Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
April-2015 Volume 11 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2015 Volume 11 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Suppressive effects of induced pluripotent stem cell-conditioned medium on in vitro hypertrophic scarring fibroblast activation

  • Authors:
    • Ye Ren
    • Chen‑Liang Deng
    • Wei‑Dong Wan
    • Jiang‑Hong  Zheng
    • Guang‑Yu  Mao
    • Song‑Lin  Yang
  • View Affiliations / Copyright

    Affiliations: Department of Plastic Surgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
    Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2471-2476
    |
    Published online on: December 18, 2014
       https://doi.org/10.3892/mmr.2014.3115
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Hypertrophic scarring (HS) is a type of fibrosis that occurs in the skin, and is characterized by fibroblast activation and excessive collagen production. However, at present, therapeutic strategies for this condition are ineffective. Previous studies have identified that the mutual regulation of chronic inflammation, mechanical force and fibroblast activation leads to the formation of HS. Induced pluripotent stem cells (iPSCs) are novel bioengineered embryonic‑like stem cells, initially created from mouse adult fibroblasts. The current study demonstrated that iPSC‑conditioned medium (iPSC‑CM) may significantly suppress hypertrophic scar fibroblast activation. It was observed that in the presence of iPSC‑CM, the level of collagen I was markedly reduced and α‑smooth muscle actin, a marker for myofibroblasts (activated fibroblasts that mediate mechanical force‑induced HS formation), exhibited a significantly lower level of expression in human dermal fibroblasts (HDFs) activated with transforming growth factor‑β1. Additionally, iPSC‑CM attenuated the local inflammatory cell response by blocking the adhesion of human acute monocytic leukemia cell monocytes and fibroblasts in vitro. In addition, the contractile ability of HDFs may be reduced by iPSC‑CM. These observations suggest that iPSC‑CM may protect against processes leading to hypertrophic scarring by attenuating fibroblast activation, blocking inflammatory cell recruitment and adhesion and reducing the contractile ability of fibroblasts.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Canady J, Arndt S, Karrer S and Bosserhoff AK: Increased KGF expression promotes fibroblast activation in a double paracrine manner resulting in cutaneous fibrosis. J Invest Dermatol. 133:647–657. 2013. View Article : Google Scholar

2 

Beanes SR, Dang C, Soo C and Ting K: Skin repair and scar formation: the central role of TGF-beta. Expert Rev Mol Med. 5:1–22. 2003. View Article : Google Scholar

3 

Clark RA: The Molecular and Cellular Biology of Wound Repair. 2nd edition. Springer; New York, NY: pp. 22–23. 1996

4 

Kovacs EJ: Fibrogenic cytokines: the role of immune mediators in the development of scar tissue. Immunol Today. 12:17–23. 1991. View Article : Google Scholar : PubMed/NCBI

5 

Takahashi K and Yamanaka S: Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 126:663–676. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Neel S and Singla DK: Induced pluripotent stem (iPS) cells inhibit apoptosis and fibrosis in streptozotocin-induced diabetic rats. Mol Pharm. 8:2350–2357. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Singla DK, Long X, Glass C, Singla RD and Yan B: Induced pluripotent stem (iPS) cells repair and regenerate infarcted myocardium. Mol Pharm. 8:1573–1581. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Yan Q, Quan Y, Sun H, et al: A site-specific genetic modification for induction of pluripotency and subsequent isolation of derived lung alveolar epithelial type II cells. Stem Cells. 32:402–413. 2014. View Article : Google Scholar :

9 

Chou YH, Pan SY, Yang CH and Lin SL: Stem cells and kidney regeneration. J Formos Med Assoc. 113:201–209. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Colman A and Dreesen O: Induced pluripotent stem cells and the stability of the differentiated state. EMBO Rep. 10:714–721. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Li LF, Liu YY, Yang CT, et al: Improvement of ventilator-induced lung injury by IPS cell-derived conditioned medium via inhibition of PI3K/Akt pathway and IP-10-dependent paracrine regulation. Biomaterials. 34:78–91. 2013. View Article : Google Scholar

12 

Chen SJ, Chang CM, Tsai SK, et al: Functional improvement of focal cerebral ischemia injury by subdural transplantation of induced pluripotent stem cells with fibrin glue. Stem Cells Dev. 19:1757–1767. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Xu MJ, Liu XJ, Zhao YL, et al: Identification and characterization of an anti-fibrotic benzopyran compound isolated from mangrove-derived Streptomyces xiamenensis. Mar Drugs. 10:639–654. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Zhang ZG, Bothe I, Hirche F, et al: Interactions of primary fibroblasts and keratinocytes with extracellular matrix proteins: contribution of alpha2beta1 integrin. J Cell Sci. 119:1886–1895. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Mun JH, Kim YM, Kim BS, Kim JH, Kim MB and Ko HC: Simvastatin inhibits transforming growth factor-β1-induced expression of type I collagen, CTGF, and α-SMA in keloid fibroblasts. Wound Repair Regen. 22:125–133. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Liu XJ, Xu MJ, Fan ST, et al: Xiamenmycin attenuates hypertrophic scars by suppressing local inflammation and the effects of mechanical stress. J Invest Dermatol. 133:1351–1360. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Sarrazy V, Billet F, Micallef L, Coulomb B and Desmoulière A: Mechanisms of pathological scarring: role of myofibroblasts and current developments. Wound Repair Regen. 19(Suppl 1): s10–s15. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Li M, Riddle SR, Frid MG, et al: Emergence of fibroblasts with a proinflammatory epigenetically altered phenotype in severe hypoxic pulmonary hypertension. J Immunol. 187:2711–2722. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Chung AS and Kao WJ: Fibroblasts regulate monocyte response to ECM-derived matrix: the effects on monocyte adhesion and the production of inflammatory, matrix remodeling, and growth factor proteins. J Biomed Mater Res A. 89:841–853. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Ngo P, Ramalingam P, Phillips JA and Furuta GT: Collagen gel contraction assay. Methods Mol Biol. 341:103–109. 2006.PubMed/NCBI

