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Preventive effects of 125I seeds on benign restenosis following esophageal stent implantation in a dog model

  • Authors:
    • Zhen Gan
    • Jian Jing
    • Guangyu Zhu
    • Yonglin Qin
    • Gaojun Teng
    • Jinhe Guo
  • View Affiliations / Copyright

    Affiliations: Department of Intervention and Vascular Surgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China
    Copyright: © Gan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 3382-3390
    |
    Published online on: December 22, 2014
       https://doi.org/10.3892/mmr.2014.3130
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Abstract

The present study aimed to evaluate the effects of iodine‑125 (125I) seeds on the proliferation of primary esophageal fibroblasts in dogs, and to assess the safety and preventive efficacy of 125I seed‑pre‑loaded esophageal stents in benign restenosis following implantation. Primary fibroblasts were cultured with various 125I seed activities, which were then evaluated using cell proliferation and apoptosis assays as well as cell cycle analysis using Annexin V/propidium iodide (PI) double staining and PI staining. Prior to sacrification, animals were submitted to esophageal radiography under digital subtraction angiography. Esophageal tissues were collected and examined for macroscopic, microscopic and pathological alterations. The results demonstrated a significant and dose‑dependent inhibition of fibroblast proliferation and increased apoptosis following exposure to 125I seeds. G0/G1 fibroblast populations increased in a dose‑dependent manner following treatment with 125I seeds, in contrast to cells in S phase. Four weeks following implantation, α‑smooth muscle actin and proliferating cell nuclear antigen expression levels in the experimental group were significantly lower compared with those in the control group; in addition, eight weeks following implantation, esophageal inner diameters were increased in the experimental group. 125I seeds inhibited proliferation of dog esophageal fibroblasts via cell cycle arrest and apoptosis. In conclusion, 125I seed‑pre‑loaded esophageal stents inhibited benign hyperplasia in the upper edge of the stent to a certain extent, which relieved benign restenosis following implantation with a good safety profile.
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1 

Sharma V, Mahantshetty U, Dinshaw KA, Deshpande R and Sharma S: Palliation of advanced/recurrent esophageal carcinoma with high-dose-rate brachytherapy. Int J Radiat Oncol Biol Phys. 52:310–315. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Ghosh S, Sau S, Mitra S, Manna A and Ghosh K: Palliation of dysphagia in advanced, metastatic or recurrent carcinoma oesophagus with high dose rate intraluminal brachytherapy-an eastern Indian experience of 35 cases. J Indian Med Assoc. 110:449–452. 2012.

3 

Bhatt L, Tirmazy S and Sothi S: Intraluminal high-dose-rate brachytherapy for palliation of dysphagia in cancer of the esophagus: initial experience at a single UK center. Dis Esophagus. 26:57–60. 2013. View Article : Google Scholar

4 

Song HY, Do YS, Han YM, et al: Covered, expandable esophageal metallic stent tubes: experiences in 119 patients. Radiology. 193:689–695. 1994. View Article : Google Scholar : PubMed/NCBI

5 

Dua KS, Vleggaar FP, Santharam R and Siersema PD: Removable self-expanding plastic esophageal stent as a continuous, non-permanent dilator in treating refractory benign esophageal strictures: a prospective two-center study. Am J Gastroenterol. 103:2988–2994. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Sreedharan A, Harris K, Crellin A, Forman D and Everett SM: Interventions for dysphagia in oesophageal cancer. Cochrane Database Syst Rev. CD0050482009.PubMed/NCBI

7 

Ernst A, Feller-Kopman D, Becker HD and Mehta AC: Central airway obstruction. Am J Respir Crit Care Med. 169:1278–1297. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Krueger KD, Mitra AK, DelCore MG, Hunter WJ III and Agrawal DK: A comparison of stent-induced stenosis in coronary and peripheral arteries. J Clin Pathol. 59:575–579. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Bauriedel G, Skowasch D, Jabs A, et al: Insights into vascular pathology after intracoronary brachytherapy. Z Kardiol. 91(Suppl 3): 1–9. 2002. View Article : Google Scholar

