Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
April-2015 Volume 11 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2015 Volume 11 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling

  • Authors:
    • Cun‑Fei Liu
    • Jia Zhang
    • Kai Shen
    • Ping‑Jin Gao
    • Hai‑Ya Wang
    • Xin Jin
    • Chao Meng
    • Ning‑Yuan Fang
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, P.R. China, Division of Cardiology, Zhoushan People's Hospital, Zhoushan, Zhejiang 316000, P.R. China, Shanghai Key Laboratory of Vascular Biology at Ruijin Hospital and Shanghai Institute of Hypertension, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2608-2614
    |
    Published online on: December 10, 2014
       https://doi.org/10.3892/mmr.2014.3069
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Vascular adventitia and adventitia‑derived reactive oxygen species (ROS) contribute to vascular remodeling following vascular injury. A previous ex vivo study in adventitial fibroblasts showed that catalase, one of most important anti‑oxide enzymes, was downregulated by angiotensin II (AngII). The aim of the present study was to investigate whether adventitial gene transfer of catalase affects AngII‑induced vascular remodeling in vivo. Adenoviruses co‑expressing catalase and enhanced green fluorescent protein (eGFP) or expressing eGFP only were applied to the adventitial surface of common carotid arteries of Sprague‑Dawley rats. Alzet minipumps administering AngII (0.75 mg/kg/day) were then implanted subcutaneously for 14 days. Systolic blood pressure and biological parameters of vascular remodeling were measured in each group. Adventitial fibroblasts were cultured and p38 mitogen‑activated protein kinase (MAPK) phosphorylation was measured using western blot analysis. The results showed that adventitial gene transfer of catalase had no effect on AngII‑induced systolic blood pressure elevation. However, catalase adenovirus transfection significantly inhibited AngII‑induced media hypertrophy compared with that of the control virus (P<0.05). In addition, catalase transfection significantly attenuated AngII‑induced ROS generation, macrophage infiltration, collagen deposition and adventitial α‑smooth muscle actin expression. Furthermore, catalase transfection significantly inhibited the AngII‑induced increase in p38MAPK phosphorylation. In conclusion, the results of the present study demonstrated that adventitial gene transfer of catalase significantly attenuated AngII‑induced vascular remodeling in rats via inhibition of adventitial p38MAPK phosphorylation.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Raaz U, Toh R, Maegdefessel L, et al: Hemodynamic regulation of reactive oxygen species: implications for vascular diseases. Antioxid Redox Signal. 20:914–928. 2014. View Article : Google Scholar :

2 

Juni RP, Duckers HJ, Vanhoutte PM, Virmani R and Moens AL: Oxidative stress and pathological changes after coronary artery interventions. J Am Coll Cardiol. 61:1471–1481. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Paravicini TM and Touyz RM: Redox signaling in hypertension. Cardiovasc Res. 71:247–258. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Clempus RE and Griendling KK: Reactive oxygen species signaling in vascular smooth muscle cells. Cardiovas Res. 71:216–225. 2006. View Article : Google Scholar

5 

Schulz E, Gori T and Münzel T: Oxidative stress and endothelial dysfunction in hypertension. Hypertens Res. 34:665–673. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Stenmark KR, Yeager ME, El Kasmi KC, et al: The adventitia: essential regulator of vascular wall structure and function. Annu Rev Physiol. 75:23–47. 2013. View Article : Google Scholar :

7 

Sartore S, Chiavegato A, Faggin E, et al: Contribution of adventitial fibroblasts to neointima formation and vascular remodeling: from innocent bystander to active participant. Circ Res. 89:1111–1121. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Liu J, Ormsby A, Oja-Tebbe N and Pagano PJ: Gene transfer of NAD (P) H oxidase inhibitor to the vascular adventitia attenuates medial smooth muscle hypertrophy. Circ Res. 95:587–594. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Tieu BC, Ju X, Lee C, et al: Aortic adventitial fibroblasts participate in angiotensin-induced vascular wall inflammation and remodeling. J Vasc Res. 48:261–272. 2011. View Article : Google Scholar

