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Article

Comparative analysis of cytotoxic T lymphocyte response induced by dendritic cells pulsed with recombinant adeno-associated virus carrying α-fetoprotein gene or cancer cell lysate

  • Authors:
    • Jun Zhou
    • Ping Ma
    • Jun Li
    • Wei Song
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Opthalmology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China
  • Pages: 3174-3180
    |
    Published online on: December 8, 2014
       https://doi.org/10.3892/mmr.2014.3059
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Abstract

Hepatocellular carcinoma (HCC) is one of the most common and difficult to treat types of cancer worldwide. Antigen‑targeted immunotherapy has the potential to be a novel and effective adjuvant for use in HCC. In the present study, recombinant adeno‑associated virus carrying the α‑fetoprotein gene (rAAV/AFP) and cancer cell lysates were used to pulse antigen‑presenting dendritic cells (DCs) in order to stimulate a cytotoxic T lymphocyte (CTL) response against HCC. rAAV/AFP‑pulsed and cancer cell lysate‑pulsed DCs resulted in a mature DC phenotype with high expression of major histocompatibility complex (MHC) class I, MHC class II, CD80, CD83 and CD86 molecules. However, rAAV/AFP‑pulsed DCs exhibited superiority over cancer cell lysate‑pulsed DCs in terms of stimulating proliferation of T cells, activating T cells to secret interferon‑γ (IFN‑γ) and inducing an AFP‑specific MHC class I‑restricted CTL response. The current data suggest that pulsing of DCs using rAAV/AFP is more effective than the cancer cell lysate‑pulsing technique, and that this technique may be used for the development of immunotherapy in AFP‑positive HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou J, Ma P, Li J and Song W: Comparative analysis of cytotoxic T lymphocyte response induced by dendritic cells pulsed with recombinant adeno-associated virus carrying α-fetoprotein gene or cancer cell lysate. Mol Med Rep 11: 3174-3180, 2015.
APA
Zhou, J., Ma, P., Li, J., & Song, W. (2015). Comparative analysis of cytotoxic T lymphocyte response induced by dendritic cells pulsed with recombinant adeno-associated virus carrying α-fetoprotein gene or cancer cell lysate. Molecular Medicine Reports, 11, 3174-3180. https://doi.org/10.3892/mmr.2014.3059
MLA
Zhou, J., Ma, P., Li, J., Song, W."Comparative analysis of cytotoxic T lymphocyte response induced by dendritic cells pulsed with recombinant adeno-associated virus carrying α-fetoprotein gene or cancer cell lysate". Molecular Medicine Reports 11.4 (2015): 3174-3180.
Chicago
Zhou, J., Ma, P., Li, J., Song, W."Comparative analysis of cytotoxic T lymphocyte response induced by dendritic cells pulsed with recombinant adeno-associated virus carrying α-fetoprotein gene or cancer cell lysate". Molecular Medicine Reports 11, no. 4 (2015): 3174-3180. https://doi.org/10.3892/mmr.2014.3059
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou J, Ma P, Li J and Song W: Comparative analysis of cytotoxic T lymphocyte response induced by dendritic cells pulsed with recombinant adeno-associated virus carrying α-fetoprotein gene or cancer cell lysate. Mol Med Rep 11: 3174-3180, 2015.
APA
Zhou, J., Ma, P., Li, J., & Song, W. (2015). Comparative analysis of cytotoxic T lymphocyte response induced by dendritic cells pulsed with recombinant adeno-associated virus carrying α-fetoprotein gene or cancer cell lysate. Molecular Medicine Reports, 11, 3174-3180. https://doi.org/10.3892/mmr.2014.3059
MLA
Zhou, J., Ma, P., Li, J., Song, W."Comparative analysis of cytotoxic T lymphocyte response induced by dendritic cells pulsed with recombinant adeno-associated virus carrying α-fetoprotein gene or cancer cell lysate". Molecular Medicine Reports 11.4 (2015): 3174-3180.
Chicago
Zhou, J., Ma, P., Li, J., Song, W."Comparative analysis of cytotoxic T lymphocyte response induced by dendritic cells pulsed with recombinant adeno-associated virus carrying α-fetoprotein gene or cancer cell lysate". Molecular Medicine Reports 11, no. 4 (2015): 3174-3180. https://doi.org/10.3892/mmr.2014.3059
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