10 

Guo JH, Teng GJ, Zhu GY, et al: Self-expandable esophageal stent loaded with 125I seeds: initial experience in patients with advanced esophageal cancer. Radiology. 247:574–581. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Guo JH, Teng GJ, Zhu GY, He SC, Deng G and He J: Self-expandable stent loaded with 125I seeds: feasibility and safety in a rabbit model. Eur J Radiol. 61:356–361. 2007. View Article : Google Scholar

12 

Mayoral W, Fleischer D, Salcedo J, Roy P, Al-Kawas F and Benjamin S: Nonmalignant obstruction is a common problem with metal stents in the treatment of esophageal cancer. Gastrointest Endosc. 51:556–559. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Kim JH, Song HY, Choi EK, Kim KR, Shin JH and Lim JO: Temporary metallic stent placement in the treatment of refractory benign esophageal strictures: results and factors associated with outcome in 55 patients. Eur Radiol. 19:384–390. 2009. View Article : Google Scholar

14 

Albiero R, Adamian M, Kobayashi N, et al: Short- and intermediate-term results of (32) P radioactive beta-emitting stent implantation in patients with coronary artery disease: The Milan Dose-Response Study. Circulation. 101:18–26. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Fareh J, Martel R, Kermani P and Leclerc G: Cellular effects of beta-particle delivery on vascular smooth muscle cells and endothelial cells: a dose-response study. Circulation. 99:1477–1484. 1999. View Article : Google Scholar : PubMed/NCBI

16 

John M, Shroff S, Farb A and Virmani R: Local arterial responses to 32P beta-emitting stents. Cardiovasc Radiat Med. 2:143–150. 2001. View Article : Google Scholar

17 

Teirstein PS: Prevention of vascular restenosis with radiation. Tex Heart Inst J. 25:30–33. 1998.PubMed/NCBI

18 

Williams DO: Radiation vascular therapy: a novel approach to preventing restenosis. Am J Cardiol. 81:18E–20E. 1998. View Article : Google Scholar : PubMed/NCBI

19 

Ishiwata S, Robinson K, Chronos N, Crocker IR and King SB III: Irradiation and postangioplasty restenosis: a recent overview. Jpn Heart J. 41:541–570. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Marzocchi A, Marrozzini C, Piovaccari G, et al: Restenosis after coronary angioplasty: its pathogenesis and prevention. Cardiologia. 36(12 Suppl 1): 309–320. 1991.(In Italian). PubMed/NCBI

21 

Chen H, Bao Y, Yu L, Jia R, Cheng W and Shao C: Comparison of cellular damage response to low-dose-rate 125I seed irradiation and high-dose-rate gamma irradiation in human lung cancer cells. Brachytherapy. 11:149–156. 2012. View Article : Google Scholar

22 

Zhuang HQ, Wang JJ, Liao AY, Wang JD and Zhao Y: The biological effect of 125I seed continuous low dose rate irradiation in CL187 cells. J Exp Clin Cancer Res. 28:122009. View Article : Google Scholar : PubMed/NCBI

23 

Liao A, Wang J, Wang J, Zhuang H and Zhao Y: Relative biological effectiveness and cell-killing efficacy of continuous low-dose-rate 125I seeds on prostate carcinoma cells in vitro. Integr Cancer Ther. 9:59–65. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Zamorano L, Yakar D, Dujovny M, Sheehan M and Kim J: Permanent iodine-125 implant and external beam radiation therapy for the treatment of malignant brain tumors. Stereotact Funct Neurosurg. 59:183–192. 1992. View Article : Google Scholar : PubMed/NCBI

25 

Ma ZH, Yang Y, Zou L and Luo KY: 125I seed irradiation induces up-regulation of the genes associated with apoptosis and cell cycle arrest and inhibits growth of gastric cancer xenografts. J Exp Clin Cancer Res. 31:612012. View Article : Google Scholar : PubMed/NCBI

26 

Goodpaster T, Legesse-Miller A, Hameed MR, Aisner SC, Randolph-Habecker J and Coller HA: An immunohistochemical method for identifying fibroblasts in formalin-fixed, paraffin-embedded tissue. J Histochem Cytochem. 56:347–358. 2008. View Article : Google Scholar

