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Article

RPL13A as a reference gene for normalizing mRNA transcription of ovarian cancer cells with paclitaxel and 10-hydroxycamptothecin treatments

  • Authors:
    • Zehua Bian
    • Yang Yu
    • Chao Quan
    • Rongwei Guan
    • Yan Jin
    • Jie  Wu
    • Lidan Xu
    • Feng Chen
    • Jing Bai
    • Wenjing Sun
    • Songbin Fu
  • View Affiliations / Copyright

    Affiliations: Laboratory of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China
  • Pages: 3188-3194
    |
    Published online on: December 17, 2014
       https://doi.org/10.3892/mmr.2014.3108
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Abstract

Gene transcription analysis is important in cancer research, and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) has been demonstrated to be an effective method to evaluate gene transcription in cancer. RT‑qPCR requires an internal reference gene with a consistent level of mRNA transcription across various experimental conditions. However, it has been suggested that different treatments, including anticancer therapy, may influence the transcriptional stability of internal reference genes. Paclitaxel (PTX) and 10‑hydroxycamptothecin (HCPT) are widely used to treat various types of cancer, and a suitable internal reference gene is required in order to analyze the transcription profiles of the cells following treatment. In the current study, the transcriptional stability of 30 candidate reference genes was investigated in cancer cells following treatment with PTX and HCPT. The two ovarian cancer cell lines, UACC‑1598 and SKOV3, were treated with PTX and HCPT for 24 and 48 h, and the transcriptional levels of the candidate reference genes were subsequently evaluated by RT‑qPCR analysis. The transcriptional stability of the selected genes was then analyzed using qbase+ and NormFinder software. A total of 9 genes were demonstrated to exhibit high transcriptional stability and one of these genes, ribosomal protein L13a (RPL13A), was identified to exhibit high transcriptional stability in every group. The current study identified various reference genes suitable under different circumstances, while RPL13A was indicated to be the most suitable reference gene for analyzing the transcription profile of ovarian cancer cells following treatment with PTX and HCPT.
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Copy and paste a formatted citation
Spandidos Publications style
Bian Z, Yu Y, Quan C, Guan R, Jin Y, Wu J, Xu L, Chen F, Bai J, Sun W, Sun W, et al: RPL13A as a reference gene for normalizing mRNA transcription of ovarian cancer cells with paclitaxel and 10-hydroxycamptothecin treatments. Mol Med Rep 11: 3188-3194, 2015.
APA
Bian, Z., Yu, Y., Quan, C., Guan, R., Jin, Y., Wu, J. ... Fu, S. (2015). RPL13A as a reference gene for normalizing mRNA transcription of ovarian cancer cells with paclitaxel and 10-hydroxycamptothecin treatments. Molecular Medicine Reports, 11, 3188-3194. https://doi.org/10.3892/mmr.2014.3108
MLA
Bian, Z., Yu, Y., Quan, C., Guan, R., Jin, Y., Wu, J., Xu, L., Chen, F., Bai, J., Sun, W., Fu, S."RPL13A as a reference gene for normalizing mRNA transcription of ovarian cancer cells with paclitaxel and 10-hydroxycamptothecin treatments". Molecular Medicine Reports 11.4 (2015): 3188-3194.
Chicago
Bian, Z., Yu, Y., Quan, C., Guan, R., Jin, Y., Wu, J., Xu, L., Chen, F., Bai, J., Sun, W., Fu, S."RPL13A as a reference gene for normalizing mRNA transcription of ovarian cancer cells with paclitaxel and 10-hydroxycamptothecin treatments". Molecular Medicine Reports 11, no. 4 (2015): 3188-3194. https://doi.org/10.3892/mmr.2014.3108
Copy and paste a formatted citation
x
Spandidos Publications style
Bian Z, Yu Y, Quan C, Guan R, Jin Y, Wu J, Xu L, Chen F, Bai J, Sun W, Sun W, et al: RPL13A as a reference gene for normalizing mRNA transcription of ovarian cancer cells with paclitaxel and 10-hydroxycamptothecin treatments. Mol Med Rep 11: 3188-3194, 2015.
APA
Bian, Z., Yu, Y., Quan, C., Guan, R., Jin, Y., Wu, J. ... Fu, S. (2015). RPL13A as a reference gene for normalizing mRNA transcription of ovarian cancer cells with paclitaxel and 10-hydroxycamptothecin treatments. Molecular Medicine Reports, 11, 3188-3194. https://doi.org/10.3892/mmr.2014.3108
MLA
Bian, Z., Yu, Y., Quan, C., Guan, R., Jin, Y., Wu, J., Xu, L., Chen, F., Bai, J., Sun, W., Fu, S."RPL13A as a reference gene for normalizing mRNA transcription of ovarian cancer cells with paclitaxel and 10-hydroxycamptothecin treatments". Molecular Medicine Reports 11.4 (2015): 3188-3194.
Chicago
Bian, Z., Yu, Y., Quan, C., Guan, R., Jin, Y., Wu, J., Xu, L., Chen, F., Bai, J., Sun, W., Fu, S."RPL13A as a reference gene for normalizing mRNA transcription of ovarian cancer cells with paclitaxel and 10-hydroxycamptothecin treatments". Molecular Medicine Reports 11, no. 4 (2015): 3188-3194. https://doi.org/10.3892/mmr.2014.3108
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