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Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model

  • Authors:
    • Wei Wang
    • Xiayu Li
    • Danwei Zheng
    • Decai Zhang
    • Xiaoqing  Peng
    • Xuemei Zhang
    • Feiyan Ai
    • Xiaoyan Wang
    • Jian Ma
    • Wei Xiong
    • Guiyuan Li
    • Yanhong Zhou
    • Shourong Shen
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Key Laboratory of Carcinogenesis, Ministry of Education, Cancer Research Institute, Changsha, Hunan 410078, P.R. China, Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, Changsha, Hunan 410078, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2397-2406
    |
    Published online on: November 28, 2014
       https://doi.org/10.3892/mmr.2014.3018
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Abstract

The high risk of developing colorectal carcinoma (CRC), from ulcerative colitis (UC), is well known. Macrophages are widely distributed immune cells that have an indispensable role in UC, as well as in CRC. However, little is currently known about the dynamic changes that occur in macrophage and M1/M2 macrophage subpopulations, during UC‑associated carcinogenesis. The aim of the present study was to investigate the alteration of colorectal macrophages and M1/M2 macrophage subpopulations during UC‑associated carcinogenesis. Both expression level alterations and functional changes were determined during UC‑associated carcinogenesis in an azoxymethane/dextran sodium sulfate‑induced chemically colitis‑associated carcinoma mouse model of Crj:CD‑1 (ICR) mice. Notable evidence from immunohistochemistry, flow cytometry, cytokine detection, and gene expression analyses demonstrated that M2 macrophages have a critical role in CRC initiation, promotion, and metastasis. M2 macrophages are associated with unbalanced pro‑inflammatory and anti‑inflammatory axes and aberrant enhancement of migration/invasion‑associated factors. Functional changes, similar to M2 polarized macrophages, were shown to occur in the M1 macrophages, without phenotypical changes, during the development of carcinoma and metastasis. The results of the present study suggest that M2 macrophages have a pro‑tumor role during UC‑associated carcinogenesis. Furthermore, similar functional changes occurred in the M1 macrophages, without polarization alterations, during carcinogenesis and metastasis.
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View References

1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global Cancer Statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Nathan C and Ding A: Nonresolving inflammation. Cell. 140:871–882. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Lakatos L, Mester G, Erdelyi Z, et al: Risk factors for ulcerative colitis-associated colorectal cancer in a Hungarian cohort of patients with ulcerative colitis: results of a population-based study. Inflamm Bowel Dis. 12:205–211. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Lakatos PL and Lakatos L: Risk for colorectal cancer in ulcerative colitis: changes, causes and management strategies. World J Gastroenterol. 14:3937–3947. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Gong W, Lv N, Wang B, et al: Risk of ulcerative colitis-associated colorectal cancer in China: a multi-center retrospective study. Dig Dis Sci. 57:503–507. 2012. View Article : Google Scholar

6 

Zisman TL and Rubin DT: Colorectal cancer and dysplasia in inflammatory bowel disease. World J Gastroenterol. 14:2662–2669. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Karin M: Nuclear factor-kappaB in cancer development and progression. Nature. 441:431–436. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Pikarsky E, Porat RM, Stein I, et al: NF-kappaB functions as a tumour promoter in inflammation-associated cancer. Nature. 431:461–466. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Bollrath J, Phesse TJ, von Burstin VA, et al: gp130-mediated Stat3 activation in enterocytes regulates cell survival and cell-cycle progression during colitis-associated tumorigenesis. Cancer Cell. 15:91–102. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Grivennikov S, Karin E, Terzic J, et al: IL-6 and Stat3 are required for survival of intestinal epithelial cells and development of colitis-associated cancer. Cancer Cell. 15:103–113. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Baumgart DC and Sandborn WJ: Inflammatory bowel disease: clinical aspects and established and evolving therapies. Lancet. 369:1641–1657. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Mosser DM and Edwards JP: Exploring the full spectrum of macrophage activation. Nat Rev Immunol. 8:958–969. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Lawrence T and Natoli G: Transcriptional regulation of macrophage polarization: enabling diversity with identity. Nat Rev Immunol. 11:750–761. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Martinez FO, Sica A, Mantovani A and Locati M: Macrophage activation and polarization. Front Biosci. 13:453–461. 2008. View Article : Google Scholar

15 

Siveen KS and Kuttan G: Role of macrophages in tumour progression. Immunol Lett. 123:97–102. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Xie J and Itzkowitz SH: Cancer in inflammatory bowel disease. World J Gastroenterol. 14:378–389. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Bar-On L, Zigmond E and Jung S: Management of gut inflammation through the manipulation of intestinal dendritic cells and macrophages? Semin Immunol. 23:58–64. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Sica A, Schioppa T, Mantovani A and Allavena P: Tumor-associated macrophages are a distinct M2 polarised population promoting tumor progression: potential targets of anti-cancer therapy. Eur J Cancer. 42:717–727. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Bailey C, Negus R, Morris A, et al: Chemokine expression is associated with the accumulation of tumour associated macrophages (TAMs) and progression in human colorectal cancer. Clin Exp Metastasis. 24:121–130. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Sica A, Allavena P and Mantovani A: Cancer related inflammation: the macrophage connection. Cancer Lett. 267:204–215. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Green CE, Liu T, Montel V, et al: Chemoattractant signaling between tumor cells and macrophages regulated cancer cell migration, metastasis and neovascularization. PLoS One. 4:e67132009. View Article : Google Scholar

