Role of osteopontin in the regulation of human bladder cancer proliferation and migration in T24 cells

  • Authors:
    • Song‑Tao Xu
    • Chun Guo
    • Xiang Ding
    • Wen‑Juan Fan
    • Fu‑Hua Zhang
    • Wan‑Ling Xu
    • Yong‑Chao Ma
  • View Affiliations

  • Published online on: January 15, 2015     https://doi.org/10.3892/mmr.2015.3202
  • Pages: 3701-3707
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Abstract

Osteopontin (OPN), a secreted acid glycoprotein with a variety of functions, promotes tumor proliferation, differentiation, invasion and metastasis. The aim of the current study was to investigate whether OPN may serve as a potential therapeutic target for human bladder cancer. RNA interference (RNAi) was performed to downregulate the expression of the OPN gene in T24 human bladder cancer cells. The mRNA and protein expression levels of OPN following RNAi were determined using reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. In addition, the cell cycle progression, apoptosis and proliferation were investigated using by flow cytometric analysis and MTT assay. The cell invasion ability was measured using a Matrigel transwell assay. The mRNA and protein expression levels of OPN were found to be significantly downregulated following RNAi. The proliferation and invasion of T24 cells were significantly inhibited in vitro. In conclusion, RNAi‑targeting OPN may inhibit the proliferation, invasion and tumorigenicity of human bladder cancer cells. Therefore, OPN may serve as a potential therapeutic target for human bladder cancer.
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May-2015
Volume 11 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Xu ST, Guo C, Ding X, Fan WJ, Zhang FH, Xu WL and Ma YC: Role of osteopontin in the regulation of human bladder cancer proliferation and migration in T24 cells. Mol Med Rep 11: 3701-3707, 2015
APA
Xu, S., Guo, C., Ding, X., Fan, W., Zhang, F., Xu, W., & Ma, Y. (2015). Role of osteopontin in the regulation of human bladder cancer proliferation and migration in T24 cells. Molecular Medicine Reports, 11, 3701-3707. https://doi.org/10.3892/mmr.2015.3202
MLA
Xu, S., Guo, C., Ding, X., Fan, W., Zhang, F., Xu, W., Ma, Y."Role of osteopontin in the regulation of human bladder cancer proliferation and migration in T24 cells". Molecular Medicine Reports 11.5 (2015): 3701-3707.
Chicago
Xu, S., Guo, C., Ding, X., Fan, W., Zhang, F., Xu, W., Ma, Y."Role of osteopontin in the regulation of human bladder cancer proliferation and migration in T24 cells". Molecular Medicine Reports 11, no. 5 (2015): 3701-3707. https://doi.org/10.3892/mmr.2015.3202