Resveratrol induces cell cycle arrest via a p53-independent pathway in A549 cells

Yuan,Long; Zhang,Yongrong; Xia,Juan; Liu,Bin; Zhang,Qingyu; Liu,Jie; Luo,Liming; Peng,Zhou; Song,Zeqing; Zhu,Runzhi

doi:10.3892/mmr.2014.3100

April-2015 Volume 11 Issue 4

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April-2015 Volume 11 Issue 4

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Article Open Access

Resveratrol induces cell cycle arrest via a p53-independent pathway in A549 cells

Authors:
- Long Yuan
- Yongrong Zhang
- Juan Xia
- Bin Liu
- Qingyu Zhang
- Jie Liu
- Liming Luo
- Zhou Peng
- Zeqing Song
- Runzhi Zhu
View Affiliations / Copyright

Affiliations: Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China, Laboratory of Hepatobiliary Surgery, Zhanjiang Key Laboratory of Hepatobiliary Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China, Department of Respiratory Medicine, The Fourth Hospital of Zhanjiang, Zhanjiang, Guangdong 524001, P.R. China

Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 2459-2464
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Published online on: December 16, 2014

https://doi.org/10.3892/mmr.2014.3100
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Abstract

Resveratrol, a non‑flavone polyphenol compound, has a chemopreventive and chemotherapeutic effect against the progression of multiple types of cancer, including lung cancer. However, the molecular mechanism underlying the effects of resveratrol on cancer remain to be elucidated. In the present study, using an MTT assay, it was demonstrated that resveratrol inhibited cell proliferation in a concentration‑ and time‑dependent manner. In addition, morphological features were observed in the A549, human lung cancer cell line, which included cell shrinkage, cells became distorted, certain cells became rounded and there was a concentration‑dependent increase in the number of sloughed cells. Cell cycle analysis revealed that resveratrol may induce cell cycle arrest in the G0/G1 phase by downregulating the expression levels of cyclin D1, cyclin‑dependent kinase (CDK)4 and CDK6, and upregulating the expression levels of the CDK inhibitors, p21 and p27. The immunofluorescence and western blot analysis results revealed that resveratrol upregulated the nuclear expression of p53 in A549 cells. Further studies have demonstrated that p53 downregulation did not contribute to the G0/G1 cell cycle arrest induced by resveratrol. In addition, resveratrol had no effect on the expression of p21, through use of the p53 inhibitor, pifithrin‑α. The present study may offer a scientific basis for the further in‑depth evaluation of resveratrol in the association of p53 and cell cycle arrest.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Resveratrol induces cell cycle arrest via a p53-independent pathway in A549 cells

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