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Article

Iodine-131 induces apoptosis in HTori-3 human thyrocyte cell line and G2/M phase arrest in a p53-independent pathway

  • Authors:
    • Weixiao Zhang
    • Rui Gao
    • Yan Yu
    • Kun Guo
    • Peng  Hou
    • Mingqi Yu
    • Yan Liu
    • Aimin Yang
  • View Affiliations / Copyright

    Affiliations: Department of Nuclear Medicine, The First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China, Department of Public Health, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China, Endocrinology Laboratory, The First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 3148-3154
    |
    Published online on: December 16, 2014
       https://doi.org/10.3892/mmr.2014.3096
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Abstract

Iodine‑131 is known to destroy residual thyroid tissue following surgical resection of differentiated thyroid carcinoma and is widely used to treat hyperthyroidism. However, the mechanism by which iodine‑131 induces apoptosis and cell cycle arrest in the human thyrocyte cell line, Htori‑3, remains to be elucidated. In the present study, the cytotoxic effect of iodine‑131 on the HTori‑3 cell line and the underlying mechanism of iodine‑131‑induced cell apoptosis were investigated. Cell viability was analyzed using an MTT assay, while cell apoptosis and cell cycle arrest were determined using flow cytometry. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analyses were performed to determine the changes in the expression levels of p53, B‑cell lymphoma 2 (Bcl‑2), Fas and growth arrest and DNA damage‑inducible 45 (GADD45), following iodine‑131 treatment. The results demonstrated that iodine‑131 may inhibit HTori‑3 cell growth via cell apoptosis and G2/M phase arrest in a time‑ and dose‑dependent manner. The iodine‑131 dose required for 50% growth inhibition of HTori‑3 cell viability 48 h after treatment was 27.75±2.22 MBq/ml. Upregulation of Fas and downregulation of Bcl‑2 expression levels were observed following iodine‑131 treatment. The results of RT‑qPCR revealed an increase in the GADD45 mRNA expression following HTori‑3 cell exposure to iodine‑131. Notably, the mRNA and protein expression levels of p53 were not altered following iodine‑131 treatment. In conclusion, iodine‑131 may induce apoptosis in HTori‑3 cells by downregulating the expression of Bcl‑2 and upregulating the expression of Fas. In addition, iodine‑131 may upregulate GADD45 mRNA expression in HTori‑3 cells, resulting in G2/M phase arrest in a p53‑independent pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang W, Gao R, Yu Y, Guo K, Hou P, Yu M, Liu Y and Yang A: Iodine-131 induces apoptosis in HTori-3 human thyrocyte cell line and G2/M phase arrest in a p53-independent pathway. Mol Med Rep 11: 3148-3154, 2015.
APA
Zhang, W., Gao, R., Yu, Y., Guo, K., Hou, P., Yu, M. ... Yang, A. (2015). Iodine-131 induces apoptosis in HTori-3 human thyrocyte cell line and G2/M phase arrest in a p53-independent pathway. Molecular Medicine Reports, 11, 3148-3154. https://doi.org/10.3892/mmr.2014.3096
MLA
Zhang, W., Gao, R., Yu, Y., Guo, K., Hou, P., Yu, M., Liu, Y., Yang, A."Iodine-131 induces apoptosis in HTori-3 human thyrocyte cell line and G2/M phase arrest in a p53-independent pathway". Molecular Medicine Reports 11.4 (2015): 3148-3154.
Chicago
Zhang, W., Gao, R., Yu, Y., Guo, K., Hou, P., Yu, M., Liu, Y., Yang, A."Iodine-131 induces apoptosis in HTori-3 human thyrocyte cell line and G2/M phase arrest in a p53-independent pathway". Molecular Medicine Reports 11, no. 4 (2015): 3148-3154. https://doi.org/10.3892/mmr.2014.3096
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang W, Gao R, Yu Y, Guo K, Hou P, Yu M, Liu Y and Yang A: Iodine-131 induces apoptosis in HTori-3 human thyrocyte cell line and G2/M phase arrest in a p53-independent pathway. Mol Med Rep 11: 3148-3154, 2015.
APA
Zhang, W., Gao, R., Yu, Y., Guo, K., Hou, P., Yu, M. ... Yang, A. (2015). Iodine-131 induces apoptosis in HTori-3 human thyrocyte cell line and G2/M phase arrest in a p53-independent pathway. Molecular Medicine Reports, 11, 3148-3154. https://doi.org/10.3892/mmr.2014.3096
MLA
Zhang, W., Gao, R., Yu, Y., Guo, K., Hou, P., Yu, M., Liu, Y., Yang, A."Iodine-131 induces apoptosis in HTori-3 human thyrocyte cell line and G2/M phase arrest in a p53-independent pathway". Molecular Medicine Reports 11.4 (2015): 3148-3154.
Chicago
Zhang, W., Gao, R., Yu, Y., Guo, K., Hou, P., Yu, M., Liu, Y., Yang, A."Iodine-131 induces apoptosis in HTori-3 human thyrocyte cell line and G2/M phase arrest in a p53-independent pathway". Molecular Medicine Reports 11, no. 4 (2015): 3148-3154. https://doi.org/10.3892/mmr.2014.3096
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