Recombinant human vascular endothelial growth factor receptor 1 effectively inhibits angiogenesis in vivo

Wang,Jinliang; Shi,Minglei; Xi,Yongyi; Gao,Lihua; Zhang,Guanyi; Shao,Yong; Chen,Huipeng; Hu,Xianwen

doi:10.3892/mmr.2015.3229

May-2015 Volume 11 Issue 5

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May-2015 Volume 11 Issue 5

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Article

Recombinant human vascular endothelial growth factor receptor 1 effectively inhibits angiogenesis in vivo

Authors:
- Jinliang Wang
- Minglei Shi
- Yongyi Xi
- Lihua Gao
- Guanyi Zhang
- Yong Shao
- Huipeng Chen
- Xianwen Hu
View Affiliations / Copyright

Affiliations: Department of General Oncology, Chinese PLA General Hospital, Beijing 100853, P.R. China, Department of Cell Engineering, Beijing Institute of Biotechnology, Beijing 100071, P.R. China
Pages: 3432-3438
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Published online on: January 20, 2015

https://doi.org/10.3892/mmr.2015.3229
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Abstract

Vascular endothelial growth factor (VEGF) plays an important role in both physiological and pathological angiogenesis. VEGF receptor‑1 (VEGFR‑1) acts as a decoy VEGF receptor that enables the regulation of VEGF on the vascular endothelium. In the present study, the recombinant human VEGFR1D1‑3/Fc (rhVEGFR‑1), which contains key domains for VEGF binding, was cloned and expressed in Chinese hamster ovary (CHO) cells. The rhVEGFR‑1 protein was purified using protein‑A affinity chromatography. The molecular weight of rhVEGFR‑1 was found to be ~162 and 81 kD in non‑reducing and reducing SDS‑PAGE, respectively. The majority of the final protein products were in the dimeric conformation. Western blot analysis revealed that rhVEGFR‑1 was only capable of binding to the full glycan form of rhVEGF‑165 and rhVEGF‑121. The dissociation constant for the binding of rhVEGFR‑1 to VEGF‑165, detected using Biacore, was 285 pM. In addition, rhVEGFR‑1 inhibited the proliferation and migration of human microvascular endothelial cells. In vivo experiments also demonstrated that rhVEGFR‑1 inhibited chicken chorioallantoic membrane neovascularization and angiogenesis in nude mice. In conclusion, an anti‑angiogenic recombinant soluble VEGFR was expressed (up to 5 mg/l) in CHO cells and was shown to be capable of inhibiting neovascularization in vivo and in vitro.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Recombinant human vascular endothelial growth factor receptor 1 effectively inhibits angiogenesis in vivo

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