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Article

Inhibitory effect and mechanisms of microRNA-146b-5p on the proliferation and metastatic potential of Caski human cervical cancer cells

  • Authors:
    • Cuiping Shen
    • Hui Yang
    • Hong Liu
    • Xiuqin Wang
    • Youzhong Zhang
    • Rui Xu
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Gynecology and Obstetrics, Rizhao People's Hospital, Rizhao, Shandong 276826, P.R. China
  • Pages: 3955-3961
    |
    Published online on: January 7, 2015
       https://doi.org/10.3892/mmr.2015.3151
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Abstract

Cervical cancer is a common cause of cancer‑associate mortality in females, and metastasis is strongly associated with failure of cervical cancer treatment. Previous studies have indicated that microRNA (miR)‑146b‑5p is involved in the inhibition of proliferation and metastasis of numerous human cancer types. The aim of the present study was to explore the inhibitory effect of miR‑156b‑5p on the proliferation and metastatic potential of Caski human cervical cancer cells, as well as to determine the mechanisms by which it proceeds. The results demonstrated that miR‑146b‑5p was able to inhibit the proliferative, invasive and adhesive potential and block the cell cycle progression of Caski human cervical cancer cells, as determined using MTS and transwell assays as well as flow cytometry. Furthermore, quantitative polymerase chain reaction and western blot analysis revealed that transfection with miR‑146b‑5p decreased the mRNA and protein expression levels of C‑X‑C chemokine receptor type 4, matrix metalloproteinase‑2 and ‑9, c‑Myc, cyclin D1 and human papilloma virus 16. In addition, the secretion levels of transforming growth factor‑β, monocyte chemoattractant protein‑1 and tumor necrosis factor‑α, the telomerase activity, the phosphorylation of c‑Jun N‑terminal protein kinase and protein kinase B and the transcriptional activities of nuclear factor‑κB, signal transducer and activator of transcription‑3 and ‑5 were reduced. However, increased levels of p27 and p53 were detected in the miR‑146b‑5p‑overexpressing Caski cells. These results indicate that miR‑146b‑5p may be a potential therapeutic strategy for the treatment of cervical cancer through regulation of cell chemotaxis and the cell cycle.
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1 

Sriplung H, Singkham P, Iamsirithaworn S, Jiraphongsa C and Bilheem S: Success of a cervical cancer screening program: trends in incidence in songkhla, southern Thailand, 1989–2010, and prediction of future incidences to 2030. Asian Pac J Cancer Prev. 15:10003–10008. 2014. View Article : Google Scholar

2 

Üreyen I, Aksoy Ü, Dündar B, Tapisiz ÖL, Karalök MA, Turan AT, Boran N and Tulunay HG: Does lymph node involvement affect the patterns of recurrence in stage IB cervical cancer? Turk J Med Sci. 44:844–852. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Chawla N, Breen N, Liu B, Lee R and Kagawa-Singer M: Asian American Women in California: a pooled analysis of predictors for breast and cervical cancer screening. Am J Public Health. 18:e1–e12. 2014.

4 

Li C, Ma C, Zhang W and Wang J: The immune function differences and high-risk human papillomavirus infection in the progress of cervical cancer. Eur J Gynaecol Oncol. 5:557–561. 2014.

5 

Bai LX, Wang JT, Ding L, Jiang SW, Kang HJ, Gao CF, Chen X, Chen C and Zhou Q: Folate deficiency and FHIT hypermethylation and HPV 16 infection promote cervical cancerization. Asian Pac J Cancer Prev. 21:9313–9317. 2014. View Article : Google Scholar

6 

García-Zepeda SP, García-Villa E, Díaz-Chávez J, Hernández-Pando R and Gariglio P: Resveratrol induces cell death in cervical cancer cells through apoptosis and autophagy. Eur J Cancer Prev. 22:577–584. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Gocze K, Gombos K, Kovacs K, Juhasz K, Gocze P and Kiss I: MicroRNA expressions in HPV-induced cervical dysplasia and cancer. Anticancer Res. 1:523–530. 2015.

