Human umbilical cord and dental pulp-derived mesenchymal stem cells: Biological characteristics and potential roles in vitro and in vivo

Cui,Xiaoyan; Chen,Lei; Xue,Ting; Yu,Jing; Liu,Jie; Ji,Yazhong; Cheng,Liming

doi:10.3892/mmr.2015.3198

Human umbilical cord and dental pulp-derived mesenchymal stem cells: Biological characteristics and potential roles in vitro and in vivo

Authors:
- Xiaoyan Cui
- Lei Chen
- Ting Xue
- Jing Yu
- Jie Liu
- Yazhong Ji
- Liming Cheng
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Affiliations: Translational Center for Stem Cell Research, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China, Department of Spine Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China, Department of Reproductive Medicine, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China
Published online on: January 14, 2015 https://doi.org/10.3892/mmr.2015.3198
Pages: 3269-3278
Copyright: © Cui et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Mesenchymal stem/stromal cells (MSCs) have a wide application in cell‑based therapies and tissue engineering. In the present study, the differentiation, survivin (SVV)‑modified effects and molecular basis of human umbilical cord‑derived MSCs (HUMSCs) and dental pulp‑derived stem cells (DPSCs) were investigated. The HUMSCs were found to differentiate into adipocytes more readily than the DPSCs and the HUMSCs and DPSCs were each able to differentiate into osteoblasts and chondroblasts. Following modification of the MSCs by SVV, the secretion of SVV in the modified HUMSCs was significantly higher compared with that in the modified DPSCs. In vivo, survival of the SVV‑modified DPSCs was observed at 4 and 14 days after intrastriatal transplantation, as was the expression of SVV and differentiation into astrocytes. The gene expression profiles of the control and modified HUMSCs and DPSCs were compared using RNA sequencing and an association was observed between gene expression and variability in cell line function. These findings provide novel information regarding the differences between HUMSCs and DPSCs and insight into optimal cell sources for therapeutic applications.