27 

Aird EG, Folkard M, Mayes CR, Bownes PJ, Lawson JM and Joiner MC: A purpose-built iodine-125 irradiation plaque for low dose rate low energy irradiation of cell lines in vitro. Br J Radiol. 74:56–61. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Macheboeuf M, Basset J, Barbu E, Le Saget M and Nunez G: Effect of pressure on the hydrolysis of proteins by acids or alkalis. C R Seances Soc Biol Fil. 144:962–964. 1950.(In French). PubMed/NCBI

29 

Meredith WJ: Radium dosage-The Marchester system. E&S Livingstone Ltd; Edinburgh: 1947

30 

He QM and Wu XT: Experimental study on apoptosis of gastric cancer cell line MKN45 induced by continuous irradiation from iodine-125seeds. J Sichuan Univ Med Sci Ed. 40:454–458. 2009.(In Chinese).

31 

Wang J, Zhang H, Zhuang H, Zhao Y and Liao A: Development and validation of radioactive iodine-125 irradiator in vitro. Chin J Radiol Med Protect. 27:267–271. 2007.

32 

Vávrová J, Rezácová M, Vokurková D and Psutka J: Cell cycle alteration, apoptosis and response of leukemic cell lines to gamma radiation with high- and low-dose rate. Physiol Res. 53:335–342. 2004.PubMed/NCBI

33 

Mirzaie-Joniani H, Eriksson D, Johansson A, et al: Apoptosis in HeLa Hep2 cells is induced by low-dose, low-dose-rate radiation. Radiat Res. 158:634–640. 2002. View Article : Google Scholar : PubMed/NCBI

34 

Zhang YE, Xu ZD and Wang XH: Dynamic changes in extracellular matrix and related interstitial calls in experimental organ sclerosis. Chin J Pathol. 23:111–114. 1994.(In Chinese).

35 

Kovacs EJ and DiPietro LA: Fibrogenic cytokines and connective tissue production. FASEB J. 8:854–861. 1994.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Gan Z, Jing J, Zhu G, Qin Y, Teng G and Guo J: Preventive effects of 125I seeds on benign restenosis following esophageal stent implantation in a dog model. Mol Med Rep 11: 3382-3390, 2015.
APA
Gan, Z., Jing, J., Zhu, G., Qin, Y., Teng, G., & Guo, J. (2015). Preventive effects of 125I seeds on benign restenosis following esophageal stent implantation in a dog model. Molecular Medicine Reports, 11, 3382-3390. https://doi.org/10.3892/mmr.2014.3130
MLA
Gan, Z., Jing, J., Zhu, G., Qin, Y., Teng, G., Guo, J."Preventive effects of 125I seeds on benign restenosis following esophageal stent implantation in a dog model". Molecular Medicine Reports 11.5 (2015): 3382-3390.
Chicago
Gan, Z., Jing, J., Zhu, G., Qin, Y., Teng, G., Guo, J."Preventive effects of 125I seeds on benign restenosis following esophageal stent implantation in a dog model". Molecular Medicine Reports 11, no. 5 (2015): 3382-3390. https://doi.org/10.3892/mmr.2014.3130
Copy and paste a formatted citation
x
Spandidos Publications style
Gan Z, Jing J, Zhu G, Qin Y, Teng G and Guo J: Preventive effects of 125I seeds on benign restenosis following esophageal stent implantation in a dog model. Mol Med Rep 11: 3382-3390, 2015.
APA
Gan, Z., Jing, J., Zhu, G., Qin, Y., Teng, G., & Guo, J. (2015). Preventive effects of 125I seeds on benign restenosis following esophageal stent implantation in a dog model. Molecular Medicine Reports, 11, 3382-3390. https://doi.org/10.3892/mmr.2014.3130
MLA
Gan, Z., Jing, J., Zhu, G., Qin, Y., Teng, G., Guo, J."Preventive effects of 125I seeds on benign restenosis following esophageal stent implantation in a dog model". Molecular Medicine Reports 11.5 (2015): 3382-3390.
Chicago
Gan, Z., Jing, J., Zhu, G., Qin, Y., Teng, G., Guo, J."Preventive effects of 125I seeds on benign restenosis following esophageal stent implantation in a dog model". Molecular Medicine Reports 11, no. 5 (2015): 3382-3390. https://doi.org/10.3892/mmr.2014.3130
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