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Article

Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats

  • Authors:
    • Hongwei Li
    • Qi Wu
    • Long Xu
    • Xue Li
    • Jianmin Duan
    • Jingyan Zhan
    • Jing Feng
    • Xin Sun
    • Huaiyong Chen
  • View Affiliations / Copyright

    Affiliations: Graduate School, Tianjin Medical University, Tianjin 300070, P.R. China, Department of Basic Medicine Laboratory, Tianjin Institute of Respiratory Diseases, Haihe Hospital, Tianjin 300350, P.R. China, Respiratory Department, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Key Research Laboratory for Infectious Disease Prevention for State Administration of Traditional Chinese Medicine, Respiratory Department, Tianjin Institute of Respiratory Diseases, Haihe Hospital, Tianjin 300350, P.R. China
  • Pages: 4639-4644
    |
    Published online on: January 21, 2015
       https://doi.org/10.3892/mmr.2015.3234
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Abstract

Chronic obstructive pulmonary disease (COPD) is a major public health problem, and cigarette smoke (CS) is the primary risk factor. The pathology is often observed in the lung, but COPD is also associated with intestinal barrier disruption, although the underlying mechanisms are poorly understood. To address this, a CS‑exposed rat model was evaluated in the present study by analyzing small intestinal gene expression using reverse transcription‑quantitative polymerase chain reaction. CS exposure caused upregulation of the nicotinamide adenine dinucleotide phosphate‑oxidase subunits nox2 and p22phox in the small intestine, while the antioxidative enzyme superoxide dismutase was downregulated. CS exposure also increased bax expression and decreased bcl‑2 expression. This was associated with an elevation of hypoxia‑inducible factor (HIF)‑1α. Claudin‑1 was decreased and claudin‑2 increased, indicating a loosening of small intestinal tight junctions (TJs). These data suggest that during the development of COPD, HIF‑1α expression is altered in the small intestine, which may be associated with the increased oxidative stress and apoptosis, eventually resulting in disruption of the intestinal TJs.
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Copy and paste a formatted citation
Spandidos Publications style
Li H, Wu Q, Xu L, Li X, Duan J, Zhan J, Feng J, Sun X and Chen H: Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats. Mol Med Rep 11: 4639-4644, 2015.
APA
Li, H., Wu, Q., Xu, L., Li, X., Duan, J., Zhan, J. ... Chen, H. (2015). Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats. Molecular Medicine Reports, 11, 4639-4644. https://doi.org/10.3892/mmr.2015.3234
MLA
Li, H., Wu, Q., Xu, L., Li, X., Duan, J., Zhan, J., Feng, J., Sun, X., Chen, H."Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats". Molecular Medicine Reports 11.6 (2015): 4639-4644.
Chicago
Li, H., Wu, Q., Xu, L., Li, X., Duan, J., Zhan, J., Feng, J., Sun, X., Chen, H."Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats". Molecular Medicine Reports 11, no. 6 (2015): 4639-4644. https://doi.org/10.3892/mmr.2015.3234
Copy and paste a formatted citation
x
Spandidos Publications style
Li H, Wu Q, Xu L, Li X, Duan J, Zhan J, Feng J, Sun X and Chen H: Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats. Mol Med Rep 11: 4639-4644, 2015.
APA
Li, H., Wu, Q., Xu, L., Li, X., Duan, J., Zhan, J. ... Chen, H. (2015). Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats. Molecular Medicine Reports, 11, 4639-4644. https://doi.org/10.3892/mmr.2015.3234
MLA
Li, H., Wu, Q., Xu, L., Li, X., Duan, J., Zhan, J., Feng, J., Sun, X., Chen, H."Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats". Molecular Medicine Reports 11.6 (2015): 4639-4644.
Chicago
Li, H., Wu, Q., Xu, L., Li, X., Duan, J., Zhan, J., Feng, J., Sun, X., Chen, H."Increased oxidative stress and disrupted small intestinal tight junctions in cigarette smoke-exposed rats". Molecular Medicine Reports 11, no. 6 (2015): 4639-4644. https://doi.org/10.3892/mmr.2015.3234
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