Curcumin and its major metabolites inhibit the inflammatory response induced by lipopolysaccharide: Translocation of nuclear factor-κB as potential target

Zhao,Feng; Gong,Yudian; Hu,Yuan; Lu,Minghui; Wang,Jing; Dong,Jianxin; Chen,Daquan; Chen,Lei; Fu,Fenghua; Qiu,Feng

doi:10.3892/mmr.2014.3079

Curcumin and its major metabolites inhibit the inflammatory response induced by lipopolysaccharide: Translocation of nuclear factor-κB as potential target

Authors:
- Feng Zhao
- Yudian Gong
- Yuan Hu
- Minghui Lu
- Jing Wang
- Jianxin Dong
- Daquan Chen
- Lei Chen
- Fenghua Fu
- Feng Qiu
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Affiliations: Department of Natural Products Chemistry, School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China, Department of Clinical Laboratory, Yantai Psychological Rehabilitation Hospital, Yantai, Shandong 265200, P.R. China, Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P.R. China
Published online on: December 11, 2014 https://doi.org/10.3892/mmr.2014.3079
Pages: 3087-3093

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Abstract

The aim of the present study was to investigate and compare the anti‑inflammatory activities of curcumin and its three metabolites, tetrahydrocurcumin, hexahydrocurcumin and octahydrocurcumin in lipopolysaccharide (LPS)‑stimulated RAW 264.7 macrophage cells. The results demonstrated that overproduction of nitric oxide (NO) was potently inhibited following treatment with curcumin and its three metabolites. In addition, curcumin and tetrahydrocurcumin significantly inhibited the release of prominent cytokines, including tumor necrosis factor‑α (TNF‑α) and interleukin‑6 (IL‑6); however, hexahydrocurcumin and octahydrocurcumin did not significantly alter cytokine release. Furthermore, the present study investigated the effect of curcumin and its metabolites on the expression of inducible NO synthase (iNOS), cyclooxygenase‑2 (COX‑2) and activated‑nuclear factor kappa B (NF‑κB); the results showed that curcumin and its three metabolites significantly inhibited LPS‑mediated upregulation of iNOS and COX‑2 as well as NF‑κB activation. However, curcumin exerted a more potent effect on LPS‑stimulated RAW 264.7 cells compared to that of its three metabolites, of which tetrahydrocurcuim was found to be the most pharmacologically active. In conclusion, the results of the present study demonstrated that curcumin and its major metabolites inhibited the LPS‑induced inflammatory response via the mechanism of inhibiting NF‑κB translocation to the nucleus.

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