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Article

Pannexin‑1 silencing inhibits the proliferation of U87‑MG cells

  • Authors:
    • Li Wei
    • Xiaobo Yang
    • Xiaohong Shi
    • Yinghui Chen
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Jinshan Hospital, Fudan University, Shanghai 201508, P.R. China, Department of Endocrinology, Jinshan Hospital, Fudan University, Shanghai 201508, P.R. China
  • Pages: 3487-3492
    |
    Published online on: January 8, 2015
       https://doi.org/10.3892/mmr.2015.3169
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Abstract

Pannexin‑1 (Panx‑1) is abundantly expressed in vertebrates and has been shown to assemble into high‑conductance single‑membrane channels, which are permeable to large molecules and regulate cellular function. However, the association between Panx‑1 and astrocyte proliferation is poorly understood. This study provides evidence for a difference in cell proliferation between wild‑type and Panx‑1‑knockdown cells. Proliferation of the U87‑MG malignant glioma cell line was reduced following transfection with Panx‑1‑short interfering RNA. In addition, treatment with the Panx‑1 activator, adenosine triphosphate, significantly reduced cell proliferation at 48 h in Panx‑1‑knockdown cells compared with wild type cells. In conclusion, on the basis of the present findings, Panx‑1 is likely to be important in the regulation of U87‑MG cell proliferation. This provides further support for the hypothesis that there is a correlation between Panx‑1 expression and U87‑MG cell proliferation.
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1 

Barbe MT, Monyer H and Bruzzone R: Cell-cell communication beyond connexins: the pannexin channels. Physiology (Bethesda). 21:103–114. 2006. View Article : Google Scholar

2 

Panchin Y, Kelmanson I, Matz M, et al: A ubiquitous family of putative gap junction molecules. Curr Biol. 10:R473–R474. 2000. View Article : Google Scholar : PubMed/NCBI

3 

Suadicani SO, Iglesias R, Wang J, et al: ATP signaling is deficient in cultured Pannexin1-null mouse astrocytes. Glia. 60:1106–1116. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Dahl G and Keane RW: Pannexin: from discovery to bedside in 11±4 years? Brain Res. 3:150–159. 2012. View Article : Google Scholar

5 

Penuela S, Gehi R and Laird DW: The biochemistry and function of pannexin channels. Biochim Biophys Acta. 1828:15–22. 2013. View Article : Google Scholar

6 

D’hondt C, Ponsaerts R, De Smedt H, et al: Pannexin channels in ATP release and beyond: An unexpected rendezvous at the endoplasmic reticulum. Cell Signal. 23:305–316. 2011. View Article : Google Scholar

7 

Gulbransen BD, Bashashati M, Hirota SA, et al: Activation of neuronal P2×7 receptor-pannexin-1 mediates death of enteric neurons during colitis. Nat Med. 18:600–604. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Celetti SJ, Cowan KN, Penuela S, et al: Implications of pannexin 1 and pannexin 3 for keratinocyte differentiation. J Cell Sci. 123:1363–1372. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Orellana JA, Froger N, Ezan P, et al: ATP and glutamate released via astroglial connexin 43 hemichannels mediate neuronal death through activation of pannexin 1 hemichannels. J Neurochem. 118:826–840. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Pinheiro AR, Paramos-de-Carvalho D, Certal M, et al: Histamine induces ATP release from human subcutaneous fibroblasts, via pannexin-1 hemichannels, leading to Ca2+ mobilization and cell proliferation. J Biol Chem. 288:27571–27583. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Wicki-Stordeur LE, Dzugalo AD, Swansburg RM, et al: Pannexin 1 regulates postnatal neural stem and progenitor cell proliferation. Neural Devel. 7:112012. View Article : Google Scholar

12 

Lai CP, Bechberger JF, Thompson RJ, et al: Tumor-suppressive effects of pannexin 1 in C6 glioma cells. Cancer Res. 67:1545–1554. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Iwamoto T, Nakamura T, Doyle A, et al: Pannexin 3 regulates intracellular ATP/cAMP levels and promotes chondrocyte differentiation. J Biol Chem. 285:18948–18958. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Bond SR, Lau A, Penuela S, et al: Pannexin 3 is a novel target for Runx2, expressed by osteoblasts and mature growth plate chondrocytes. J Bone Miner Res. 26:2911–2922. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Turmel P, Dufresne J, Hermo L, et al: Characterization of pannexin1 and pannexin3 and their regulation by androgens in the male reproductive tract of the adult rat. Mol Reprod Dev. 78:124–138. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Chekeni FB, Elliott MR, Sandilos JK, et al: Pannexin 1 channels mediate ‘find-me’ signal release and membrane permeability during apoptosis. Nature. 467:863–867. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Tan C, Voss U, Svensson S, et al: High glucose and free fatty acids induce beta cell apoptosis via autocrine effects of ADP acting on the P2Y(13) receptor. Purinergic Signal. 9:67–79. 2013. View Article : Google Scholar :

18 

Santiago MF, Veliskova J, Patel NK, et al: Targeting pannexin1 improves seizure outcome. PLoS One. 6:e251782011. View Article : Google Scholar : PubMed/NCBI

19 

Karpuk N, Burkovetskaya M, Fritz T, et al: Neuroinflammation leads to region-dependent alterations in astrocyte gap junction communication and hemichannel activity. J Neurosci. 31:14–425. 2011. View Article : Google Scholar

