Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling

Liu,Cun‑Fei; Zhang,Jia; Shen,Kai; Gao,Ping‑Jin; Wang,Hai‑Ya; Jin,Xin; Meng,Chao; Fang,Ning‑Yuan

doi:10.3892/mmr.2014.3069

Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling

Authors:
- Cun‑Fei Liu
- Jia Zhang
- Kai Shen
- Ping‑Jin Gao
- Hai‑Ya Wang
- Xin Jin
- Chao Meng
- Ning‑Yuan Fang
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Affiliations: Department of Geriatrics, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, P.R. China, Division of Cardiology, Zhoushan People's Hospital, Zhoushan, Zhejiang 316000, P.R. China, Shanghai Key Laboratory of Vascular Biology at Ruijin Hospital and Shanghai Institute of Hypertension, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China
Published online on: December 10, 2014 https://doi.org/10.3892/mmr.2014.3069
Pages: 2608-2614
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Vascular adventitia and adventitia‑derived reactive oxygen species (ROS) contribute to vascular remodeling following vascular injury. A previous ex vivo study in adventitial fibroblasts showed that catalase, one of most important anti‑oxide enzymes, was downregulated by angiotensin II (AngII). The aim of the present study was to investigate whether adventitial gene transfer of catalase affects AngII‑induced vascular remodeling in vivo. Adenoviruses co‑expressing catalase and enhanced green fluorescent protein (eGFP) or expressing eGFP only were applied to the adventitial surface of common carotid arteries of Sprague‑Dawley rats. Alzet minipumps administering AngII (0.75 mg/kg/day) were then implanted subcutaneously for 14 days. Systolic blood pressure and biological parameters of vascular remodeling were measured in each group. Adventitial fibroblasts were cultured and p38 mitogen‑activated protein kinase (MAPK) phosphorylation was measured using western blot analysis. The results showed that adventitial gene transfer of catalase had no effect on AngII‑induced systolic blood pressure elevation. However, catalase adenovirus transfection significantly inhibited AngII‑induced media hypertrophy compared with that of the control virus (P<0.05). In addition, catalase transfection significantly attenuated AngII‑induced ROS generation, macrophage infiltration, collagen deposition and adventitial α‑smooth muscle actin expression. Furthermore, catalase transfection significantly inhibited the AngII‑induced increase in p38MAPK phosphorylation. In conclusion, the results of the present study demonstrated that adventitial gene transfer of catalase significantly attenuated AngII‑induced vascular remodeling in rats via inhibition of adventitial p38MAPK phosphorylation.

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