8 

Shishodia G, Verma G, Srivastava Y, Mehrotra R, Das BC and Bharti AC: Deregulation of microRNAs Let-7a and miR-21 mediate aberrant STAT3 signaling during human papillomavirus-induced cervical carcinogenesis: role of E6 oncoprotein. BMC Cancer. 14:9962014.PubMed/NCBI

9 

Ribeiro J and Sousa H: MicroRNAs as biomarkers of cervical cancer development: a literature review on miR-125b and miR-34a. Mol Biol Rep. 3:1525–1531. 2014. View Article : Google Scholar

10 

Wang X, Wang HK, Li Y, Hafner M, Banerjee NS, Tang S, Briskin D, Meyers C, Chow LT, et al: MicroRNAs are biomarkers of oncogenic human papillomavirus infections. Proc Natl Acad Sci USA. 11:4262–4267. 2014. View Article : Google Scholar

11 

Kutty RK, Nagineni CN, Samuel W, Vijayasarathy C, Jaworski C, Duncan T, Cameron JE, Flemington EK, Hooks JJ and Redmond TM: Differential regulation of microRNA-146a and microRNA-146b-5p in human retinal pigment epithelial cells by interleukin-1β, tumor necrosis factor-α, and interferon-γ. Mol Vis. 19:737–750. 2013.

12 

Li Y, Wang Y, Yu L, Sun C, Cheng D, Yu S, Wang Q, Yan Y, Kang C, Jin S, An T, Shi C, Xu J, Wei C, Liu J, Sun J, Wen Y, Zhao S and Kong Y: miR-146b-5p inhibits glioma migration and invasion by targeting MMP16. Cancer Lett. 339:260–269. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Bergot AS, Ford N, Leggatt GR, Wells JW, Frazer IH and Grimbaldeston MA: HPV16-E7 expression in squamous epithelium creates a local immune suppressive environment via CCL2- and CCL5- mediated recruitment of mast cells. PLoS Pathog. 10:e10044662014. View Article : Google Scholar : PubMed/NCBI

14 

Yu Y, Zhang Y and Zhang S: MicroRNA-92 regulates cervical tumorigenesis and its expression is upregulated by human papilloma virus-16 E6 in cervical cancer cells. Oncol Lett. 6:468–474. 2013.PubMed/NCBI

15 

Zhu Y, Zhang L, Zhang GD, Wang HO, Liu MY, Jiang Y, Qi LS, Li Q and Yang P: Potential mechanisms of benzyl isothiocyanate suppression of invasion and angiogenesis by the U87MG human glioma cell line. Asian Pac J Cancer Prev. 19:8225–8228. 2014. View Article : Google Scholar

16 

Li J, Jiang K, Qiu X, Li M, Hao Q, Wei L, Zhang W, Chen B and Xin X: Overexpression of CXCR4 is significantly associated with cisplatin-based chemotherapy resistance and can be a prognostic factor in epithelial ovarian cancer. BMB Rep. 47:33–38. 2014. View Article : Google Scholar :

17 

Nowak E, Galilejczyk A, Sypniewski D and Bednarek I: MMP-9 directed shRNAs as relevant inhibitors of matrix metalloproteinase 9 activity and signaling. Postepy Hig Med Dosw (Online). 67:742–749. 2013. View Article : Google Scholar

18 

Gutschalk CM, Yanamandra AK, Linde N, Meides A, Depner S and Mueller MM: GM-CSF enhances tumor invasion by elevated MMP-2, -9, and -26 expression. Cancer Med. 2:117–129. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Fan X, Wu W, Shi H and Han J: RNA interference targeting CD147 inhibits the invasion of human cervical squamous carcinoma cells by downregulating MMP-9. Cell Biol Int. 37:737–741. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Zhu Y, Zhu L, Lu L, Zhang L, Zhang G, Wang Q and Yang P: Role and mechanism of the alkylglycerone phosphate synthase in suppressing the invasion potential of human glioma and hepatic carcinoma cells in vitro. Oncol Rep. 1:431–436. 2014.

21 

Kuroda H, Jomen W, Miura S, Arihara Y, Yamada M, Hirako T, Abe T, Sakurai T, Fujii S, Maeda M, et al: Bendamustine-rituximab therapy is effective for transformed follicular lymphoma with significant expression of p53. Gan To Kagaku Ryoho. 40:1055–1058. 2013.(In Japanese). PubMed/NCBI

22 

Zhang M, Li J, Wang L, Tian Z, Zhang P, Xu Q, Zhang C, Wei F and Chen W: Prognostic significance of p21, p27 and survivin protein expression in patients with oral squamous cell carcinoma. Oncol Lett. 6:381–386. 2013.PubMed/NCBI