20 

Stoimenov I and Helleday T: PCNA on the crossroad of cancer. Biochem Soc Trans. 37:605–613. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Chiara AD, Pederzoli-Ribeil M, Burgel PR, et al: Targeting cytosolic proliferating cell nuclear antigen in neutrophil-dominated inflammation. Front Immunol. 3:3112012. View Article : Google Scholar : PubMed/NCBI

22 

Strzalka W and Ziemienowicz A: Proliferating cell nuclear antigen (PCNA): a key factor in DNA replication and cell cycle regulation. Ann Bot. 107:1127–1140. 2011. View Article : Google Scholar :

23 

de Sousa Abreu R, Penalva LO, Marcotte EM, et al: Global signatures of protein and mRNA expression levels. Mol Biosyst. 5:1512–1526. 2009.PubMed/NCBI

24 

Ryten M, Dunn PM, Neary JT and Burnstock G: ATP regulates the differentiation of mammalian skeletal muscle by activation of a P2×5 receptor on satellite cells. J Cell Biol. 158:345–355. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Ryten M, Yang SY, Dunn PM, et al: Purinoceptor expression in regenerating skeletal muscle in the mdx mouse model of muscular dystrophy and in satellite cell cultures. FASEB J. 18:1404–1406. 2004.PubMed/NCBI

26 

Bao BA, Lai CP, Naus CC and Morgan JR: Pannexin1 drives multicellular aggregate compaction via a signaling cascade that remodels the actin cytoskeleton. J Biol Chem. 287:8407–8416. 2012. View Article : Google Scholar : PubMed/NCBI

27 

McKuen MJ, Dahl G and Fields KA: Assessing a potential role of host Pannexin 1 during Chlamydia trachomatis infection. PLoS One. 8:e637322013. View Article : Google Scholar : PubMed/NCBI

28 

Wang L, Zhu R, Huang Z, et al: Lipopolysaccharide-induced toll-like receptor 4 signaling in cancer cells promotes cell survival and proliferation in hepatocellular carcinoma. Dig Dis Sci. 58:2223–2236. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Chen YH, Wang CC, Xiao X, et al: Multidrug resistance-associated protein 1 decreases the concentrations of antiepileptic drugs in cortical extracellular fluid in amygdale kindling rats. Acta Pharmacol Sin. 34:473–479. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Tietje K, Rivera-Ingraham G, Petters C, et al: Reporter dyes demonstrate functional expression of multidrug resistance proteins in the marine flatworm Macrostomum lignano: the sponge-derived dye Ageladine A is not a substrate of these transporters. Mar Drugs. 11:3951–3969. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Furugen A, Yamaguchi H, Tanaka N, et al: Contribution of multidrug resistance-associated proteins (MRPs) to the release of prostanoids from A549 cells. Prostaglandins Other Lipid Mediat. 106:37–44. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Wu J, Huang W and He Z: Dendrimers as carriers for siRNA delivery and gene silencing: a review. ScientificWorldJournal. 29:6306542013.

33 

Wilson RC and Doudna JA: Molecular mechanisms of RNA interference. Annu Rev Biophys. 42:217–239. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Xu XW, Ding BW, Zhu CR, et al: PU.1-silenced dendritic cells prolong allograft survival in rats receiving intestinal transplantation. World J Gastroenterol. 19(43): 7766–7771. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Kang ZH, Wang CY, Zhang WL, et al: Histone deacetylase HDAC4 promotes gastric cancer SGC-7901 cells progression via p21 repression. PLoS One. 9:e988942014. View Article : Google Scholar : PubMed/NCBI

36 

Zhou M, Zhou L, Zheng L, et al: miR-365 Promotes Cutaneous Squamous Cell Carcinoma (CSCC) through Targeting Nuclear Factor I/B (NFIB). PLoS One. 9:e1006202014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wei L, Yang X, Shi X and Chen Y: Pannexin‑1 silencing inhibits the proliferation of U87‑MG cells. Mol Med Rep 11: 3487-3492, 2015.
APA
Wei, L., Yang, X., Shi, X., & Chen, Y. (2015). Pannexin‑1 silencing inhibits the proliferation of U87‑MG cells. Molecular Medicine Reports, 11, 3487-3492. https://doi.org/10.3892/mmr.2015.3169
MLA
Wei, L., Yang, X., Shi, X., Chen, Y."Pannexin‑1 silencing inhibits the proliferation of U87‑MG cells". Molecular Medicine Reports 11.5 (2015): 3487-3492.
Chicago
Wei, L., Yang, X., Shi, X., Chen, Y."Pannexin‑1 silencing inhibits the proliferation of U87‑MG cells". Molecular Medicine Reports 11, no. 5 (2015): 3487-3492. https://doi.org/10.3892/mmr.2015.3169
Copy and paste a formatted citation
x
Spandidos Publications style
Wei L, Yang X, Shi X and Chen Y: Pannexin‑1 silencing inhibits the proliferation of U87‑MG cells. Mol Med Rep 11: 3487-3492, 2015.
APA
Wei, L., Yang, X., Shi, X., & Chen, Y. (2015). Pannexin‑1 silencing inhibits the proliferation of U87‑MG cells. Molecular Medicine Reports, 11, 3487-3492. https://doi.org/10.3892/mmr.2015.3169
MLA
Wei, L., Yang, X., Shi, X., Chen, Y."Pannexin‑1 silencing inhibits the proliferation of U87‑MG cells". Molecular Medicine Reports 11.5 (2015): 3487-3492.
Chicago
Wei, L., Yang, X., Shi, X., Chen, Y."Pannexin‑1 silencing inhibits the proliferation of U87‑MG cells". Molecular Medicine Reports 11, no. 5 (2015): 3487-3492. https://doi.org/10.3892/mmr.2015.3169
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