Upregulation of D site of albumin promoter binding protein in the brain of patients with intractable epilepsy

Yuan,Jinxian; Guo,Jing; Zhang,Melin; Wang,Qian; Huang,Hao; Chen,Yangmei

doi:10.3892/mmr.2014.3065

April-2015 Volume 11 Issue 4

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April-2015 Volume 11 Issue 4

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Upregulation of D site of albumin promoter binding protein in the brain of patients with intractable epilepsy

Authors:
- Jinxian Yuan
- Jing Guo
- Melin Zhang
- Qian Wang
- Hao Huang
- Yangmei Chen
View Affiliations / Copyright

Affiliations: Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China

Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 2486-2492
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Published online on: December 9, 2014

https://doi.org/10.3892/mmr.2014.3065
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Abstract

The mechanisms that underlie the pathogenesis of intractable epilepsy (IE) remain to be elucidated. The present study aimed to investigate the expression of D site of albumin promoter binding protein (DBP) and mitogen‑activated protein kinases (MAPKs) in the temporal lobes of patients with IE, in order to examine the possible roles of DBP in the pathogenesis of IE. The expression of DBP and MAPK was detected by immunohistochemistry and double‑label immunofluorescence staining against DBP/MAPK in 35 patients with IE, and the data were compared with those of the 15 controls. The results demonstrated that DBP expression in IE group (0.31±0.03) was significantly higher compared with that in the controls (0.18±0.02; P<0.05) and MAPK expression in the IE group (0.19±0.03) was also higher compared with that in the controls (0.12±0.02; P<0.05). DBP and MAPK were mainly expressed in the cytoplasm of neurons and the double‑label immunofluorescence staining demonstrated that DBP and MAPK expression occurred in the same neurons. Therefore, the expression of DBP and MAPK in epilepsy patients was upregulated, suggesting a possible pathogenetic role in IE.

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1	Shorvon SD: The epidemiology and treatment of chronic and refractory epilepsy. Epilepsia. 37(Suppl 2): S1–S3. 1996. View Article : Google Scholar : PubMed/NCBI
2	Gachon F, Nagoshi E, Brown SA, Ripperger J and Schibler U: The mammalian circadian timing system: from gene expression to physiology. Chromosoma. 113:103–112. 2004. View Article : Google Scholar : PubMed/NCBI
3	Gachon F, Olela FF, Schaad O, Descombes P and Schibler U: The circadian PAR-domain basic leucine zipper transcription factors DBP, TEF, and HLF modulate basal and inducible xenobiotic detoxification. Cell Metab. 4:25–36. 2006. View Article : Google Scholar : PubMed/NCBI
4	Fonjallaz P, Ossipow V, Wanner G and Schibler U: The two PAR leucine zipper proteins, TEF and DBP, display similar circadian and tissue-specific expression, but have different target promoter preferences. EMBO J. 15:351–362. 1996.PubMed/NCBI
5	Gachon F, Fonjallaz P, Damiola F, et al: The loss of circadian PAR bZip transcription factors results in epilepsy. Genes Dev. 18:1397–1412. 2004. View Article : Google Scholar : PubMed/NCBI
6	Klugmann M, Leichtlein CB, Symes CW, et al: A novel role of circadian transcription factor DBP in hippocampal plasticity. Mol Cell Neurosci. 31:303–314. 2006. View Article : Google Scholar
7	Lopez-Molina L, Conquet F, Dubois-Dauphin M and Schibler U: The DBP gene is expressed according to a circadian rhythm in the suprachiasmatic nucleus and influences circadian behavior. EMBO J. 16:6762–6771. 1997. View Article : Google Scholar
8	Franken P, Lopez-Molina L, Marcacci L, Schibler U and Tafti M: The transcription factor DBP affects circadian sleep consolidation and rhythmic EEG activity. J Neurosci. 20:617–625. 2000.PubMed/NCBI
9	Niculescu AB III, Segal DS, Kuczenski R, Barrett T, Hauger RL and Kelsoe JR: Identifying a series of candidate genes for mania and psychosis: a convergent functional genomics approach. Physiol Genomics. 4:83–91. 2000.PubMed/NCBI
10	Siow YL and Dakshinamurti K: Effect of pyridoxine deficiency on aromatic L-amino acid decarboxylase in adult rat brain. Exp Brain Res. 59:575–581. 1985. View Article : Google Scholar : PubMed/NCBI
11	Sharma SK and Dakshinamurti K: Seizure activity in pyridoxine-deficient adult rats. Epilepsia. 33:235–247. 1992. View Article : Google Scholar : PubMed/NCBI
12	Schlesinger K and Lieff B: Levels of pyridoxine and susceptibility to electroconvulsive and audiogenic seizures. Psychopharmacologia. 42:27–32. 1975. View Article : Google Scholar : PubMed/NCBI
13	During MJ, Cao L, Zuzga DS, et al: Glucagon-like peptide-1 receptor is involved in learning and neuroprotection. Nat Med. 9:1173–1179. 2003. View Article : Google Scholar : PubMed/NCBI
14	Yin Y, Huang P, Han Z, et al: Collagen nanofibers facilitated presynaptic maturation in differentiated neurons from spinal-cord-derived neural stem cells through MAPK/ERK1/2-Synapsin I signaling pathway. Biomacromolecules. 15:2449–2460. 2014.PubMed/NCBI
15	McClelland AC, Hruska M, Coenen AJ, et al: Trans-synaptic EphB2-ephrin-B3 interaction regulates excitatory synapse density by inhibition of postsynaptic MAPK signaling. Proc Natl Acad Sci USA. 107:8830–8835. 2010. View Article : Google Scholar : PubMed/NCBI
16	Mao LM, Reusch JM, Fibuch EE, Liu Z and Wang JQ: Amphetamine increases phosphorylation of MAPK/ERK at synaptic sites in the rat striatum and medial prefrontal cortex. Brain Res. 1494:101–108. 2013. View Article : Google Scholar :

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Upregulation of D site of albumin promoter binding protein in the brain of patients with intractable epilepsy

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