21 

Wong VW, Paterno J, Sorkin M, et al: Mechanical force prolongs acute inflammation via T-cell-dependent pathways during scar formation. FASEB J. 25:4498–4510. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Shaker SA, Ayuob NN and Hajrah NH: Cell talk: a phenomenon observed in the keloid scar by immunohistochemical study. Appl Immunohistochem Mol Morphol. 19:153–159. 2011. View Article : Google Scholar

23 

Wong VW, Rustad KC, Akaishi S, et al: Focal adhesion kinase links mechanical force to skin fibrosis via inflammatory signaling. Nat Med. 18:148–152. 2011. View Article : Google Scholar : PubMed/NCBI

24 

van der Veer WM, Bloemen MC, Ulrich MM, et al: Potential cellular and molecular causes of hypertrophic scar formation. Burns. 35:15–29. 2009. View Article : Google Scholar

25 

Clayton A, Evans RA, Pettit E, Hallett M, Williams JD and Steadman R: Cellular activation through the ligation of intercellular adhesion molecule-1. J Cell Sci. 111:443–453. 1998.PubMed/NCBI

26 

Agarwal C, Britton ZT, Alaseirlis DA, Li Y and Wang JH: Healing and normal fibroblasts exhibit differential proliferation, collagen production, alpha-SMA expression, and contraction. Ann Biomed Eng. 34:653–659. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Darby I, Skalli O and Gabbiani G: Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing. Lab Invest. 63:21–29. 1990.PubMed/NCBI

28 

Wang J, Chen H, Seth A and McCulloch CA: Mechanical force regulation of myofibroblast differentiation in cardiac fibroblasts. Am J Physiol Heart Circ Physiol. 285:H1871–H1881. 2003.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ren Y, Deng CL, Wan WD, Zheng JH, Mao GY and Yang SL: Suppressive effects of induced pluripotent stem cell-conditioned medium on in vitro hypertrophic scarring fibroblast activation. Mol Med Rep 11: 2471-2476, 2015.
APA
Ren, Y., Deng, C., Wan, W., Zheng, J., Mao, G., & Yang, S. (2015). Suppressive effects of induced pluripotent stem cell-conditioned medium on in vitro hypertrophic scarring fibroblast activation. Molecular Medicine Reports, 11, 2471-2476. https://doi.org/10.3892/mmr.2014.3115
MLA
Ren, Y., Deng, C., Wan, W., Zheng, J., Mao, G., Yang, S."Suppressive effects of induced pluripotent stem cell-conditioned medium on in vitro hypertrophic scarring fibroblast activation". Molecular Medicine Reports 11.4 (2015): 2471-2476.
Chicago
Ren, Y., Deng, C., Wan, W., Zheng, J., Mao, G., Yang, S."Suppressive effects of induced pluripotent stem cell-conditioned medium on in vitro hypertrophic scarring fibroblast activation". Molecular Medicine Reports 11, no. 4 (2015): 2471-2476. https://doi.org/10.3892/mmr.2014.3115
Copy and paste a formatted citation
x
Spandidos Publications style
Ren Y, Deng CL, Wan WD, Zheng JH, Mao GY and Yang SL: Suppressive effects of induced pluripotent stem cell-conditioned medium on in vitro hypertrophic scarring fibroblast activation. Mol Med Rep 11: 2471-2476, 2015.
APA
Ren, Y., Deng, C., Wan, W., Zheng, J., Mao, G., & Yang, S. (2015). Suppressive effects of induced pluripotent stem cell-conditioned medium on in vitro hypertrophic scarring fibroblast activation. Molecular Medicine Reports, 11, 2471-2476. https://doi.org/10.3892/mmr.2014.3115
MLA
Ren, Y., Deng, C., Wan, W., Zheng, J., Mao, G., Yang, S."Suppressive effects of induced pluripotent stem cell-conditioned medium on in vitro hypertrophic scarring fibroblast activation". Molecular Medicine Reports 11.4 (2015): 2471-2476.
Chicago
Ren, Y., Deng, C., Wan, W., Zheng, J., Mao, G., Yang, S."Suppressive effects of induced pluripotent stem cell-conditioned medium on in vitro hypertrophic scarring fibroblast activation". Molecular Medicine Reports 11, no. 4 (2015): 2471-2476. https://doi.org/10.3892/mmr.2014.3115
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team