MicroRNA-33a inhibits lung cancer cell proliferation and invasion by regulating the expression of β-catenin

Zhu,Changliang; Zhao,Yachao; Zhang,Zhipei; Ni,Yunfeng; Li,Xiaofei; Yong,Han

doi:10.3892/mmr.2014.3134

MicroRNA-33a inhibits lung cancer cell proliferation and invasion by regulating the expression of β-catenin

Authors:
- Changliang Zhu
- Yachao Zhao
- Zhipei Zhang
- Yunfeng Ni
- Xiaofei Li
- Han Yong
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Affiliations: Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China, Department of Thoracic Surgery, The 309th Hospital of the Chinese PLA, Beijing 100091, P.R. China
Published online on: December 24, 2014 https://doi.org/10.3892/mmr.2014.3134
Pages: 3647-3651

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Abstract

MicroRNAs (miRNAs) are short, non‑coding RNAs that are aberrantly expressed in tumors. miRNA‑33a (miR‑33a) is closely associated with cholesterol metabolism and is essential for cellular growth. The aim of the present study was to explore the role of miR‑33a and identify its clinical significance in lung cancer cells. miR‑33a was observed to be overexpressed in the lung cancer cell lines A549 and NCI‑H460. MTT assay results demonstrated that the overexpression of miR‑33a significantly inhibited the proliferation of A549 cells, and similar results were obtained from the colony formation assay. This suggests that transfection of miR‑33a may suppress the growth of lung cancer cells. Overexpression of miR‑33a was also observed to result in marked G1/S phase cell cycle arrest in A549 and NCI‑H460 cell lines using fluorescence‑activated cell sorting analysis. Western blot analysis revealed that overexpression of miR‑33a significantly reduced the expression of β‑catenin in A549 and NCI‑H460 cells, suggesting a direct or indirect regulation of β‑catenin by miR‑33a in lung cancer cells. In conclusion, the current study may provide strategies for the treatment of lung cancer and clarify the mechanism of its progression.

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