10 

Pagano PJ, Chanock SJ, Siwik DA, Colucci WS and Clark JK: Angiotensin II induces p67phox mRNA expression and NADPH oxidase superoxide generation in rabbit aortic adventitial fibroblasts. Hypertension. 32:331–337. 1998. View Article : Google Scholar : PubMed/NCBI

11 

Yang W, Zhang J, Wang H, et al: Angiotensin II downregulates catalase expression and activity in vascular adventitial fibroblasts through an AT1R/ERK1/2-dependent pathway. Mol Cell Biochem. 358:21–29. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Schneider DB, Sassani AB, Vassalli G, Driscoll RM and Dichek DA: Adventitial delivery minimizes the proinflammatory effects of adenoviral vectors. J Vasc Surg. 29:543–550. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Laitinen M and Yla-Herttuala S: Adventitial gene transfer to arterial wall. Pharmacol Res. 37:251–254. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Dourron HM, Jacobson GM, Park JL, et al: Perivascular gene transfer of NADPH oxidase inhibitor suppresses angioplasty-induced neointimal proliferation of rat carotid artery. Am J Physiol Heart Circ Physiol. 288:H946–H953. 2005. View Article : Google Scholar

15 

Ozumi K, Tasaki H, Takatsu H, et al: Extracellular superoxide dismutase overexpression reduces cuff-induced arterial neointimal formation. Atherosclerosis. 181:55–62. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Tollefson AE, Kuppuswamy M, Shashkova EV, Doronin K and Wold WS: Preparation and titration of CsCl-banded adenovirus stocks. Methods Mol Med. 130:223–235. 2007.PubMed/NCBI

17 

Ducharme A, Frantz S, Aikawa M, et al: Targeted deletion of matrix metalloproteinase-9 attenuates left ventricular enlargement and collagen accumulation after experimental myocardial infarction. J Clin Invest. 106:55–62. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Siow RC, Mallawaarachchi CM and Weissberg PL: Migration of adventitial myofibroblasts following vascular balloon injury: insights from in vivo gene transfer to rat carotid arteries. Cardiovasc Res. 59:212–221. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Chan SH, Hsu KS, Huang CC, Wang LL, Ou CC and Chan JY: NADPH oxidase-derived superoxide anion mediates angiotensin II-induced pressor effect via activation of p38 mitogen-activated protein kinase in the rostral ventrolateral medulla. Circ Res. 97:772–780. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Ushio-Fukai M, Alexander RW, Akers M and Griendling KK: P38 mitogen-activated protein kinase is a critical component of the redox-sensitive signaling pathways activated by angiotensin II. Role in vascular smooth muscle cell hypertrophy. J Biol Chem. 273:15022–15029. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Di Wang H, Rätsep MT, Chapman A and Boyd R: Adventitial fibroblasts in vascular structure and function: the role of oxidative stress and beyond. Can J Physiol and Pharmacol. 88:177–186. 2010. View Article : Google Scholar

22 

Havelka GE and Kibbe MR: The vascular adventitia: its role in the arterial injury response. Vasc Endovascular Surg. 45:381–390. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Shi Y, O’Brien JE, Fard A, Mannion JD, Wang D and Zalewski A: Adventitial myofibroblasts contribute to neointimal formation in injured porcine coronary arteries. Circulation. 94:1655–1664. 1996. View Article : Google Scholar : PubMed/NCBI