22 

Wirtz S, Neufert C, Weigmann B and Neurath MF: Chemically induced mouse models of intestinal inflammation. Nat Protoc. 2:541–546. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Clapper ML, Cooper HS and Chang WC: Dextran sulfate sodium-induced colitis-associated neoplasia: a promising model for the development of chemopreventive interventions. Acta Pharmacol Sin. 28:1450–1459. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Lai CS, Tsai ML, Cheng AC, et al: Chemoprevention of colonic tumorigenesis by dietary hydroxylated polymethoxyflavones in azoxymethane-treated mice. Mol Nutr Food Res. 55:278–290. 2011. View Article : Google Scholar

25 

Cooper HS, Murthy SN, Shah RS and Sedergran DJ: Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest. 69:238–249. 1993.PubMed/NCBI

26 

Forssell J, Oberg A, Henriksson ML, Stenling R, Jung A and Palmqvist R: High macrophage infiltration along the tumor front correlates with improved survival in colon cancer. Clin Cancer Res. 13:1472–1479. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Mantovani A, Sica A and Locati M: Macrophage polarization comes of age. Immunity. 23:344–346. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Mantovani A: Inflammation and cancer: the macrophage connection. Medicina. 67:32–34. 2007.

29 

Mantovani A: Tumor-associated macrophages in cancer-related inflammation. Immunotherapy. 3:21–22. 2011. View Article : Google Scholar

30 

Ong SM, Tan YC, Beretta O, et al: Macrophages in human colorectal cancer are pro-inflammatory and prime T cells towards an anti-tumour type-1 inflammatory response. Eur J Immunol. 42:89–100. 2012. View Article : Google Scholar

31 

Condeelis J and Pollard JW: Macrophages: obligate partners for tumor cell migration, invasion, and metastasis. Cell. 124:263–266. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Zhou Q, Peng RQ, Wu XJ, et al: The density of macrophages in the invasive front is inversely correlated to liver metastasis in colon cancer. J Transl Med. 8:132010. View Article : Google Scholar : PubMed/NCBI

33 

Solinas G, Germano G, Mantovani A and Allavena P: Tumor-associated macrophages (TAM) as major players of the cancer-related inflammation. J Leukoc Biol. 86:1065–1073. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Imtiyaz HZ, Williams EP, Hickey MM, et al: Hypoxia-inducible factor 2alpha regulates macrophage function in mouse models of acute and tumor inflammation. J Clin Invest. 120:2699–2714. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Low-Marchelli JM, Ardi VC, Vizcarra EA, van Rooijen N, Quigley JP and Yang J: Twist1 induces CCL2 and recruits macrophages to promote angiogenesis. Cancer Res. 73:662–671. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang W, Li X, Zheng D, Zhang D, Peng X, Zhang X, Ai F, Wang X, Ma J, Xiong W, Xiong W, et al: Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model. Mol Med Rep 11: 2397-2406, 2015.
APA
Wang, W., Li, X., Zheng, D., Zhang, D., Peng, X., Zhang, X. ... Shen, S. (2015). Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model. Molecular Medicine Reports, 11, 2397-2406. https://doi.org/10.3892/mmr.2014.3018
MLA
Wang, W., Li, X., Zheng, D., Zhang, D., Peng, X., Zhang, X., Ai, F., Wang, X., Ma, J., Xiong, W., Li, G., Zhou, Y., Shen, S."Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model". Molecular Medicine Reports 11.4 (2015): 2397-2406.
Chicago
Wang, W., Li, X., Zheng, D., Zhang, D., Peng, X., Zhang, X., Ai, F., Wang, X., Ma, J., Xiong, W., Li, G., Zhou, Y., Shen, S."Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model". Molecular Medicine Reports 11, no. 4 (2015): 2397-2406. https://doi.org/10.3892/mmr.2014.3018
Copy and paste a formatted citation
x
Spandidos Publications style
Wang W, Li X, Zheng D, Zhang D, Peng X, Zhang X, Ai F, Wang X, Ma J, Xiong W, Xiong W, et al: Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model. Mol Med Rep 11: 2397-2406, 2015.
APA
Wang, W., Li, X., Zheng, D., Zhang, D., Peng, X., Zhang, X. ... Shen, S. (2015). Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model. Molecular Medicine Reports, 11, 2397-2406. https://doi.org/10.3892/mmr.2014.3018
MLA
Wang, W., Li, X., Zheng, D., Zhang, D., Peng, X., Zhang, X., Ai, F., Wang, X., Ma, J., Xiong, W., Li, G., Zhou, Y., Shen, S."Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model". Molecular Medicine Reports 11.4 (2015): 2397-2406.
Chicago
Wang, W., Li, X., Zheng, D., Zhang, D., Peng, X., Zhang, X., Ai, F., Wang, X., Ma, J., Xiong, W., Li, G., Zhou, Y., Shen, S."Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model". Molecular Medicine Reports 11, no. 4 (2015): 2397-2406. https://doi.org/10.3892/mmr.2014.3018
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