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1	Choong PF, Mok PL, Cheong SK, Leong CF and Then KY: Generating neuron-like cells from BM-derived mesenchymal stromal cells in vitro. Cytotherapy. 9:170–183. 2007. View Article : Google Scholar : PubMed/NCBI
2	Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI
3	Xu R, Jiang X, Guo Z, Chen J, Zou Y, Ke Y, Zhang S, Li Z, Cai Y, Du M, Qin L, Tang Y and Zeng Y: Functional analysis of neuron-like cells differentiated from neural stem cells derived from bone marrow stroma cells in vitro. Cell Mol Neurobiol. 28:545–558. 2008. View Article : Google Scholar
4	Cui X, Chen L, Ren Y, Ji Y, Liu W, Liu J, Yan Q, Cheng L and Sun YE: Genetic modification of mesenchymal stem cells in spinal cord injury repair strategies. Biosci Trends. 7:202–208. 2013.PubMed/NCBI
5	Kode JA, Mukherjee S, Joglekar MV and Hardikar AA: Mesenchymal stem cells: immunobiology and role in immunomodulation and tissue regeneration. Cytotherapy. 11:377–391. 2009. View Article : Google Scholar : PubMed/NCBI
6	da Meirelles LS, Fontes AM, Covas DT and Caplan AI: Mechanisms involved in the therapeutic properties of mesenchymal stem cells. Cytokine Growth Factor Rev. 20:419–427. 2009. View Article : Google Scholar
7	Davies OG, Smith AJ, Cooper PR, Shelton RM and Scheven BA: The effects of cryopreservation on cells isolated from adipose, bone marrow and dental pulp tissues. Cryobiology. 69:342–347. 2014. View Article : Google Scholar : PubMed/NCBI
8	Bakhshi T, Zabriskie RC, Bodie S, Kidd S, Ramin S, Paganessi LA, Gregory SA, Fung HC and Christopherson KW II: Mesenchymal stem cells from the Wharton’s jelly of umbilical cord segments provide stromal support for the maintenance of cord blood hematopoietic stem cells during long-term ex vivo culture. Transfusion. 48:2638–2644. 2008. View Article : Google Scholar : PubMed/NCBI
9	Moretti P, Hatlapatka T, Marten D, Lavrentieva A, Majore I, Hass R and Kasper C: Mesenchymal stromal cells derived from human umbilical cord tissues: primitive cells with potential for clinical and tissue engineering applications. Adv Biochem Eng Biotechnol. 123:29–54. 2010.
10	Lisianyĭ MI: Mesenchymal stem cells and their immunological properties. Fiziol Zh. 59:126–134. 2013.(In Ukranian).
11	Yoo KH, Jang IK, Lee MW, Kim HE, Yang MS, Eom Y, Lee JE, Kim YJ, Yang SK, Jung HL, et al: Comparison of immunomodulatory properties of mesenchymal stem cells derived from adult human tissues. Cell Immunol. 259:150–156. 2009. View Article : Google Scholar : PubMed/NCBI
12	Yang CC, Shih YH, Ko MH, Hsu SY, Cheng H and Fu YS: Transplantation of human umbilical mesenchymal stem cells from Wharton’s jelly after complete transection of the rat spinal cord. PLoS One. 3:e33362008. View Article : Google Scholar
13	Ma H, Wu Y, Xu Y, Sun L and Zhang X: Human umbilical mesenchymal stem cells attenuate the progression of focal segmental glomerulosclerosis. Am J Med Sci. 346:486–493. 2013. View Article : Google Scholar : PubMed/NCBI
14	Gronthos S, Mankani M, Brahim J, Robey PG and Shi S: Postnatal human dental pulp stemcells (DPSCs) in vitro and in vivo. Proc Natl Acadm Sci USA. 97:13625–13630. 2000. View Article : Google Scholar
15	Zhao Y, Wang L, Jin Y and Shi S: Fas ligand regulates the immunomodulatory properties of dental pulp stem cells. J Dent Res. 91:948–954. 2012. View Article : Google Scholar : PubMed/NCBI
16	Kermani AJ, Fathi F and Mowla SJ: Characterization and genetic manipulation of human umbilical cord vein mesenchymal stem cells: potential application in cell-based gene therapy. Rejuvenation Res. 11:379–386. 2008. View Article : Google Scholar : PubMed/NCBI
17	Bhowmick R and Girotti AW: Cytoprotective signaling associated with nitric oxide upregulation in tumor cells subjected to photodynamic therapy-like oxidative stress. Free Radic Biol Med. 57:39–48. 2013. View Article : Google Scholar :
18	Kikuchi-Taura A, Taguchi A, Kanda T, Inoue T, Kasahara Y, Hirose H, Sato I, Matsuyama T, Nakagomi T, Yamahara K, et al: Human umbilical cord provides a significant source of unexpanded mesenchymal stromal cells. Cytotherapy. 14:441–450. 2012. View Article : Google Scholar : PubMed/NCBI
19	Fink KD, Rossignol J, Crane AT, Davis KK, Bombard MC, Bavar AM, Clerc S, Lowrance SA, Song C, Lescaudron L and Dunbar GL: Transplantation of umbilical cord-derived mesenchymal stem cells into the striata of R6/2 mice: behavioral and neuropathological analysis. Stem Cell Res Ther. 4:1302013. View Article : Google Scholar :
20	Trapnell C, Pachter L and Salzberg SL: TopHat: discovering splice junctions with RNA-Seq. Bioinformatics. 25:1105–1111. 2009. View Article : Google Scholar : PubMed/NCBI
21	Trapnell C, Roberts A, Goff L, Pertea G, Kim D, Kelley DR, Pimentel H, Salzberg SL, Rinn JL and Pachter L: Differential geneand transcript expressionanalysis of RNA-seq experiments with TopHat and Cufflinks. Nat Protoc. 7:562–578. 2012. View Article : Google Scholar : PubMed/NCBI
22	Baron F and Storb R: Mesenchymal stromal cells: a new tool against graft-versus-host disease? Biol Blood Marrow Transplant. 18:822–840. 2012. View Article : Google Scholar :
23	Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop DJ and Horwitz E: Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy. 8:315–317. 2006. View Article : Google Scholar : PubMed/NCBI
24	Mangano C, De Rosa A, Desiderio V, d’Aquino R, Piattelli A, De Francesco F, Tirino V, Mangano F and Papaccio G: The osteoblastic differentiation of dental pulp stem cells and bone formation on different titanium surface textures. Biomaterials. 31:3543–3551. 2010. View Article : Google Scholar : PubMed/NCBI
25	Fukuda S, Foster RG, Porter SB and Pelus LM: The antiapoptosis protein survivin is associated with cell cycle entry of normal cord blood CD34(+) cells and modulates cell cycle and proliferation of mouse hematopoietic progenitor cells. Blood. 100:2463–2471. 2002. View Article : Google Scholar : PubMed/NCBI