search button

Effects of the JWA gene in the regulation of human breast cancer cells

  • Authors:
    • Xiang Chen
    • Jiake Feng
    • Zhijun Ge
    • Hong Chen
    • Weiliang Ding
    • Wenjiao Zhu
    • Xiaoyan Tang
    • Yanyu Chen
    • Yongfei Tan
    • Tieliang Ma

  • View Affiliations

  • Published online on: January 13, 2015     https://doi.org/10.3892/mmr.2015.3188
  • Pages: 3848-3853
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study aimed to investigate whether the JWA gene can regulate the proliferation, migration and invasion of human breast cancer cells through the MAPK signaling pathway. The role of JWA in proliferation, migration, invasion and apoptosis was investigated in the MDA‑MB‑231 human breast cancer cell line. Following transfection with JWA‑small interfering (si)RNA, the effect of JWA on apoptosis was assessed by Western blot analysis, proliferation was determined using Transwell chambers and cell migration and invasion were analyzed by transwell assay. The expression levels of extracellular signal‑regulated kinase (ERK) 1/2, CSBP/RK/Mpk2 kinase (p38) and c‑Jun N‑terminal kinase (JNK) were detected using Western blot analysis in the siRNA and control groups. The expression of JWA in the breast cancer cells was significantly lower compared with the normal breast cells. Downregulation of JWA protein levels reduced the apoptosis and enhanced proliferation, migration and invasion of the MDA‑MB‑231 cells in vitro. The results of the Western blot analysis demonstrated that, compared with the control groups, the expression levels of phosphorylated (p‑)p38 decreased significantly in the JWA siRNA group. No significant changes were observed in the expression levels of p‑ERK1/2 or p‑JNK. Therefore, the JWA gene may regulate human breast cancer cells through the MAPK signaling pathway using different types of regulation.
View Figures
View References

Related Articles

Journal Cover

May-2015
Volume 11 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 6.9
  • CiteScore Rank: Q2: #98/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.721
  • SCImago Journal Rank (SJR): 0.65
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Feng J, Ge Z, Chen H, Ding W, Zhu W, Tang X, Chen Y, Tan Y, Ma T, Ma T, et al: Effects of the JWA gene in the regulation of human breast cancer cells. Mol Med Rep 11: 3848-3853, 2015
APA
Chen, X., Feng, J., Ge, Z., Chen, H., Ding, W., Zhu, W. ... Ma, T. (2015). Effects of the JWA gene in the regulation of human breast cancer cells. Molecular Medicine Reports, 11, 3848-3853. https://doi.org/10.3892/mmr.2015.3188
MLA
Chen, X., Feng, J., Ge, Z., Chen, H., Ding, W., Zhu, W., Tang, X., Chen, Y., Tan, Y., Ma, T."Effects of the JWA gene in the regulation of human breast cancer cells". Molecular Medicine Reports 11.5 (2015): 3848-3853.
Chicago
Chen, X., Feng, J., Ge, Z., Chen, H., Ding, W., Zhu, W., Tang, X., Chen, Y., Tan, Y., Ma, T."Effects of the JWA gene in the regulation of human breast cancer cells". Molecular Medicine Reports 11, no. 5 (2015): 3848-3853. https://doi.org/10.3892/mmr.2015.3188