23 

Portari EA, Russomano FB, de Camargo MJ, Machado Gayer CR, da Rocha Guillobel HC, Santos-Rebouças CB and Brito Macedo JM: Immunohistochemical expression of cyclin D1, p16Ink4a, p21WAF1, and Ki-67 correlates with the severity of cervical neoplasia. Int J Gynecol Pathol. 32:501–508. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Sui X, Kong N, Wang Z and Pan H: Epigenetic regulation of the human telomerase reverse transciptase gene: A potential therapeutic target for the treatment of leukemia (Review). Oncol Lett. 6:317–322. 2013.PubMed/NCBI

25 

Kim MS, Bak Y, Park YS, Lee DH, Kim JH, Kang JW, Song HH, Oh SR and Yoon do Y: Wogonin induces apoptosis by suppressing E6 and E7 expressions and activating intrinsic signaling pathways in HPV-16 cervical cancer cells. Cell Biol Toxicol. 29:259–272. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Almajhdi FN, Senger T, Amer HM, Gissmann L and Ohlschläger P: Design of a highly effective therapeutic HPV16 E6/E7-Specific DNA vaccine: Optimization by different ways of sequence rearrangements (shuffling). PLoS One. 9:e1134612014. View Article : Google Scholar : PubMed/NCBI

27 

Masuda S, Kumano K, Shimizu K, Imai Y, Kurokawa M, Ogawa S, Miyagishi M, Taira K, Hirai H and Chiba S: Notch1 oncoprotein antagonizes TGF-beta/Smad-mediated cell growth suppression via sequestration of coactivator p300. Cancer Sci. 96:274–282. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Bhaumik D, Scott GK, Schokrpur S, Patil CK, Campisi J and Benz CC: Expression of microRNA-146 suppresses NF-kappaB activity with reduction of metastatic potential in breast cancer cells. Oncogene. 27:5643–5647. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Geraldo MV, Yamashita AS and Kimura ET: MicroRNA miR-146b-5p regulates signal transduction of TGF-β by repressing SMAD4 in thyroid cancer. Oncogene. 31:1910–1922. 2012. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Shen C, Yang H, Liu H, Wang X, Zhang Y and Xu R: Inhibitory effect and mechanisms of microRNA-146b-5p on the proliferation and metastatic potential of Caski human cervical cancer cells. Mol Med Rep 11: 3955-3961, 2015.
APA
Shen, C., Yang, H., Liu, H., Wang, X., Zhang, Y., & Xu, R. (2015). Inhibitory effect and mechanisms of microRNA-146b-5p on the proliferation and metastatic potential of Caski human cervical cancer cells. Molecular Medicine Reports, 11, 3955-3961. https://doi.org/10.3892/mmr.2015.3151
MLA
Shen, C., Yang, H., Liu, H., Wang, X., Zhang, Y., Xu, R."Inhibitory effect and mechanisms of microRNA-146b-5p on the proliferation and metastatic potential of Caski human cervical cancer cells". Molecular Medicine Reports 11.5 (2015): 3955-3961.
Chicago
Shen, C., Yang, H., Liu, H., Wang, X., Zhang, Y., Xu, R."Inhibitory effect and mechanisms of microRNA-146b-5p on the proliferation and metastatic potential of Caski human cervical cancer cells". Molecular Medicine Reports 11, no. 5 (2015): 3955-3961. https://doi.org/10.3892/mmr.2015.3151
Copy and paste a formatted citation
x
Spandidos Publications style
Shen C, Yang H, Liu H, Wang X, Zhang Y and Xu R: Inhibitory effect and mechanisms of microRNA-146b-5p on the proliferation and metastatic potential of Caski human cervical cancer cells. Mol Med Rep 11: 3955-3961, 2015.
APA
Shen, C., Yang, H., Liu, H., Wang, X., Zhang, Y., & Xu, R. (2015). Inhibitory effect and mechanisms of microRNA-146b-5p on the proliferation and metastatic potential of Caski human cervical cancer cells. Molecular Medicine Reports, 11, 3955-3961. https://doi.org/10.3892/mmr.2015.3151
MLA
Shen, C., Yang, H., Liu, H., Wang, X., Zhang, Y., Xu, R."Inhibitory effect and mechanisms of microRNA-146b-5p on the proliferation and metastatic potential of Caski human cervical cancer cells". Molecular Medicine Reports 11.5 (2015): 3955-3961.
Chicago
Shen, C., Yang, H., Liu, H., Wang, X., Zhang, Y., Xu, R."Inhibitory effect and mechanisms of microRNA-146b-5p on the proliferation and metastatic potential of Caski human cervical cancer cells". Molecular Medicine Reports 11, no. 5 (2015): 3955-3961. https://doi.org/10.3892/mmr.2015.3151
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