori T and Levine B: Methods in mammalian autophagy research. Cell. 140:313–326. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Yang Z and Klionsky DJ: Eaten alive: a history of macroautophagy. Nat Cell Biol. 12:814–822. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Okada Y and Kondo T: Preservation solution for lung transplantation. Gen Thorac Cardiovasc Surg. 57:635–639. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Zaouali MA, Ben Abdennebi H, Padrissa-Altés S, Mahfoudh-Boussaid A and Roselló-Catafau J: Pharmacological strategies against cold ischemia reperfusion injury. Expert Opin Pharmacother. 11:537–555. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Lu Z, Dono K, Gotoh K, et al: Participation of autophagy in the degeneration process of rat hepatocytes after transplantation following prolonged cold preservation. Arch Histol Cytol. 68:71–80. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Gotoh K, Lu Z, Morita M, et al: Participation of autophagy in the initiation of graft dysfunction after rat liver transplantation. Autophagy. 5:351–360. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Minor T, Stegemann J, Hirner A and Koetting M: Impaired autophagic clearance after cold preservation of fatty livers correlates with tissue necrosis upon reperfusion and is reversed by hypothermic reconditioning. Liver Transpl. 15:798–805. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Padrissa-Altés S, Zaouali MA, Bartrons R and Roselló-Catafau J: Ubiquitin-proteasome system inhibitors and AMPK regulation in hepatic cold ischaemia and reperfusion injury: possible mechanisms. Clin Sci (Lond). 123:93–98. 2012. View Article : Google Scholar

29 

Turkmen K, Martin J, Akcay A, et al: Apoptosis and autophagy in cold preservation ischemia. Transplantation. 91:1192–1197. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Jiang M, Liu K, Luo J and Dong Z: Autophagy is a renoprotective mechanism during in vitro hypoxia and in vivo ischemia-reperfusion injury. Am J Pathol. 176:1181–1192. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Kaushal GP: Autophagy protects proximal tubular cells from injury and apoptosis. Kidney Int. 82:1250–1253. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Petrovski G, Das S, Juhasz B, Kertesz A, Tosaki A and Das DK: Cardioprotection by endoplasmic reticulum stress-induced autophagy. Antioxid Redox Signal. 14:2191–2200. 2011. View Article : Google Scholar

33 

Jiang S, Guo R, Zhang Y, Zou Y and Ren J: Heavy metal scavenger metallothionein mitigates deep hypothermia-induced myocardial contractile anomalies: role of autophagy. Am J Physiol Endocrinol Metab. 304:E74–E86. 2013. View Article : Google Scholar :

34 

Hamacher-Brady A, Brady NR and Gottlieb RA: Enhancing macroautophagy protects against ischemia/reperfusion injury in cardiac myocytes. J Biol Chem. 281:29776–29787. 2006. View Article : Google Scholar : PubMed/NCBI

35 

Loos B, Genade S, Ellis B, Lochner A and Engelbrecht AM: At the core of survival: autophagy delays the onset of both apoptotic and necrotic cell death in a model of ischemic cell injury. Exp Cell Res. 317:1437–1453. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Gurusamy N, Lekli I, Gorbunov NV, Gherghiceanu M, Popescu LM and Das DK: Cardioprotection by adaptation to ischaemia augments autophagy in association with BAG-1 protein. J Cell Mol Med. 13:373–387. 2009. View Article : Google Scholar

37 

Huang C, Yitzhaki S, Perry CN, et al: Autophagy induced by ischemic preconditioning is essential for cardioprotection. J Cardiovasc Transl Res. 3:365–373. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Sala-Mercado JA, Wider J, Undyala VV, et al: Profound cardioprotection with chloramphenicol succinate in the swine model of myocardial ischemia-reperfusion injury. Circulation. 122:S179–S184. 2010. View Article : Google Scholar : PubMed/NCBI

39 

Shi R, Weng J, Zhao L, Li XM, Gao TM and Kong J: Excessive autophagy contributes to neuron death in cerebral ischemia. CNS Neurosci Ther. 18:250–260. 2012. View Article : Google Scholar : PubMed/NCBI

40 

Balduini W, Carloni S and Buonocore G: Autophagy in hypoxia-ischemia induced brain injury. J Matern Fetal Neonatal Med. 25 Suppl 1:30–34. 2012. View Article : Google Scholar : PubMed/NCBI

41 

Fischer S, Cassivi SD, Xavier AM, et al: Cell death in human lung transplantation: apoptosis induction in human lungs during ischemia and after transplantation. Ann Surg. 231:424–431. 2000. View Article : Google Scholar : PubMed/NCBI

42 

Ng CS, Wan S and Yim AP: Pulmonary ischaemia-reperfusion injury: role of apoptosis. Eur Respir J. 25:356–363. 2005. View Article : Google Scholar : PubMed/NCBI

43 

Kitanaka C and Kuchino Y: Caspase-independent programmed cell death with necrotic morphology. Cell Death Differ. 6:508–515. 1999. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Wu JX, You XJ, Zhu HW, Wei JL and Xu MY: Cold ischemia-induced autophagy in rat lung tissue. Mol Med Rep 11: 2513-2519, 2015.
APA
Chen, X., Wu, J., You, X., Zhu, H., Wei, J., & Xu, M. (2015). Cold ischemia-induced autophagy in rat lung tissue. Molecular Medicine Reports, 11, 2513-2519. https://doi.org/10.3892/mmr.2014.2999
MLA
Chen, X., Wu, J., You, X., Zhu, H., Wei, J., Xu, M."Cold ischemia-induced autophagy in rat lung tissue". Molecular Medicine Reports 11.4 (2015): 2513-2519.
Chicago
Chen, X., Wu, J., You, X., Zhu, H., Wei, J., Xu, M."Cold ischemia-induced autophagy in rat lung tissue". Molecular Medicine Reports 11, no. 4 (2015): 2513-2519. https://doi.org/10.3892/mmr.2014.2999
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Wu JX, You XJ, Zhu HW, Wei JL and Xu MY: Cold ischemia-induced autophagy in rat lung tissue. Mol Med Rep 11: 2513-2519, 2015.
APA
Chen, X., Wu, J., You, X., Zhu, H., Wei, J., & Xu, M. (2015). Cold ischemia-induced autophagy in rat lung tissue. Molecular Medicine Reports, 11, 2513-2519. https://doi.org/10.3892/mmr.2014.2999
MLA
Chen, X., Wu, J., You, X., Zhu, H., Wei, J., Xu, M."Cold ischemia-induced autophagy in rat lung tissue". Molecular Medicine Reports 11.4 (2015): 2513-2519.
Chicago
Chen, X., Wu, J., You, X., Zhu, H., Wei, J., Xu, M."Cold ischemia-induced autophagy in rat lung tissue". Molecular Medicine Reports 11, no. 4 (2015): 2513-2519. https://doi.org/10.3892/mmr.2014.2999
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