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Article

Histone deacetylase inhibitor suberoyl bis-hydroxamic acid suppresses cell proliferation and induces apoptosis in breast cancer cells

  • Authors:
    • Xinmiao Yang
    • Ning Zhang
    • Zeliang Shi
    • Zhangyu Yang
    • Xichun Hu
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, Minhang Branch of Fudan University Shanghai Cancer Center, Shanghai 200240, P.R. China, Department of Radiation Oncology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, P.R. China, Department of Medical Oncology, Fudan University Cancer Hospital, Shanghai 200032, P.R. China
  • Pages: 2908-2912
    |
    Published online on: December 11, 2014
       https://doi.org/10.3892/mmr.2014.3076
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Abstract

Suberoyl bis‑hydroxamic acid (SBHA) is a histone deacetylase inhibitor that has shown anticancer activity against numerous types of human cancer. The aim of the current study was to explore the effects of SBHA on the proliferation and apoptosis of breast cancer cells. MCF‑7 breast cancer cells were treated with different concentrations of SBHA and tested for cell viability, apoptosis and gene expression changes. The results showed that SBHA significantly inhibited the proliferation of MCF‑7 cells in a concentration‑dependent manner, as determined using a Cell Counting kit‑8 assay. SBHA‑treated MCF‑7 cells showed G0/G1 cell‑cycle arrest, coupled with elevated expression levels of p21 and p27 proteins. Hoechst 33258 staining revealed cell shrinkage, chromosomal condensation and nuclear fragmentation in MCF‑7 cells treated with SBHA. Flow cytometric analysis of Annexin V‑stained cells showed that SBHA treatment induced apoptotic cell death in a concentration‑dependent manner. Western blot analysis confirmed the upregulation of Bax and the downregulation of Bcl‑2 by SBHA. In conclusion, these results indicate that SBHA exerts cytotoxic effects against human breast cancer cells, which involves the modulation of p21, p27 and Bcl‑2 family proteins, consequently leading to cell‑cycle arrest and apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Zhang N, Shi Z, Yang Z and Hu X: Histone deacetylase inhibitor suberoyl bis-hydroxamic acid suppresses cell proliferation and induces apoptosis in breast cancer cells. Mol Med Rep 11: 2908-2912, 2015.
APA
Yang, X., Zhang, N., Shi, Z., Yang, Z., & Hu, X. (2015). Histone deacetylase inhibitor suberoyl bis-hydroxamic acid suppresses cell proliferation and induces apoptosis in breast cancer cells. Molecular Medicine Reports, 11, 2908-2912. https://doi.org/10.3892/mmr.2014.3076
MLA
Yang, X., Zhang, N., Shi, Z., Yang, Z., Hu, X."Histone deacetylase inhibitor suberoyl bis-hydroxamic acid suppresses cell proliferation and induces apoptosis in breast cancer cells". Molecular Medicine Reports 11.4 (2015): 2908-2912.
Chicago
Yang, X., Zhang, N., Shi, Z., Yang, Z., Hu, X."Histone deacetylase inhibitor suberoyl bis-hydroxamic acid suppresses cell proliferation and induces apoptosis in breast cancer cells". Molecular Medicine Reports 11, no. 4 (2015): 2908-2912. https://doi.org/10.3892/mmr.2014.3076
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Zhang N, Shi Z, Yang Z and Hu X: Histone deacetylase inhibitor suberoyl bis-hydroxamic acid suppresses cell proliferation and induces apoptosis in breast cancer cells. Mol Med Rep 11: 2908-2912, 2015.
APA
Yang, X., Zhang, N., Shi, Z., Yang, Z., & Hu, X. (2015). Histone deacetylase inhibitor suberoyl bis-hydroxamic acid suppresses cell proliferation and induces apoptosis in breast cancer cells. Molecular Medicine Reports, 11, 2908-2912. https://doi.org/10.3892/mmr.2014.3076
MLA
Yang, X., Zhang, N., Shi, Z., Yang, Z., Hu, X."Histone deacetylase inhibitor suberoyl bis-hydroxamic acid suppresses cell proliferation and induces apoptosis in breast cancer cells". Molecular Medicine Reports 11.4 (2015): 2908-2912.
Chicago
Yang, X., Zhang, N., Shi, Z., Yang, Z., Hu, X."Histone deacetylase inhibitor suberoyl bis-hydroxamic acid suppresses cell proliferation and induces apoptosis in breast cancer cells". Molecular Medicine Reports 11, no. 4 (2015): 2908-2912. https://doi.org/10.3892/mmr.2014.3076
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