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Article

Glucocorticoids induce autophagy in rat bone marrow mesenchymal stem cells

  • Authors:
    • Long Wang
    • Jing Fan
    • Yan‑Shui Lin
    • Yun‑Shan Guo
    • Bo Gao
    • Qi‑Yue  Shi
    • Bo‑Yuan Wei
    • Li Chen
    • Liu Yang
    • Jian Liu
    • Zhuo‑Jing Luo
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Orthopedics, First Affiliated Hospital, Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China, Laboratory of Molecular Endocrinology, Odense University Hospital, University of Southern Denmark, 5000 Odense C, Denmark
  • Pages: 2711-2716
    |
    Published online on: December 16, 2014
       https://doi.org/10.3892/mmr.2014.3099
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Abstract

Glucocorticoid‑induced osteoporosis (GIOP) is a widespread clinical complication following glucocorticoid therapy. This irreversible damage to bone‑forming and ‑resorbing cells is essential in the pathogenesis of osteoporosis. Autophagy is a physiological process involved in the regulation of cells and their responses to diverse stimuli, however, the role of autophagy in glucocorticoid‑induced damage to bone marrow mesenchymal stem cells (BMSCs) remains unclear. The current study confirmed that glucocorticoid administration impaired the proliferation of BMSCs. Transmission electron microscopy, immunohistochemistry and western blot analysis detected autophagy in vitro and in GIOP model rats (in vivo). With the addition of the autophagy inhibitor 3‑methyladenine, the proliferative ability of BMSCs was further reduced, while the number of apoptotic BMSCs was significantly increased. The data suggests that in response to glucocorticoid administration, induced autophagy aids to maintain proliferation and prevent apoptosis of BMSCs. Thus, it is hypothesized that autophagy may be a novel target in the treatment or prevention of osteoporosis.
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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Fan J, Lin YS, Guo YS, Gao B, Shi QY, Wei BY, Chen L, Yang L, Liu J, Liu J, et al: Glucocorticoids induce autophagy in rat bone marrow mesenchymal stem cells. Mol Med Rep 11: 2711-2716, 2015.
APA
Wang, L., Fan, J., Lin, Y., Guo, Y., Gao, B., Shi, Q. ... Luo, Z. (2015). Glucocorticoids induce autophagy in rat bone marrow mesenchymal stem cells. Molecular Medicine Reports, 11, 2711-2716. https://doi.org/10.3892/mmr.2014.3099
MLA
Wang, L., Fan, J., Lin, Y., Guo, Y., Gao, B., Shi, Q., Wei, B., Chen, L., Yang, L., Liu, J., Luo, Z."Glucocorticoids induce autophagy in rat bone marrow mesenchymal stem cells". Molecular Medicine Reports 11.4 (2015): 2711-2716.
Chicago
Wang, L., Fan, J., Lin, Y., Guo, Y., Gao, B., Shi, Q., Wei, B., Chen, L., Yang, L., Liu, J., Luo, Z."Glucocorticoids induce autophagy in rat bone marrow mesenchymal stem cells". Molecular Medicine Reports 11, no. 4 (2015): 2711-2716. https://doi.org/10.3892/mmr.2014.3099
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Fan J, Lin YS, Guo YS, Gao B, Shi QY, Wei BY, Chen L, Yang L, Liu J, Liu J, et al: Glucocorticoids induce autophagy in rat bone marrow mesenchymal stem cells. Mol Med Rep 11: 2711-2716, 2015.
APA
Wang, L., Fan, J., Lin, Y., Guo, Y., Gao, B., Shi, Q. ... Luo, Z. (2015). Glucocorticoids induce autophagy in rat bone marrow mesenchymal stem cells. Molecular Medicine Reports, 11, 2711-2716. https://doi.org/10.3892/mmr.2014.3099
MLA
Wang, L., Fan, J., Lin, Y., Guo, Y., Gao, B., Shi, Q., Wei, B., Chen, L., Yang, L., Liu, J., Luo, Z."Glucocorticoids induce autophagy in rat bone marrow mesenchymal stem cells". Molecular Medicine Reports 11.4 (2015): 2711-2716.
Chicago
Wang, L., Fan, J., Lin, Y., Guo, Y., Gao, B., Shi, Q., Wei, B., Chen, L., Yang, L., Liu, J., Luo, Z."Glucocorticoids induce autophagy in rat bone marrow mesenchymal stem cells". Molecular Medicine Reports 11, no. 4 (2015): 2711-2716. https://doi.org/10.3892/mmr.2014.3099
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