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Article

Mycobacterium tuberculosis 19-kDa lipoprotein induces Toll-like receptor 2-dependent peroxisome proliferator-activated receptor γ expression and promotes inflammatory responses in human macrophages

  • Authors:
    • Li Liu
    • Jincheng Liu
    • Guoqiang Niu
    • Qianhong Xu
    • Qiliang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Tuberculosis Medicine, The Tuberculosis Prevention and Care Hospital of Shaanxi Province, Xi'an, Shaanxi 710100, P.R. China
  • Pages: 2921-2926
    |
    Published online on: December 10, 2014
       https://doi.org/10.3892/mmr.2014.3070
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Abstract

Mycobacterium tuberculosis (M.tb) enhances its survival in macrophages by suppressing immune responses, in part through its complex cell wall structures. M.tb 19‑kDa lipoprotein (P19), a component of the complex cell wall structures of M.tb, is a Toll‑like receptor (TLR) agonist, and may induce immune responses through TLR2. Furthermore, the activation of peroxisome proliferator‑activated receptor γ (PPARγ) is also involved in M.tb‑induced immune responses in macrophages. In the present study, specific agonists/antagonists and siRNA were used to investigate the role of PPARγ in P19‑induced immune responses in human macrophages, including TLR2 activation, p38 phosphorylation and cytokine production. In the present study, PPARγ expression, p38 phosphorylation and cytokine production were upregulated following M.tb H37Rv infection or P19 treatment. By pretreating macrophages with a specific PPARγ agonist or antagonist, it was demonstrated that phosphorylation and IL‑6 production are modulated in macrophages by PPARγ activity. Following TLR2 knockdown in macrophages, the expression of PPARγ was significantly decreased in the presence or absence of P19 treatment. Furthermore, p38 phosphorylation and cytokine production were significantly reduced in TLR2 knockdown macrophages following P19 treatment. It was demonstrated in the current study that PPARγ was induced and activated by M.tb infection and that P19‑induced PPARγ expression, p38 phosphorylation and cytokine production in macrophages are dependent on TLR2. These findings suggest a role for PPARγ and TLR2 in P19‑induced p38 phosphorylation and cytokine production, thereby potentially influencing M.tb pathogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Liu L, Liu J, Niu G, Xu Q and Chen Q: Mycobacterium tuberculosis 19-kDa lipoprotein induces Toll-like receptor 2-dependent peroxisome proliferator-activated receptor γ expression and promotes inflammatory responses in human macrophages. Mol Med Rep 11: 2921-2926, 2015.
APA
Liu, L., Liu, J., Niu, G., Xu, Q., & Chen, Q. (2015). Mycobacterium tuberculosis 19-kDa lipoprotein induces Toll-like receptor 2-dependent peroxisome proliferator-activated receptor γ expression and promotes inflammatory responses in human macrophages. Molecular Medicine Reports, 11, 2921-2926. https://doi.org/10.3892/mmr.2014.3070
MLA
Liu, L., Liu, J., Niu, G., Xu, Q., Chen, Q."Mycobacterium tuberculosis 19-kDa lipoprotein induces Toll-like receptor 2-dependent peroxisome proliferator-activated receptor γ expression and promotes inflammatory responses in human macrophages". Molecular Medicine Reports 11.4 (2015): 2921-2926.
Chicago
Liu, L., Liu, J., Niu, G., Xu, Q., Chen, Q."Mycobacterium tuberculosis 19-kDa lipoprotein induces Toll-like receptor 2-dependent peroxisome proliferator-activated receptor γ expression and promotes inflammatory responses in human macrophages". Molecular Medicine Reports 11, no. 4 (2015): 2921-2926. https://doi.org/10.3892/mmr.2014.3070
Copy and paste a formatted citation
x
Spandidos Publications style
Liu L, Liu J, Niu G, Xu Q and Chen Q: Mycobacterium tuberculosis 19-kDa lipoprotein induces Toll-like receptor 2-dependent peroxisome proliferator-activated receptor γ expression and promotes inflammatory responses in human macrophages. Mol Med Rep 11: 2921-2926, 2015.
APA
Liu, L., Liu, J., Niu, G., Xu, Q., & Chen, Q. (2015). Mycobacterium tuberculosis 19-kDa lipoprotein induces Toll-like receptor 2-dependent peroxisome proliferator-activated receptor γ expression and promotes inflammatory responses in human macrophages. Molecular Medicine Reports, 11, 2921-2926. https://doi.org/10.3892/mmr.2014.3070
MLA
Liu, L., Liu, J., Niu, G., Xu, Q., Chen, Q."Mycobacterium tuberculosis 19-kDa lipoprotein induces Toll-like receptor 2-dependent peroxisome proliferator-activated receptor γ expression and promotes inflammatory responses in human macrophages". Molecular Medicine Reports 11.4 (2015): 2921-2926.
Chicago
Liu, L., Liu, J., Niu, G., Xu, Q., Chen, Q."Mycobacterium tuberculosis 19-kDa lipoprotein induces Toll-like receptor 2-dependent peroxisome proliferator-activated receptor γ expression and promotes inflammatory responses in human macrophages". Molecular Medicine Reports 11, no. 4 (2015): 2921-2926. https://doi.org/10.3892/mmr.2014.3070
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