24 

Haurani MJ and Pagano PJ: Adventitial fibroblast reactive oxygen species as autacrine and paracrine mediators of remodeling: bellwether for vascular disease? Cardiovasc Res. 75:679–689. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Di Wang H, Hope S, Du Y, et al: Paracrine role of adventitial superoxide anion in mediating spontaneous tone of the isolated rat aorta in angiotensin II-induced hypertension. Hypertension. 33:1225–1232. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Bishop JE and Lindahl G: Regulation of cardiovascular collagen synthesis by mechanical load. Cardiovasc Res. 42:27–44. 1999. View Article : Google Scholar : PubMed/NCBI

27 

Al Ghouleh I, Khoo NK, Knaus UG, et al: Oxidases and peroxidases in cardiovascular and lung disease: new concepts in reactive oxygen species signaling. Free Radic Biol Med. 51:1271–1288. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Weaver M, Liu J, Pimentel D, et al: Adventitial delivery of dominant-negative p67phox attenuates neointimal hyperplasia of the rat carotid artery. Am J Physiol Heart Circ Physiol. 290:H1933–H1941. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Mallawaarachchi CM, Weissberg PL and Siow RC: Smad7 gene transfer attenuates adventitial cell migration and vascular remodeling after balloon injury. Arterioscler Thromb Vasc Biol. 25:1383–1387. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Siow RC and Churchman AT: Adventitial growth factor signalling and vascular remodelling: potential of perivascular gene transfer from the outside-in. Cardiovasc Res. 75:659–668. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Maiellaro K and Taylor WR: The role of the adventitia in vascular inflammation. Cardiovasc Res. 75:640–648. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Okamoto E, Couse T, De Leon H, et al: Perivascular inflammation after balloon angioplasty of porcine coronary arteries. Circulation. 104:2228–2235. 2001. View Article : Google Scholar : PubMed/NCBI

33 

Cascino T, Csanyi G, Al Ghouleh I, et al: Adventitia-derived hydrogen peroxide impairs relaxation of the rat carotid artery via smooth muscle cell p38 mitogen-activated protein kinase. Antioxid Redox Signal. 15:1507–1515. 2011. View Article : Google Scholar :

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu CF, Zhang J, Shen K, Gao PJ, Wang HY, Jin X, Meng C and Fang NY: Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling. Mol Med Rep 11: 2608-2614, 2015.
APA
Liu, C., Zhang, J., Shen, K., Gao, P., Wang, H., Jin, X. ... Fang, N. (2015). Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling. Molecular Medicine Reports, 11, 2608-2614. https://doi.org/10.3892/mmr.2014.3069
MLA
Liu, C., Zhang, J., Shen, K., Gao, P., Wang, H., Jin, X., Meng, C., Fang, N."Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling". Molecular Medicine Reports 11.4 (2015): 2608-2614.
Chicago
Liu, C., Zhang, J., Shen, K., Gao, P., Wang, H., Jin, X., Meng, C., Fang, N."Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling". Molecular Medicine Reports 11, no. 4 (2015): 2608-2614. https://doi.org/10.3892/mmr.2014.3069
Copy and paste a formatted citation
x
Spandidos Publications style
Liu CF, Zhang J, Shen K, Gao PJ, Wang HY, Jin X, Meng C and Fang NY: Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling. Mol Med Rep 11: 2608-2614, 2015.
APA
Liu, C., Zhang, J., Shen, K., Gao, P., Wang, H., Jin, X. ... Fang, N. (2015). Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling. Molecular Medicine Reports, 11, 2608-2614. https://doi.org/10.3892/mmr.2014.3069
MLA
Liu, C., Zhang, J., Shen, K., Gao, P., Wang, H., Jin, X., Meng, C., Fang, N."Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling". Molecular Medicine Reports 11.4 (2015): 2608-2614.
Chicago
Liu, C., Zhang, J., Shen, K., Gao, P., Wang, H., Jin, X., Meng, C., Fang, N."Adventitial gene transfer of catalase attenuates angiotensin II-induced vascular remodeling". Molecular Medicine Reports 11, no. 4 (2015): 2608-2614. https://doi.org/10.3892/mmr.2014.3069
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team